Preparation of antibodies and development of enzyme-linked immunosorbent assay for nonylphenol

Мартьянов, А. А.; Zherdev, Anatoly V.; Eremin, Sergei A.; Dzantiev, Boris B.

doi:10.1080/03067310410001729024

Cited by 19 publications

(17 citation statements)

References 44 publications

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“…The hydrophobicity of the molecule (log Pow 4.48, ref 17) determines its low water solubility (5 mg L -1 ) and the tendency of this substance to adsorb to the solid surfaces (i.e., plasticware). Despite the immunochemical techniques developed for NP (ELISAs (14)(15)(16)18), dipstick assays (19), polarization fluoroimmunoassays (20,21), flow injection immunoassays (22), and capillary immunoassays ( 23)), this issue has never been seriously addressed.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Nonylphenol: Advances and Improvements in the Immunochemical Determination Using Antibodies Raised against the Technical Mixture and Hydrophilic Immunoreagents

Estévez

Kreuzer

Sánchez‐Baeza

et al. 2005

Environ. Sci. Technol.

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The development of an enzyme-linked immunosorbent assay (ELISA) for the detection of technical nonylphenol (NP) is reported. The preparation of specific antibodies has been addressed using an immunizing hapten with a four-carbon atom spacer arm placed at the ortho position that preserves both the hydroxyl group and the complexity of the branched nonyl chain mixture of the technical NP. The synthesis of the immunizing hapten 5-(2-hydroxy-5-nonylphenyl)-pentanoic acid has been accomplished through a four-step synthetic pathway using the NP commercial technical mixture as the starting material. Three types of competitor haptens have also been prepared depending on the location of the spacer arm: in ortho position to the phenol group (type A), attached to the oxygen atom (type B), and in para position, substituting the nonyl chain (type C). Drawbacks produced by the hydrophobicity of the NP or of the hapten derivatives have been circumvented by using a highly hydrophilic carrier molecule such as a high-molecular-weight aminodextran as a coating support for antigen in an indirect ELISA format. A reproducible and sensitive indirect competitive ELISA has been finally obtained, reaching a limit of detection of 2.3 +/- 0.9 microg L(-1) and an IC50 value of 29 +/- 5 microg L(-1) (both N = 16). A coefficient of variation of 11% for assays performed on different days (N = 5; IC50 = 30 +/- 3 microg L(-1)) demonstrates the assay reproducibility. The assay also recognizes the nonylphenol polyethoxylates to a different degree depending on the length of the ethoxylate chain. Recovery values in the range between 96 and 100% have been obtained using spiked blind aqueous samples although the sample preparation procedure used has been shown to have a great influence on the method accuracy. A preliminary evaluation of the analytical protocol established has been performed using real water samples.

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Section: Introductionmentioning

confidence: 99%

Analysis of Nonylphenol: Advances and Improvements in the Immunochemical Determination Using Antibodies Raised against the Technical Mixture and Hydrophilic Immunoreagents

Estévez

Kreuzer

Sánchez‐Baeza

et al. 2005

Environ. Sci. Technol.

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“…The synthesis of OP1 was carried out according to the NP study reported by Estévez et al (2006). In their study, the NP hapten with a linker on the ortho-position of the phenol group was used as an immunogen, and the antibodies raised yielded better sensitivity than in the other studies in which the linkers were attached on the alkyl chain or the hydroxyl group (Goda et al 2000;Fránek, et al 2001;Mart'ianov et al 2004). …”

Section: Resultsmentioning

confidence: 99%

“…There are a few immunoassays with broad specificity reported for the detection of alkylphenol polyethoxylates, nonyphenols, and OP (Estévez-Alberola and Marco 2004;Fránek, et al 2001;Goda et al 2000;Mart'ianov et al 2004;Rose et al 2002). The antibodies raised against haptens synthesized using technical OP products, which contain a large number of isomers with structural diversity, therefore showed broad specificity.…”

Section: Introductionmentioning

confidence: 98%

Development of a Competitive ELISA for the Detection of 4-tert-Octylphenol in Seafood

et al. 2015

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The rational hapten design and development of an indirect enzyme-linked immunosorbent assay (ELISA) for the detection of 4-tert-octylphenol (4-tert-OP), an endocrinedisrupting compound belonging to alkylphenols (APs), is described. The polyclonal antibody highly specific to 4-tert-OP was raised against a hapten with a linker attached to the ortho-position of the phenol ring. Using 5-(2-hydroxy-5-nonylphenyl)-5-oxo-pentanoic acid (NP1) as a competing hapten, an indirect competitive assay exhibiting an IC 50 value of 100 μg L −1 and the limit of detection (LOD) of 10 μg L −1 was developed. The assay was highly specific to 4-tert-OP and only displayed a moderate cross-reactivity (29 %) with IGEPAL® CA-520. The immunodominance was suggested to be both the 4-tert-octyl chain and the phenol group. The assay was optimized for the analysis of 4-tert-OP in prawn and clam samples with the LOD of 20 μg L −1 and the % recovery of 80-120 %.

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“…An enzyme-linked immunosorbent assay (ELISA) was carried out to detect the estrogen activity of samples according to previous studies. , Samples and the standard were reacted with the detection antibody for 1 h. After the washing solution was removed, the substrate was added and incubated for 15 min in the dark. The corresponding optical density (OD) value was measured after the reaction was terminated.…”

Section: Methodsmentioning

confidence: 99%

Novel Electrochemical Pretreatment for Preferential Removal of Nonylphenol in Industrial Wastewater: Biodegradability Improvement and Toxicity Reduction

Niu

Cai

Song

et al. 2019

Environ. Sci. Technol.

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Preferential pretreatment of nonylphenol (NP) before biological treatment is of great significance due to its horizontal gene transfer effect and endocrine disruption activity. A novel molecular imprinting high-index facet SnO 2 (MI-SnO 2, HIF ) electrode is designed. NP was effectively removed from industrial wastewater at 1.8 V with totally suppressing human estrogen activity. The ratio of 5 day biological oxygen demand to chemical oxygen demand (BOD 5 /COD Cr ) was enhanced to 0.412 from 0.186 after preferential pretreatment. The effluent concentration of NP was 6.4 μg L −1 after further simulating anaerobic-anoxic-oxic treatment, which was about 1/10 of that without pretreatment. This preferential electrochemical pretreatment is interpreted as prior adsorption and enrichment of target pollutants on the MI-SnO 2, HIF surface. The reactive oxygen species and subsequent oxidation products were investigated by in situ electron paramagnetic resonance and electrochemical infrared spectroscopy. The degradation pathway of NP was further analyzed by liquid chromatography−mass spectrometry. This unique pretreatment method for a complex tannery wastewater system has irreplaceable status because no methods with similar advantages have been reported, expecting to be widely used in preferential pretreatment of toxic contaminants blended with highly concentrated nontoxic organics.

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Preparation of antibodies and development of enzyme-linked immunosorbent assay for nonylphenol

Cited by 19 publications

References 44 publications

Analysis of Nonylphenol: Advances and Improvements in the Immunochemical Determination Using Antibodies Raised against the Technical Mixture and Hydrophilic Immunoreagents

Analysis of Nonylphenol: Advances and Improvements in the Immunochemical Determination Using Antibodies Raised against the Technical Mixture and Hydrophilic Immunoreagents

Development of a Competitive ELISA for the Detection of 4-tert-Octylphenol in Seafood

Novel Electrochemical Pretreatment for Preferential Removal of Nonylphenol in Industrial Wastewater: Biodegradability Improvement and Toxicity Reduction

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