Preparation of a multi‐hollow magnetic molecularly imprinted polymer for the selective enrichment of indolebutyric acid

We describe a facile, general, and highly efficient approach to obtain polydopamine-coated molecularly imprinted polymer based on halloysite nanotubes for bovine serum albumin. The method combined surface molecular imprinting and one-step immobilized template technique. Hierarchically structured polymer was prepared in physiological conditions adopting dopamine as functional monomer. A thin layer of polydopamine can be coated on the surface of amino-modified halloysite nanotubes by self-polymerization, and the thickness of the imprinted shells can be controlled by the mass ratio of matrix and dopamine. The polymer was characterized by Fourier transform infrared spectrometry, transmission electron microscopy, and thermogravimetric analysis. The prepared material showed high binding capacity (45.4 mg/g) and specific recognition behavior toward the template protein. In addition, stability and regeneration analyses indicated that the imprinted polymer exhibited excellent reusability (relative standard deviation < 9% for batch-to-batch evaluation). Therefore, the developed polymer is effective for protein recognition and separation.

Section: Introductionmentioning

confidence: 99%

Halloysite‐based dopamine‐imprinted polymer for selective protein capture

Zhu

Liu

et al. 2016

“…The preparation of sieved monolithic MIPs presents several drawbacks such as the partial loss of binding sites due to polymer milling. Other more efficient ways of polymer imprinting have been developed . Nonetheless, monolith polymerization remains the simplest and fastest way to generate MIPs.…”

Section: Resultsmentioning

confidence: 99%

A two‐step strategy to visually identify molecularly imprinted polymers for tagged proteins

Brandis

Partouche

Yechezkel

et al. 2017

A practical and relatively simple method to identify molecularly imprinted polymers capable of binding proteins via the molecular tagging (epitope-like) approach has been developed. In our two-step method, we first challenge a previously obtained anti-tag molecularly imprinted polymer with a small molecule including the said tag of choice (a biotin derivative as shown here or other) connected to a linker bound to a second biotin moiety. An avidin molecule partially decorated with fluorescent labels is then allowed to bind the available biotin derivative associated with the polymer matrix. At the end of this simple process, and after washing off all the low-affinity binding molecules from the polymer matrix, only suitable molecularly imprinted polymers binding avidin through its previously acquired small molecule tag (or epitope-like probe, in a general case) will remain fluorescent. For confirmation, we tested the selective performance of the anti-biotin molecularly imprinted polymer binding it to biotinylated alkaline phosphatase. Residual chemical activity of the enzyme on the molecularly imprinted polymer solid support was observed. In all cases, the corresponding nonimprinted polymer controls were inactive.

“…After the polymerization and subsequent template removal, imprinted cavities are created that can provide sterically complementary voids and intermolecular forces for template‐specific rebinding . The separation of the target molecules is one of the most important applications for molecularly imprinted materials because these materials, which contain specific binding sites for the template molecules, offer strong potential for selective separation . An increasing number of studies focused on noncovalent imprinted polymers that could perform in the presence of water .…”

Section: Introductionmentioning

confidence: 99%

Recovery and separation of erythromycin from industrial wastewater by imprinted magnetic nanoparticles that exploit β‐cyclodextrin as the functional monomer

Zhang

Wang

et al. 2015

A type of surface imprinting over magnetic Fe3 O4 nanoparticles utilizing erythromycin-A as a template for use in the separation and recovery of erythromycin was developed and investigated. As the intermolecular forces play a key role in the performance of imprinted materials, differential scanning calorimetry, and (1) H NMR spectroscopy was employed to evaluate the interactions between erythromycin and the functional monomer β-cyclodextrin. To synthesize the surface imprinted polymers, magnetic Fe3 O4 nanoparticles, the core materials, were modified with a free radical initiator to initialize polymerization in a "grafting from" manner. Then using acryloyl-modified β-cyclodextrin as the functional monomer and ethyleneglycol dimethacrylate as the cross-linker, thin erythromycin-imprinted films were fabricated by the radical-induced graft copolymerization of monomers on the surface of the Fe3 O4 nanoparticles. Selectivity experiments showed that the erythromycin-A-imprinted materials had recognition ability toward erythromycin derivatives. Finally, these magnetic molecularly imprinted particles were successfully used for the separation and enrichment of erythromycin from the mother liquor. The recovery, detected by high-performance liquid chromatography and differential pulse voltammetry, approached 97%. The combination of the specific selectivity of the imprinted material and the magnetic separation provided a powerful tool that is simple, flexible, and selective for the separation and recovery of erythromycin.