Preparation of aqueous gel beads coated by lipid bilayers

Campbell, Andrew L.; Taylor, Pietro; Cayre, Olivier J.; Paunov, Vesselin N.

doi:10.1039/b406941b

Cited by 17 publications

(19 citation statements)

References 12 publications

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“…Thermal and ionotropic cross-linking are the examples of physical cross-linking reactions. Some hydrogel cores were made of polysaccharides when temperature changes [2,5,32]. Indeed, agarose [5] and κ-carrageenan [32] are the temperature-sensitive polysaccharides which structure in aqueous solutions undergoes a transition from a random-coil conformation to the cross-linked double helixes upon cooling.…”

Section: Liposomes 54mentioning

confidence: 99%

“…For 100-nm lipobeads, the liposomal formulations have to be sonicated [1,9] or extruded through a nanopore filter of a needed pore size [5,8,11,14,16,19,32]. Another approach to control the size of pre-lipobeads is based on hydrodynamic focusing of a stream of the liposome precursor solution by the flow of a hydrogel-forming solution within a microfluidic device [21].…”

Section: Polymerization Within Liposomal Nano-/microreactorsmentioning

confidence: 99%

“…First of all, encapsulation of hydrogel-forming components into liposomes can result from hydration of lipid cast film formed upon solvent evaporation [1,2,5,9,11,13,14,16,32], electroformation [12,22,33,34], or rapid phase evaporation [19,20]. The size of liposomes ensures the final size of lipobeads.…”

Section: Polymerization Within Liposomal Nano-/microreactorsmentioning

confidence: 99%

“…In this case, lipid bilayer adsorption on the surface of hydrogel particles prepared separately was promoted via Coulombic attraction between the charged microgels and oppositely charged lipids [7,23,24], hydration of lipid films with micro-or nanogel suspension [2,3], introduction of hydrophobic anchors at the microgel surface around which adsorbing lipids may assemble [6,[25][26][27][28], centrifugation of microgels onto a lipid film [29], microfluidic flowing [21], and emulsification [30][31][32].…”

Section: Strategies Of Lipobeads Preparationmentioning

confidence: 99%

“…If the ultimate goal was to engineer the giant lipobeads greater than 1 μm, the gentle hydration of a phospholipid cake [17,36] or a hybrid agarose/lipid film [unpublished], electroformation [12,22,33,34], emulsification [32], and reverse phase evaporation [1,13,30] were used without any agitation of the lipid formulations.…”

Section: Polymerization Within Liposomal Nano-/microreactorsmentioning

confidence: 99%

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Lipobeads

Казаков¹

2017

Liposomes

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A combination of lipid bilayer and cross-linked polymer network is the logical step in development of polymeric and liposomal nanoscopic systems to provide the natural level of functionality. From liposomal systems, lipobeads borrow the welldeveloped methods for preparation, diversity of lipids to control the properties of a lipid bilayer, biocompatibility of the lipid bilayer, possibility to vary size and morphology (passive targeting), availability of the external surface for attachment of various ligands (active targeting), encapsulation efficiency of both hydrophilic and hydrophobic molecules. Mechanical stability of the lipid bilayer and environmental responsiveness of the whole structure are the properties that hydrogel core brings to the new construct. The reports reviewed in this chapter demonstrate that lipobeads of nanometer and micrometer sizes can be prepared in different media, retain their stimuli responsiveness under physiological conditions, exhibit both reversible and irreversible aggregation, can be loaded with both small and high molecular weight molecules. As a platform for drug delivery systems, lipobeads have already been loaded with chemotherapeutics, malaria vaccine, and dermatological agent providing different controlled release profiles without leakage. Consecutive multistep triggering, new schemes of drug release, combined drug delivery, vesobeads, proteolipobeads, enzyme-containing lipobeads, and hemi-lipobeads are the directions for their future development.

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Section: Liposomes 54mentioning

confidence: 99%

Section: Polymerization Within Liposomal Nano-/microreactorsmentioning

confidence: 99%

Section: Polymerization Within Liposomal Nano-/microreactorsmentioning

confidence: 99%

Section: Strategies Of Lipobeads Preparationmentioning

confidence: 99%

Section: Polymerization Within Liposomal Nano-/microreactorsmentioning

confidence: 99%

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Lipobeads

Казаков¹

2017

Liposomes

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Cytoskeletal‐like Supramolecular Assembly and Nanoparticle‐Based Motors in a Model Protocell

Kumar

Patil

et al. 2011

Angewandte Chemie

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We thank the EPSRC and the China Scholarship Council (X.Y.) for supporting this study, James Grieve (particle tracking, viscosity measurements), Andy Salmon (aspiration experiments), Stuart Bellamy (optical microscopy), and David Williams (data analysis) for help with experiments, and the Bristol Centre for Nanoscience and Quantum Information and Bristol Wolfson Bioimaging Suite for providing facilities.Supporting information (including full details of experimental methods) for this article is available on the WWW under http://dx.

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Cytoskeletal‐like Supramolecular Assembly and Nanoparticle‐Based Motors in a Model Protocell

Kumar

Patil

et al. 2011

Angew Chem Int Ed

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Reconstitution of cellular functions within synthetic constructs such as lipid or surfactant vesicles represents a central objective of protocell research. [1][2][3][4] Realizing this goal has important implications for the design and construction of soft, water-based compartmentalized systems with lifelike properties, and should provide unique opportunities in synthetic biology and bionanotechnology, [5] as well as research on the origins of life.[6] Recent studies have developed synthetic protocell models based on vesicles capable of polymerase chain reaction (PCR) induced DNA amplification, [7] gene expression of single components [8] or cascading networks, [9,10] biochemical transformations, [11] poly(adenylic acid) synthesis, [12] or RNA replication. [13] In contrast, there have been relatively few reports on the reconstitution of dynamically self-assembled cytoskeletal-like structures within protocell models. [14][15][16][17][18] Whilst these studies represent important steps towards the confirmation and refinement of biological mechanisms of cytoskeletal assembly and organization, the possibility of mimicking the cytoskeleton synthetically, that is, using the reversible noncovalent supramolecular assembly of nonbiological components, has not, to the best of our knowledge been explicitly explored. In contrast to previous studies on the encapsulation of polymer gels in vesicles, [20][21][22][23] herein we report the noncovalent assembly of a supramolecular network within the interior of phospholipid vesicles using the in situ enzymatic dephosphorylation of small-molecule, amino-acid-based components. Moreover, we exploit supramolecular gelation within the vesicles to generate robust, soft microcompartments capable of chemically derived self-propulsion arising from the platinum-nanoparticle-catalyzed decomposition of hydrogen peroxide (H 2 O 2 ). [24][25][26][27][28][29][30] Aqueous suspensions of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) vesicles comprising supramolecular hydrogel interiors were prepared using an inverted emulsion method [31,32] combined with in situ alkaline phosphatase-mediated dephosphorylation of N-fluorenylmethylcarbonyl-tyrosine-(O)-phosphate (Fmoc-TyrP; see Scheme 1 in the Supporting Information).[33] As described previously, [33,34] the latter process results in the noncovalent selfassembly of Fmoc-Tyr molecules into bundles of supramolecular nanofilaments that exhibit solid-like viscoelastic properties (see Figure S1 in the Supporting Information), and can be reversibly disassembled by heating the hydrogels above the gel-sol transition temperature (typically around 45 8C). Optical microscopy studies indicated that the amino acid/enzymecontaining hydrogelled vesicles were not aggregated, spherical in shape, 1-25 mm in diameter, and structurally stable when dispersed in water for several months at room temperature, or freeze-dried and investigated by SEM (Figure 1 a,b). The hydrogelled vesicles could be sedimented and rehydrated by slow centrifugation at 120 g for 30 min...

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Preparation of aqueous gel beads coated by lipid bilayers

Abstract: Novel giant liposome microcapsules have been fabricated based on aqueous gel cores encapsulated with a lipid bilayer. The method involves templating of lipid-stabilised water-in-oil emulsions after gelling the aqueous phase with a suitable hydrocolloid.

Cited by 17 publications

References 12 publications

Lipobeads

Lipobeads

Cytoskeletal‐like Supramolecular Assembly and Nanoparticle‐Based Motors in a Model Protocell

Cytoskeletal‐like Supramolecular Assembly and Nanoparticle‐Based Motors in a Model Protocell

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