Preparation of a novel hybrid organic–inorganic monolith for the separation of lysozyme by high performance liquid chromatography

Bai, Ligai; Liu, Haiyan; Liu, Yankun; Zhang, Xinghua; Yang, Gengliang; Ma, Zhengyue

doi:10.1016/j.chroma.2010.10.115

Cited by 46 publications

(26 citation statements)

References 32 publications

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“…Compared with the traditional in situ synthetic technique of MIPs, hybrid organic-inorganic materials have significant advantages, such as good thermal stability, high hardness, excellent biocompatibility and good flexibility. 413 Most of the MISPE techniques usually couple with HPLC or GC for the determination of target analytes. Moreover, capillary electrophoresis (CE) is also an important and widely used separation tool owing to its advantages of high resolution, fast separation and small sample/reagent consumption.…”

Section: View Article Onlinementioning

confidence: 99%

Molecular imprinting: perspectives and applications

et al. 2016

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a Molecular imprinting technology (MIT), often described as a method of making a molecular lock to match a molecular key, is a technique for the creation of molecularly imprinted polymers (MIPs) with tailor-made binding sites complementary to the template molecules in shape, size and functional groups. Owing to their unique features of structure predictability, recognition specificity and application universality, MIPs have found a wide range of applications in various fields. Herein, we propose to comprehensively review the recent advances in molecular imprinting including versatile perspectives and applications, concerning novel preparation technologies and strategies of MIT, and highlight the applications of MIPs. The fundamentals of MIPs involving essential elements, preparation procedures and characterization methods are briefly outlined.Smart MIT for MIPs is especially highlighted including ingenious MIT (surface imprinting, nanoimprinting, etc.), special strategies of MIT (dummy imprinting, segment imprinting, etc.) and stimuli-responsive MIT (single/dual/ multi-responsive technology). By virtue of smart MIT, new formatted MIPs gain popularity for versatile applications, including sample pretreatment/chromatographic separation (solid phase extraction, monolithic column chromatography, etc.) and chemical/biological sensing (electrochemical sensing, fluorescence sensing, etc.). Finally, we propose the remaining challenges and future perspectives to accelerate the development of MIT, and to utilize it for further developing versatile MIPs with a wide range of applications (650 references).

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Section: View Article Onlinementioning

confidence: 99%

Molecular imprinting: perspectives and applications

et al. 2016

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“…Hybrid organic-inorganic monoliths consist of two or more integrating components combined at the molecular or nanometer level, and have many advantages, such as flexibility, low column backpressure, and long life with excellent biocompatibility and mechanical properties (30). Moreover, hybrid materials can be tailored to exhibit desirable properties for comprehensive applications (31,32), because they are simple to process and are amenable to design on the molecular scale.…”

Section: Introductionmentioning

confidence: 99%

Extraction of Astaxanthin from Shrimp Waste using Response Surface Methodology and a New Hybrid Organic-Inorganic Monolith

Zhu

Row

2013

Separation Science and Technology

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A 17-run Box-Behnken design (BBD) with three factors was used to improve the conditions for extracting astaxanthin from shrimp waste. The astaxanthin level was determined by high performance liquid chromatography (HPLC) using a C 18 column and a UV detector at 476 nm. The mean extraction yield of astaxanthin at the improved conditions (extraction time 1.4 h, extraction temperature 72.4 C, and liquid-solid ratio 27.3) was 98.7 AE 2.6 lg g À1 . A solid-phase extraction (SPE)-HPLC method was developed with a new hybrid organic-inorganic hybrid monolith for further purification of astaxanthin from shrimp waste. The SPE recoveries were ranging from 81.3 AE 2.4% to 86.5 AE 3.3%, and the intra-day and inter-day relative standard deviations (RSDs) of the proposed method were less than 4.3 AE 0.5% and 4.8 AE 0.2%, respectively. BBD increased the extraction efficiency and shortened extraction times significantly. SPE-HPLC with hybrid monolith showed good selectivity and purified astaxanthin from shrimp waste.

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“…Besides, by replacing the traditional free-radical polymerization with other polymerization reactions, such as living radical polymerization [10] and ring-opening metathesis polymerization [11,12], the microstructure homogeneity of hybrid monolith could be remarkably improved, achieving better chromatographic performance in capillary liquid chromatography (cLC). However, a great deal of limitations, such as harsh reaction conditions, tedious experimental operation and few available monomers, greatly restricted their application in preparation of hybrid monolith with homogenous microstructure [13].…”

Section: Introductionmentioning

confidence: 99%