Preparation of a JAZF1 protein polyclonal antibody and its potential role in broiler ascites syndrome

Gu, Yueming; Zhou, Changming; Guo, Xiaoquan; Huang, Cheng; Liu, Pei; Hu, Guoliang; Liu, Sanfeng; Li, Guyue; Yu, Zhuang; Wu, Cong; Xu, Zheng

doi:10.1016/j.ijbiomac.2022.02.190

Cited by 4 publications

(3 citation statements)

References 30 publications

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“…For animal immunization experiments, according to the method, the effective titer of antibody is that the ratio of positive serum to negative serum is greater than 2.1 [22][23][24]. The results showed that the anti-Mb seroprevalence was 100% and the strongest antiserum titer of Rabbit B was greater than 1:256,000 (Figure 1F).…”

Section: Purification Of Rat Mb Recombinant Protein and Production Of...mentioning

confidence: 99%

A Novel Rabbit Anti-Myoglobin Monoclonal Antibody’s Potential Application in Rhabdomyolysis Associated Acute Kidney Injury

Wang

Qiao

Han

et al. 2023

IJMS

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Myoglobin (Mb) is the main constituent of vertebrate skeletal muscle and myocardium and plays an essential role in oxygen binding, storage, transport, and earliest disease diagnosis. This study focuses on preparing the novel recombinant rabbit anti-Mb monoclonal antibody and applying it to a diagnosis of Mb deposition in rhabdomyolysis-associated acute kidney injury (RM-AKI). The full-length coding sequence of rat Mb was cloned and expressed, and the high-quality and titer rabbit anti-Mb polyclonal antibodies were produced by the immunogen His-Mb fusion protein. A new hybridoma cell was obtained by hybridoma screening technology. With the help of DNA sequencing and a molecular clonal, anti-Mb monoclonal antibody heavy and light chains expression plasmid was constructed. Finally, the recombinant rabbit anti-Mb monoclonal antibody with extraordinarily high affinity (KD = 1.21 pM) was obtained. Meanwhile, it had broad species reactivity (mouse, rat, human, and horse) and good tissue specificity (skeletal muscle and myocardium). It also had a very good performance in western blotting, immunohistochemistry, and immunofluorescence assay to detect the Mb level in the kidney, myocardium, and skeletal muscle of RM-AKI. This study will be significantly helpful for Mb-associated disease diagnosis, and pathogenesis exploration, and further may act as a neutralizing antibody for disease treatment.

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Section: Purification Of Rat Mb Recombinant Protein and Production Of...mentioning

confidence: 99%

A Novel Rabbit Anti-Myoglobin Monoclonal Antibody’s Potential Application in Rhabdomyolysis Associated Acute Kidney Injury

Wang

Qiao

Han

et al. 2023

IJMS

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“…MiR-181 induces pyroptosis by affecting the SIRT1/PGC-1a/Nrf2 signaling cascade reaction, which increases the levels of pyroptosis-associated proteins, such as NLRP3, cysteinyl asparagin-1, IL-1β and IL-18 (Zhao et al, 2019). Gu, Y. et al found that both overexpression of miR-200b and reduction of JAZF1 can lead to activation of the NF-κB signaling pathway, which is a key player in coordinating pyroptosis (Gu et al, 2022). Thus, miR-200b can induce pyroptosis in breast cancer cells by regulating the JAZF1/NF-κB axis.…”

Section: Noncoding Rnas and Pyroptosismentioning

confidence: 99%

Noncoding RNAs: the crucial role of programmed cell death in osteoporosis

Han,

Zhu,

Zhang

et al. 2024

Front. Cell Dev. Biol.

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Osteoporosis is the most common skeletal disease characterized by an imbalance between bone resorption and bone remodeling. Osteoporosis can lead to bone loss and bone microstructural deterioration. This increases the risk of bone fragility and fracture, severely reducing patients’ mobility and quality of life. However, the specific molecular mechanisms involved in the development of osteoporosis remain unclear. Increasing evidence suggests that multiple noncoding RNAs show differential expression in the osteoporosis state. Meanwhile, noncoding RNAs have been associated with an increased risk of osteoporosis and fracture. Noncoding RNAs are an important class of factors at the level of gene regulation and are mainly involved in cell proliferation, cell differentiation, and cell death. Programmed cell death is a genetically-regulated form of cell death involved in regulating the homeostasis of the internal environment. Noncoding RNA plays an important role in the programmed cell death process. The exploration of the noncoding RNA-programmed cell death axis has become an interesting area of research and has been shown to play a role in many diseases such as osteoporosis. In this review, we summarize the latest findings on the mechanism of noncoding RNA-mediated programmed cell death on bone homeostasis imbalance leading to osteoporosis. And we provide a deeper understanding of the role played by the noncoding RNA-programmed cell death axis at the gene regulatory level of osteoporosis. We hope to provide a unique opportunity to develop novel diagnostic and therapeutic approaches for osteoporosis.

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“…Upregulation of JAZF1 partially diminished the pro-pyroptotic effect of miR-200b. JAZF1 serves as a repressor of TAK1 that is a pivotal kinase upstream of the nuclear factor-κB (NF-κB) signaling cascade ( 121 ). Correspondingly, miR-200b overexpression and JAZF1 silencing caused the activation of the NF-κB signaling pathway ( 101 ).…”

Section: Noncoding Rna-mediated Pyroptosis In Cancermentioning

confidence: 99%

Noncoding RNA-mediated regulation of pyroptotic cell death in cancer

et al. 2022

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Pyroptosis is a newly discovered form of programmed cell death, which is manifested by DNA fragmentation, cell swelling, cell membrane rupture and leakage of cell contents. Previous studies have demonstrated that pyroptosis is tightly associated with the initiation and development of various cancers, whereas the molecular mechanisms underlying pyroptosis remain obscure. Noncoding RNAs (ncRNAs) are a type of heterogeneous transcripts that are broadly expressed in mammalian cells. Owing to their potency of regulating gene expression, ncRNAs play essential roles in physiological and pathological processes. NcRNAs are increasingly acknowledged as important regulators of the pyroptosis process. Importantly, the crosstalk between ncRNAs and pyroptosis affects various hallmarks of cancer, including cell growth, survival, metastasis and therapeutic resistance. The study of the involvement of pyroptosis-associated ncRNAs in cancer pathobiology has become a hot area in recent years, while there are limited reviews on this topic. Herein, we provide an overview of the complicated roles of ncRNAs, mainly including microRNAs (miRNAs), long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs), in modulating pyroptosis, with a focus on the underlying mechanisms of the ncRNA-pyroptosis axis in cancer pathogenesis. Finally, we discuss the potential applications and challenges of exploiting pyroptosis-regulating ncRNAs as molecular biomarkers and therapeutic targets in cancer.

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Preparation of a JAZF1 protein polyclonal antibody and its potential role in broiler ascites syndrome

Cited by 4 publications

References 30 publications

A Novel Rabbit Anti-Myoglobin Monoclonal Antibody’s Potential Application in Rhabdomyolysis Associated Acute Kidney Injury

A Novel Rabbit Anti-Myoglobin Monoclonal Antibody’s Potential Application in Rhabdomyolysis Associated Acute Kidney Injury

Noncoding RNAs: the crucial role of programmed cell death in osteoporosis

Noncoding RNA-mediated regulation of pyroptotic cell death in cancer

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