Preparation of a Silk Fibroin Spongy Wound Dressing and Its Therapeutic Efficiency in Skin Defects

Min, Sijia; Gao, Xin; Han, Chunmao; Chen, Yu; Yang, Mingying; Zhu, Liang; Zhang, Haiping; Liu, Lin; Yao, Juming

doi:10.1163/092050610x543609

Cited by 51 publications

(23 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The water-absorbing capacity of the scaffolds was measured according to a previous method60. Briefly, the dried scaffolds were cut into Φ10 × 5 mm cylinders and weighed (W 1 ).…”

Section: Methodsmentioning

confidence: 99%

Enhanced osteogenesis and angiogenesis by mesoporous hydroxyapatite microspheres-derived simvastatin sustained release system for superior bone regeneration

Sun

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Biomaterials with both excellent osteogenic and angiogenic activities are desirable to repair massive bone defects. In this study, simvastatin with both osteogenic and angiogenic activities was incorporated into the mesoporous hydroxyapatite microspheres (MHMs) synthesized through a microwave-assisted hydrothermal method using fructose 1,6-bisphosphate trisodium salt (FBP) as an organic phosphorous source. The effects of the simvastatin-loaded MHMs (S-MHMs) on the osteogenic differentiation of rat bone marrow mesenchymal stem cells (rBMSCs) and angiogenesis in EA.hy926 cells were investigated. The results showed that the S-MHMs not only enhanced the expression of osteogenic markers in rBMSCs but also promoted the migration and tube formation of EA.hy926 cells. Furthermore, the S-MHMs were incorporated into collagen matrix to construct a novel S-MHMs/collagen composite scaffold. With the aid of MHMs, the water-insoluble simvastatin was homogenously incorporated into the hydrophilic collagen matrix and presented a sustained release profile. In vivo experiments showed that the S-MHMs/collagen scaffolds enhanced the bone regeneration and neovascularization simultaneously. These results demonstrated that the water-insoluble simvastatin could be incorporated into the MHMs and maintained its biological activities, more importantly, the S-MHMs/collagen scaffolds fabricated in this study are of immense potential in bone defect repair by enhancing osteogenesis and angiogenesis simultaneously.

show abstract

“…The water-absorbing capacity of the scaffolds was measured according to a previous method60. Briefly, the dried scaffolds were cut into Φ10 × 5 mm cylinders and weighed (W 1 ).…”

Section: Methodsmentioning

confidence: 99%

Enhanced osteogenesis and angiogenesis by mesoporous hydroxyapatite microspheres-derived simvastatin sustained release system for superior bone regeneration

Sun

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…In the latter case, SF might be an interesting choice and in light of its excellent stabilization potential for protein drugs after implantation [40, 41], such that sustained delivery profiles even for sensitive drugs may become feasible by this painless approach. Studies using SF as dermal fillers would suggest such intradermally placed needles and, thereby, the sustained presence within the skin will be biocompatible [42]. Release kinetics from such SF needles may be tailored by post-processing treatment as a function of system crystallinity, as has been demonstrated for SF particles, films or scaffolds for enzymes, growth factors and other drugs, respectively [32, 35, 43-45].…”

Section: Generalized Musculoskeletal Diseases – How Can Sf Contributementioning

confidence: 99%

Silk constructs for delivery of musculoskeletal therapeutics

Meinel¹,

Kaplan²

2012

Advanced Drug Delivery Reviews

101

View full text Add to dashboard Cite

Silk fibroin (SF) is a biopolymer with distinguishing features from many other bio- as well as synthetic polymers. From a biomechanical and drug delivery perspective, SF combines remarkable versatility for scaffolding (solid implants, hydrogels, threads, solutions), with advanced mechanical properties and good stabilization and controlled delivery of entrapped protein and small molecule drugs, respectively. It is this combination of mechanical and pharmaceutical features which render SF so exciting for biomedical applications. his pattern along with the versatility of this biopolymer have been translated into progress for musculoskeletal applications. We review the use and potential of silk fibroin for systemic and localized delivery of therapeutics in diseases affecting the musculoskeletal system. We also present future directions for this biopolymer as well as the necessary research and development steps for their achievement.

show abstract

“…In vitro studies indicate that cells such as fibroblasts are able to proliferate and express extracellular matrix [122], and the in vivo results show revascularization of the silk graft [122], good cell colonization [115], and no signs of acute dermal toxicity [123]. In vitro studies indicate that cells such as fibroblasts are able to proliferate and express extracellular matrix [122], and the in vivo results show revascularization of the silk graft [122], good cell colonization [115], and no signs of acute dermal toxicity [123].…”

Section: Biomedical Applications Of Silkmentioning

confidence: 99%

Silk‐Based Biomaterials

Gomes¹,

Leonor²,

Mano³

et al. 2012

Biomimetic Approaches for Biomaterials Development

View full text Add to dashboard Cite

IntroductionSilk is a hierarchically structured fibrous protein that has been used for thousands of years for different applications such as textile production and wound dressings [1]. Although silk proteins are produced by a large number of arthropod species such as wasps, bees, and crickets, during the past few decades, the scientific community has focused its attention in the silk produced by the mulberry silkworm Bombyx mori and by different spider species. Farming of the silkworm B. mori started around 5000 years ago in China, and since then, the cocoon case produced by this animal has been used as a source of silk for the textile industry. More recently, B. mori silk has been used commercially in the development of sutures for medical applications [1,2]. In contrast to B. mori silk, spider silk has not been commercialized for biomedical proposes mainly because of the territorial cannibalistic behavior of spiders, which makes them difficult to farm, along with the low levels of silk production. However, in the past, spider webs have been used as bandages for wound dressing, because of its antimicrobial properties, and in other human activities such as fishing and hunting [3]. The results from recent studies demonstrating the biocompatibility of silk and the exquisite mechanical properties of silk fibers, especially in the case of spider silk, are the main reasons why B. mori and spider silk proteins have been the target of intense research efforts, when compared with the silk produced by other arthropods, aimed at developing new silk-based biomaterials with superior mechanical and biological properties.On the basis of the background given above and since B. mori and spider silk are the most extensively characterized silk, this chapter focuses on the silk proteins produced by these animals and on the perspectives of using these proteins for future biomedical applications.The silk produced by B. mori is the most widely used, since this silkworm is easy to farm, allowing for steady and large-scale production [1]. The B. mori silk fiber is formed by two microfilaments embedded in a gluelike glycoprotein named sericin, which works as a coating and is used by the animal for cocoon assembly. Each microfilament results from the assembly of a hydrophobic ∼370 kDa heavy-chain fibroin protein, with a relatively hydrophilic ∼25 kDa light-chain fibroin and a 30 kDa P25 protein [4]. The heavy-chain fibroin is a large insoluble macromolecule formed by ∼5263 amino acids and is rich in glycine (G, 45.9%), alanine (A, 30.3%), serine (S, 12.2%), tyrosine (Y, 5.3%), and valine (V, 1.8%) residues [1,5]. This heavy-chain fibroin is formed by a highly repetitive crystalline fraction of 2377 repeats of GX. The X position is occupied by the A residue in 64% of the repeats, by S in 22%, by Y in 10%, by V in 3%, and by threonine (T) in 1.3% of the repeats [5,6]. These repetitive cores form 12 domains that alternate with 11 nonrepetitive amorphous domains formed by 25 amino acids residues, which connect the crystalline domains. These a...

show abstract

Preparation of a Silk Fibroin Spongy Wound Dressing and Its Therapeutic Efficiency in Skin Defects

Cited by 51 publications

References 26 publications

Enhanced osteogenesis and angiogenesis by mesoporous hydroxyapatite microspheres-derived simvastatin sustained release system for superior bone regeneration

Enhanced osteogenesis and angiogenesis by mesoporous hydroxyapatite microspheres-derived simvastatin sustained release system for superior bone regeneration

Silk constructs for delivery of musculoskeletal therapeutics

Silk‐Based Biomaterials

Contact Info

Product

Resources

About