Preparation of an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine-silica hybrid monolithic column for the enantioseparation of amino acids by nano-liquid chromatography

Review of recent advances in development and applications of organic‐silica hybrid monoliths

“…The selection of the organic monomer dictates the separation mode and potential applications (Table 2). Such is an example of Gemini‐type sulfobetaine‐based [48] and O ‐[2‐(methacryloyloxy)‐ethylcarbamoyl]−10,11‐dihydroquinidine (MQD)‐based [49] monoliths prepared using acid‐catalyzed sol‐gel reaction of TMOS and VTMS or triethoxyvinylsilane (VTES) and free‐radical polymerization with corresponding organic monomers. Sulfobetaine zwitterionic Gemini functional groups aided the separation of small polar analytes such as nucleosides, phenols, amides, bases, and bovine serum albumin (BSA) tryptic digest in HILIC mode.…”

“…A plate height of 10 μm was measured for thiourea, which is lower than that reported for hybrid silica capillaries [13]. The chiral MQD‐based column was evaluated for enantioseparation of N‐derivatized amino acids under reversed‐phase and polar organic‐phase mode [49]. Even though interesting organic monomers were selected leading to unusual surface chemistries, both monomers need to be synthesized in‐house, which adds extra steps to the otherwise simple one‐pot procedure.…”

Chiral Monolithic Silica-Based HPLC Columns for Enantiomeric Separation and Determination: Functionalization of Chiral Selector and Recognition of Selector-Selectand Interaction

“…Likewise, Pittler & Schmid [88] carried out the enantioseparation of dansyl amino acids by HPLC on a monolithic column dynamically coated with a vancomycin derivative. Xu et al [89], prepared an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine (MQD)-silica hybrid monolithic column, which was applied for the chiral analysis of 52 N-derivatized protein and non-protein amino acids. Accordingly, the same research group, achieved the enantioseparation of L-norvaline and L-tryptophan in dietary supplements using an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine-silica hybrid monolithic column [90].…”

Influence of photo-initiators in the preparation of methacrylate monoliths into poly(ethylene-co-tetrafluoroethylene) tubing for microbore HPLC

“…In recent decades, the number of analytical applications of organic polymer-based monoliths has grown progressively, covering innovations chromatography and sample preparation fields [1][2][3]. The key to success lies in their simple preparation, high permeability, tunable properties, and easily available surface chemistries.…”