Preparation of Bilayer Molecular Assembly from Fatty Acid and Detergent

Taguchi, Shogo; Kimura, Yuta; Tachibana, Yasuaki; Yamamoto, Takuji; Umakoshi, Hiroshi

doi:10.1252/kakoronbunshu.47.51

Cited by 2 publications

(2 citation statements)

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“…Here, an OA vesicle was used as a source of a bilayer membrane [14]. We also discovered that the size of the OA vesicle decreased as the OA to CHAPSO mixing molar ratio (X OA = [OA]/([OA] + [CHAPSO])) decreased [13]. The size reduction demonstrated that the CHAPSO molecule acted as a detergent fluidizing the OA bilayer membrane.…”

Section: Introductionmentioning

confidence: 93%

“…The fatty-acid-rich bicelle had a bilayer membrane structure derived from fatty acids [12] and could be used as the parent. We have previously used a fluorescent probe, 6-dodecanoyl-N,N-dimethyl-2-naphthylamine (Laurdan), to analyze the phase state of the fatty-acid-rich assembly [13]. The ordered and the disordered phase were found to coexist in the bilayer of oleic acid (OA), as a fatty acid, in a solution of 3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxypropane sulfonate (CHAPSO), as a detergent.…”

Section: Introductionmentioning

confidence: 99%

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The Fluidization Effect of a Bilayer Membrane on a Fatty Acid Vesicle by a Detergent

et al. 2021

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A bicelle, which is a bilayer molecular assembly, can be prepared by fluidizing a vesicle in the presence of a detergent. We investigated the effect of two different detergents, 3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxypropane sulfonate (CHAPSO) and Triton X-100 (TX), on the formation of a bicelle from a vesicle containing oleic acid (OA) and the detergent molecules. The fluidization effect of the detergent was evaluated using the membrane packing density, which we measured using the fluorescent probe method with Laurdan, in conjunction with transmission electron microscopy to examine the morphology of the prepared bilayer molecular assemblies. As a result, it was discovered that the OA/CHAPSO system could form a heterogeneous phase with the highest packing density, implying that CHAPSO was the better detergent for a bicelle preparation, whereas the OA/TX system formed a disordered phase with the lowest packing density.

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Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%