Preparation of an electrochemical immunosensor based on a Cu/Cu₂O-rGO@Au signal synergistic amplification strategy and efficient and sensitive detection of alpha-fetoprotein

Abstract

The effective amplification of the signal is the prerequisite for the ultrasensitive detection of electrochemical immunosensors. To quantitatively detect alpha-fetoprotein (AFP), we prepared a sandwich-type electrochemical immunosensor. Using gold nanoparticles...

Recent advances on the development of graphene‐based field‐effect transistors, electrochemical biosensors and electrochemiluminescence biosensors

“…The typical sandwich‐type electrochemical immunosensors can be constructed through label of the specific detection antibody on graphene‐based nanomaterials, the representative fabrication process was illustrated in Figure 7a. By applying the nitrogen‐doped graphene‐based immunosensors, the sensitive detection of neuron‐specific enolase [88], alpha‐fetoprotein [87a, 89], carbohydrate antigen 125 [87a], prostate specific antigen [87a] were accomplished. In addition, after the ionic liquids [90], Au nanoparticles [87b, 91], Pd/Pt/MoS 2 [92] and ZnMn 2 O 4 [93] were functionalized on graphene substrate, the enhanced loading capacity of the capture antibody or the transport of electron can further promote the detection performance of the immunosensors on the biomarkers like cluster of differentiation‐44 antigen, carbohydrate antigen 15–3, alpha‐fetoprotein and carcinoembryonic antigen.…”

Construction of a Dual-Mode Immune Platform Based on the Photothermal Effect of AgCo@NC NPs for the Detection of α-Fetoprotein

“…The construction of the electrochemical immunization platform is described in Scheme (C). The specific process is referred to the literature. , In brief, sodium alginate solution (SA, 0.2 wt %) was added dropwise to the surface of the glassy carbon electrode (GCE) electrode for adsorption of MB, followed by 10 μL of Au-rGO for coupling Ab 1 ,12 μL of Ab 1 , 8 μL of BSA, and 12 μL of AFP antigen at different concentrations were then added dropwise to the electrode surface for incubation, and finally after the high selectivity of the antigen and antibody was completed, 20 μL of the mixed solution (containing 30 mM H 2 O 2 , 0.4 mM ACAC, 0.25 M NIPAM, and 2.5 mM MBA) was added dropwise to the electrode, irradiated under NIR light for 2 min, and then placed at room temperature for 1 h. The SWV test was then performed.…”

“…The specific process is referred to the literature. 24,25 In brief, sodium alginate solution (SA, 0.2 wt %) was added dropwise to the surface of the glassy carbon electrode (GCE) electrode for adsorption of MB, followed by 10 μL of Au-rGO for coupling Ab 1 ,12 μL of Ab 1 , 8 μL of BSA, and 12 μL of AFP antigen at different concentrations were then added dropwise to the electrode surface for incubation, and finally after the high selectivity of the antigen and antibody was completed, 20 μL of the mixed solution (containing 30 mM H 2 O 2 , 0.4 mM ACAC, 0.25 M NIPAM, and 2.5 mM MBA) was added dropwise to the electrode, irradiated under NIR light for 2 min, and then placed at room temperature for 1 h. The SWV test was then performed. To guarantee the accuracy of the immunosensor test, we first tested the assembled electrodes in 20 mL of a solution containing 5 mM [Fe(CN) 6 ] 4−/3− and 0.1 M KCl for cyclic voltammetry (CV) and electrochemical impedance (EIS) to check whether the electrode assembly was successful.…”