Preparation of Au nanoparticles-coated polystyrene beads and its application in protein immobilization

Cao, Yuan‐Cheng; Wang, Zhan; Jin, Xin; Hua, Xiao-Feng; Liu, Manxi; Zhao, Yuan‐Di

doi:10.1016/j.colsurfa.2008.10.002

Cited by 26 publications

(15 citation statements)

References 38 publications

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“…The PS beads (2 lm in diameter) modified by carboxyl groups on the surface were used and purchased from baseline (Tianjin, China) because their density (q ¼ 1.05 gÁcm À3 ) is very close to that of bacterial cells (q ¼ 1.05 gÁcm À3 to 1.07 gÁcm À3 ) and their biocompatibility is very good. 45 Recombinant protein A (Protein A, Bioss, Inc., USA) was dissolved to a concentration of 5 g/l in PBS (pH 7.4). 2-(N-morpholino)ethanesulfonic acid (MES), N-hydroxysuccinimide (NHS, MW 191.7) and N-(3-dimethylaminopropyl)-N 0 -ethylcarbodiimide (EDC, MW 217.13) were purchased from Sigma (USA) and dissolved in MES at 10 mg/ml.…”

Section: Reagentsmentioning

confidence: 99%

Construction and operation of a microrobot based on magnetotactic bacteria in a microfluidic chip

Chen

Wei

et al. 2012

Biomicrofluidics

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Magnetotactic bacteria (MTB) are capable of swimming along magnetic field lines. This unique feature renders them suitable in the development of magnetic-guided, auto-propelled microrobots to serve in target molecule separation and detection, drug delivery, or target cell screening in a microfluidic chip. The biotechnology to couple these bacteria with functional loads to form microrobots is the critical point in its application. Although an immunoreaction approach to attach functional loads to intact MTB was suggested, details on its realization were hardly mentioned. In the current paper, MTB-microrobots were constructed by attaching 2 μm diameter microbeads to marine magnetotactic ovoid MO-1 cells through immunoreactions. These microrobots were controlled using a special control and tracking system. Experimental results prove that the attachment efficiency can be improved to ∼30% via an immunoreaction. The motility of the bacteria attached with different number of loads was also assessed. The results show that MTB can transport one load at a velocity of ∼21 μm/s and still move and survive for over 30 min. The control and tracking system is fully capable of directing and monitoring the movement of the MTB-microrobots. The rotating magnetic fields can stop the microrobots by trapping them as they swim within a circular field with a controllable size. The system has potential use in chemical analyses and medical diagnoses using biochips as well as in nano/microscale transport.

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Section: Reagentsmentioning

confidence: 99%

Construction and operation of a microrobot based on magnetotactic bacteria in a microfluidic chip

Chen

Wei

et al. 2012

Biomicrofluidics

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“…The typical ways to prepare core-shell nanocomposites are self-assembly, in situ reduction, and seed-grow approaches [9,[22][23][24][25][26]. The traditional self-assembly synthesis routes are based on a two-step process, synthesis of Au colloids, and immobilization on supports via electrostatic effect, covalent interaction, or ligand coordination [26][27][28]. In these methods, a necessary step is to modify the cores with the surface functional groups, such as -NH 2 , -SH, positive charges and polyelectrolytes [29][30][31][32][33], which are effective for the connection of small Au NPs.…”

Section: Introductionmentioning

confidence: 99%

Controllable synthesis and catalysis application of hierarchical PS/Au core–shell nanocomposites

Zhou

Ren

et al. 2012

Journal of Colloid and Interface Science

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“…PS microsphere was combined with metallic NPs to prepare SERS substrates due to the strong adsorption capacity, strong oxygen permeability, and the possibility of surface functionalization. Several methods have been developed to fabricate metal/PS composites, for example, self-assembly procedures, surface reduction reactions, and magnetron sputtering ( Wei et al, 2007 ; Ishida et al, 2008 ; Cao et al, 2009 ; Chang et al, 2010 ; Kim et al, 2010 ; Jian et al, 2013 ; Balčytis et al, 2017 ). Ocwieja et al (2018) produced AuNP-PS nanoparticles/particles by forming a layer of positively charged gold nanoparticles on polystyrene.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Plasmonic Ag@PS Composite via Seed-Mediated In Situ Growth Method and Application in SERS

Tian

Kong

et al. 2022

Front. Chem.

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The colloidal polystyrene (PS) was synthesized and decorated with silver nanoparticles (Ag NPs). The plasmonic Ag@PS nanocomposite was prepared by loading Ag NPs on PS microsphere through a seed-mediated in situ growth route. The property of Ag NPs deposited on the PS microsphere could be precisely controlled by adjusting the concentration of the chemicals used in the growth medium. The growth step is only limited by the diffusion of growing species in the growth media to the surface of the Ag seed. The Ag@PS prepared via the in situ growth method exhibited two advantages compared with the self-assembled PS/Ag. First, the high-density of Ag NPs were successfully deposited on the surface of PS as the electroless-deposited Ag seed process, which brings nearly three times SERS enhancement. Second, the rapid preparation process for in situ growth method (half an hour, 10 h for the self-assembled method). The PS/Ag could detect Nile blue A (NBA) down to 10–7 M by SERS. Furthermore, the plasmonic Ag@PS SERS substrate was used for pesticide identification. The on-site monitoring malachite green (MG) from fish was achieved by portable Raman spectrometer, and the limit of detection (LOD) was 0.02 ppm. The Ag@PS substrate has also shown capability for simultaneously sensing multiple pesticides by SERS.

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Preparation of Au nanoparticles-coated polystyrene beads and its application in protein immobilization

Cited by 26 publications

References 38 publications

Construction and operation of a microrobot based on magnetotactic bacteria in a microfluidic chip

Construction and operation of a microrobot based on magnetotactic bacteria in a microfluidic chip

Controllable synthesis and catalysis application of hierarchical PS/Au core–shell nanocomposites

Preparation of Plasmonic Ag@PS Composite via Seed-Mediated In Situ Growth Method and Application in SERS

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