Preparation of Active Chitooligosaccharides with a Novel Chitosanase <i>Aq</i>CoA and Their Application in Fungal Disease Protection

Wang, Yanxin; Ding, Li; Liu, Muxing; Xia, Chengyao; Fan, Qi‐Wen; Xu, Lixin; Lan, Zejun; Shi, Guolong; Dong, Weiliang; Li, Zhoukun; Cui, Zhongli

doi:10.1021/acs.jafc.0c07802

Cited by 28 publications

(12 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The EC 50 of M. oryzae and C. higginsianum with COSs-Csn treatment were 4.84 and 8.47 mg/mL, respectively, while the values with COSs-SGY treatment were 6.06 and 9.91 mg/mL, respectively. These results were consistent with previous study showing that COSs exhibited good antifungal activity (Wang et al, 2021b), because the cationic -NH 2 groups of COSs can form ammonium groups by absorbing H + , and then bind to negatively charged components of the microbial cell wall, resulting in agminated microbes and further lysis (Liaqat and Eltem, 2018).…”

Section: Antifungal Activity Of Cosssupporting

confidence: 93%

“…Among them, DP ≥ 5 was essential for antimicrobial activity of fully deacetylated COS (Li et al, 2014). The growth inhibition rate (27.35%) of M. oryzae with 4 mg/mL COSs-Csn treatment (Figure 5A) was higher than CoA-COSs (20.0%) in a previous study (Wang et al, 2021b); the reason may be related to the difference of DP. COSs-Csn was composed of DP2-6, whereas CoA-COSs consisted of DP3-5.…”

Section: Antifungal Activity Of Cossmentioning

confidence: 74%

“…Many enzymes have been reported to hydrolyze chitosan, such as cellulase, papain, pepsin, pectinase, and lipase, and chitosanase (EC. 3.2.1.132) hydrolyzes chitosan specifically (Wang et al, 2021b).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Efficient Preparation of Chitooligosaccharide With a Potential Chitosanase Csn-SH and Its Application for Fungi Disease Protection

Cui

Yang

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

Chitosanase plays a vital role in bioactive chitooligosaccharide preparation. Here, we characterized and prepared a potential GH46 family chitosanase from Bacillus atrophaeus BSS. The purified recombinant enzyme Csn-SH showed a molecular weight of 27.0 kDa. Csn-SH displayed maximal activity toward chitosan at pH 5.0 and 45°C. Thin-layer chromatography and electrospray ionization–mass spectrometry indicated that Csn-SH mainly hydrolyzed chitosan into (GlcN)2, (GlcN)3, and (GlcN)4 with an endo-type cleavage pattern. Molecular docking analysis demonstrated that Csn-SH cleaved the glycoside bonds between subsites −2 and + 1 of (GlcN)6. Importantly, the chitosan hydrolysis rate of Csn-SH reached 80.57% within 40 min, which could reduce time and water consumption. The hydrolysates prepared with Csn-SH exhibited a good antifungal activity against Magnaporthe oryzae and Colletotrichum higginsianum. The above results suggested that Csn-SH could be used to produce active chitooligosaccharides efficiently that are biocontrol agents applicable for safe and sustainable agricultural production.

show abstract

Section: Antifungal Activity Of Cosssupporting

confidence: 93%

Section: Antifungal Activity Of Cossmentioning

confidence: 74%

See 1 more Smart Citation

Efficient Preparation of Chitooligosaccharide With a Potential Chitosanase Csn-SH and Its Application for Fungi Disease Protection

Cui

Yang

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Co39) to assess the plant response. The culture of rice, extraction of RNA, and determination of expression levels of the plant resistance genes were performed as described by Wang et al The stable expression OsActin (ABF98567.1) amplified by primer pairs Os Actin-QF/ Os Actin-QR (Table ) was used as an internal control. The expression levels of pathogenesis-related (PR) genes (pathogenesis-related protein 1a ( PR1a ) (AJ278436.1), probenazole-inducible protein ( PBZ1 ) (D38170.1), and chitinase ( CHT1 ) (XM_015788082)) were detected by the primer pairs (PR1a-QF/PR1a-QR, PBZ1-QF/PBZ1-QR, and CHT1-QF/CHT1-QR).…”

Section: Methodsmentioning

confidence: 99%

Functional Characterization of the Novel Laminaripentaose-Producing β-1,3-Glucanase MoGluB and Its Biocontrol of Magnaporthe oryzae

Wang

Zhao

Wang

et al. 2021

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

Fungal cell wall synthesizing enzymes or remodeling enzymes represent key factors for the interaction of plant pathogen and antifungal agents, which are regarded as potential biocontrol agents. In this study, a novel endo-β-1,3-glucanase from Magnaporthe oryzae was expressed and characterized. The expression of MoGluB was significantly upregulated after 2 days of liquid culture and 48 h after infection, indicating that it may be involved in cell wall reconstitution. Purified MoGluB exhibited high activity on insoluble β-glucans, with a specific activity of 8.18 U/mg toward yeast glucan at pH 9.0 and 50 °C. MoGluB hydrolyzed pachymaran and yeast glucan into oligosaccharides dominated by laminaripentaose, suggesting that it is an endo-β-1,3-glucanase. Incubation of 8 μg of MoGluB with 106 spores/mL resulted in the inhibition of conidial germination and appressorium formation of M. oryzae, illustrating effective biocontrol activity. Hydrolysates of pachymaran induced the expression of defense genes restricting M. oryzae infection in rice plants, indicating an immunostimulatory effect of MoGluB hydrolysates.

show abstract

“…V max (µM min −1 ml −1 ) 959 sodium carboxymethylcellulose and β-glucan (Jiang et al, 2021;Wang Y. X. et al, 2021).…”

Section: Kinetic Parameters Valuementioning

confidence: 99%

Overexpression and Biochemical Properties of a GH46 Chitosanase From Marine Streptomyces hygroscopicus R1 Suitable for Chitosan Oligosaccharides Preparation

Wang

Wang²,

Zhu³

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

Due to the various biological activities of chitosan oligosaccharides (COSs), they have great potential value for use in many areas. Chitosanase plays an important role in enzymatic preparation of COSs. Herein, a gene encoding a chitosanase (ShCsn46) from marine Streptomyces hygroscopicus R1 was cloned and the sequences encoding ShCsn46 without signal peptide were optimized based on the codon usage of Pichia pastoris (P. pastoris). In addition, the optimized gene was ligated to pPICZαA and transformed to P. pastoris X33. After screening, a recombinant strain named X33-Sh33 with the highest activity was isolated from 96 recombinant colonies. The maximum activity and total protein concentration of the recombinant strain ShCsn46 were 2250 U/ml and 3.98 g/l, respectively. The optimal pH and temperature of purified ShCsn46 were 5.5 and 55°C, respectively. Meanwhile, ShCsn46 was stable from pH 5.0 to 10.0 and 40 to 55°C, respectively. The purified ShCsn46 was activated by Mn2+ and inhibited by Cu2+, Fe2+, and Al3+. In addition, substrate specificity of the purified ShCsn46 showed highest activity toward colloidal chitosan with 95% degree of deacetylation. Furthermore, the purified ShCsn46 exhibited high efficiency to hydrolyze 4% colloidal chitosan to prepare COSs. COSs with degree of polymerization of 2–6, 2–5, and 2–4 were controllably produced by adjusting the reaction time. This study provides an excellent chitosanase for the controllable preparation of COSs with a desirable degree of polymerization.

show abstract

Preparation of Active Chitooligosaccharides with a Novel Chitosanase AqCoA and Their Application in Fungal Disease Protection

Cited by 28 publications

References 55 publications

Efficient Preparation of Chitooligosaccharide With a Potential Chitosanase Csn-SH and Its Application for Fungi Disease Protection

Efficient Preparation of Chitooligosaccharide With a Potential Chitosanase Csn-SH and Its Application for Fungi Disease Protection

Functional Characterization of the Novel Laminaripentaose-Producing β-1,3-Glucanase MoGluB and Its Biocontrol of Magnaporthe oryzae

Overexpression and Biochemical Properties of a GH46 Chitosanase From Marine Streptomyces hygroscopicus R1 Suitable for Chitosan Oligosaccharides Preparation

Contact Info

Product

Resources

About