Preparation of a multifunctional photoactivated prodrug on a streptavidin scaffold bearing a DNA aptamer

A detection system using sensing motif-tethered oligodeoxynucleotides for multiplex biomolecular analysis

“…The present system could detect a much lower target amount (GSH 4 pmol) than that of our previous method (GSH 400 pmol), which utilized disulfide bonds as a sensing unit for GSH. 11 Thus, we succeeded in improving the sensitivity by approximately 100 fold through the present method. In the present system, Mal-dsDNA1 easily reacts with the GSH in the solution.…”

“…7 Beyond the screening of ligand molecules of specific targets, the DNA encoding strategy has also been applied to detect biological targets. [8][9][10][11][12] For example, it has become possible to encode information on a vast number of individual cells for the analysis of the transcriptome at the single-cell level using the barcode sequence. 8 DNA tethered with antibodies or ligands enabled the simultaneous analysis of the transcriptome and multiple membrane surface biomolecules at a single-cell level.…”

“…9 Furthermore, target-responsive DNA probes have expanded the class of target biomolecules applicable to the DNA encoding strategy. [10][11][12] Among the systems previously reported, we designed a glutathione-responsive DNA probe labeled on streptavidin-coated magnetic beads to monitor endogenous GSH. 11 In addition, we managed to detect lactate and glucose.…”

“…[10][11][12] Among the systems previously reported, we designed a glutathione-responsive DNA probe labeled on streptavidin-coated magnetic beads to monitor endogenous GSH. 11 In addition, we managed to detect lactate and glucose. 12 The previous systems were useful for the analysis of biomolecules; however, simultaneous analysis using a DNA probe is difficult owing to the lack of a general strategy for designing DNA-based molecular probes for multiplex biomolecular analyses.…”

“…11 In addition, we managed to detect lactate and glucose. 12 The previous systems were useful for the analysis of biomolecules; however, simultaneous analysis using a DNA probe is difficult owing to the lack of a general strategy for designing DNA-based molecular probes for multiplex biomolecular analyses.…”