Preparation of Antioxidant Protein Hydrolysates from Pleurotus geesteranus and Their Protective Effects on H2O2 Oxidative Damaged PC12 Cells

Abstract

Pleurotus geesteranus is a promising source of bioactive compounds. However, knowledge of the antioxidant behaviors of P. geesteranus protein hydrolysates (PGPHs) is limited. In this study, PGPHs were prepared with papain, alcalase, flavourzyme, pepsin, and pancreatin, respectively. The antioxidant properties and cytoprotective effects against oxidative stress of PGPHs were investigated using different chemical assays and H2O2 damaged PC12 cells, respectively. The results showed that PGPHs exhibited superior antioxidant activity. Especially, hydrolysate generated by alcalase displayed the strongest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (91.62%), 2,2-azino-bis (3-ethylbenzothia zoline-6-sulfonic acid) (ABTS) radical scavenging activity (90.53%), ferric reducing antioxidant power, and metal ion-chelating activity (82.16%). Analysis of amino acid composition revealed that this hydrolysate was rich in hydrophobic, negatively charged, and aromatic amino acids, contributing to its superior antioxidant properties. Additionally, alcalase hydrolysate showed cytoprotective effects on H2O2-induced oxidative stress in PC12 cells via diminishing intracellular reactive oxygen species (ROS) accumulation by stimulating antioxidant enzyme activities. Taken together, alcalase hydrolysate of P. geesteranus protein can be used as beneficial ingredients with antioxidant properties and protective effects against ROS-mediated oxidative stress.

Effect of clover sprouts protein hydrolysates as an egg substitute on physicochemical and sensory properties of mayonnaise

“…Liao et al. (2020) stated that the total HAA of the hydrolyzed protein of the fungus ( Pleurotus geesteranus ) by alcalase and flavourzyme enzymes were 40.66% and 30.96%, respectively.…”

Protein hydrolysates from Pleurotus geesteranus obtained by simulated gastrointestinal digestion exhibit neuroprotective effects in H 2 O 2 ‐injured PC12 cells

“…The bioactivity of the peptides within the hydrolysates is related to the amino acids, and the type and amount of charges available (Liao et al., 2020; Wu et al., 2021). Therefore, we determined the composition and content of the amino acids within the hydrolysates (Table 2).…”

“…The expression levels of cat , GPx , sod1 , and sod2 were significantly increased in the H 2 O 2 ‐injured cells pre‐treated with hydrolysates in the present study (Figure 5d). The hydrolysates, obtained from P. geesteranus (Liao et al., 2020) and Decapterus maruadsi (Zhang et al., 2020), could also significantly exhibit antioxidant properties and neuroprotection in oxidatively damaged PC12 cells. Therefore, protein hydrolysates from P. geesteranus may play a role not only as an exogenous antioxidant against oxidative stress by scavenging ROS (Figure 4b,c), but also as a modulator to enhance the expression of endogenous antioxidant enzymes (Figure 5d).…”

“…The ROS level showed no significant difference between the PC12 cells without any treatment and those that underwent pre-treatment with the hydrolysates (Figure 4a). Upon The bioactivity of the peptides within the hydrolysates is related to the amino acids, and the type and amount of charges available (Liao et al, 2020;Wu et al, 2021). Therefore, we determined the composition and content of the amino acids within the hydrolysates (Table 2).…”

“…The expression levels of cat, GPx, sod1, and sod2 were significantly increased in the H 2 O 2 -injured cells pre-treated with hydrolysates in the present study (Figure5d). The hydrolysates, obtained from P. geesteranus(Liao et al, 2020) and Decapterus maruadsi(Zhang et al, 2020), couldF I G U R E 5 The activity and gene expression of antioxidant enzymes under different treatments. (a-c) Changes in (a) CAT, (b) GPx, and (c) SOD under different treatments.…”