Preparation of bioactive β-tricalcium phosphate microspheres as bone graft substitute materials

Li, Bo; Liu, Zhongning; Yang, Jingwen; Yi, Zhongchao; Xiao, Wenqian; Li, Xue; Yang, Xiaoling; Xu, Wei; Liao, Xiaoling

doi:10.1016/j.msec.2016.03.040

Cited by 31 publications

(19 citation statements)

References 34 publications

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“…However, poor degradability hinders its clinical application (Bohner et al, 2005). In addition, improvement in maturity of new bone formed by β-TCP is needed as our previous study demonstrated (Li, Liu, et al, 2017). However, it remains a challenge to maintain the needed mechanical support during the initial stage of implantation and to avoid detrimental effects on surrounding newly formed bone tissues due to rapid degradation at longer times (Yuan et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…However, it remains a challenge to maintain the needed mechanical support during the initial stage of implantation and to avoid detrimental effects on surrounding newly formed bone tissues due to rapid degradation at longer times (Yuan et al, 2001). In addition, improvement in maturity of new bone formed by β-TCP is needed as our previous study demonstrated (Li, Liu, et al, 2017). Therefore, BCP ceramic, a composite mixture of HA and β-TCP, has been developed to overcome the weaknesses of pure HA and β-TCP.…”

Section: Discussionmentioning

confidence: 99%

“…As shown in Figure 1a, the S/O/W emulsification method was adopted to prepare Sr-BCP microspheres, according to our previous report with modifications (Li, Liu, et al, 2017). The Sr-HA powder (10 wt%) was added to the oil phase comprising PVB (5 wt%) and DCM.…”

Section: Preparation Of Sr-bcp Microspheresmentioning

confidence: 99%

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Strontium‐substituted biphasic calcium phosphate microspheres promoted degradation performance and enhanced bone regeneration

Chen

Liu

Jiang

et al. 2019

J Biomedical Materials Res

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Biphasic calcium phosphate (BCP) ceramics are the subject of much attention for use as bone replacement material. However, it remains a challenge to promote the degradation and osteoinductivity performances of BCP ceramics. In this work, novel BCP ceramic microspheres with good degradation and excellent osteoinductivity were prepared through high-content strontium (Sr) doping. The in vitro results indicated that the Sr10-BCP, Sr40-BCP, and Sr80-BCP microspheres all had their HA crystals partially transformed to the beta tricalcium phosphate phase following high-temperature sintering because of Ca-deficient HA formed by the partial substitution of Ca ions by Sr ions. In addition, the degradation rate was increased with the doping of increasing amounts of Sr. All prepared microspheres enhanced human bone marrow-derived mesenchymal stem cells attachment and proliferation. Specifically, among these modified microspheres, the Sr40-BCP microspheres showed the highest osteogenic potential. Furthermore, Sr40-BCP and HA microspheres were implanted in a calvarial defect model of rat to evaluate the in vivo bone augmentation ability. The results indicated that Sr40-BCP microspheres degraded more completely and significantly promoted new bone regeneration compared with HA microspheres. In conclusion, Sr40-BCP microspheres have excellent potential for degradation and bone regeneration and are promising osteogenic materials. K E Y W O R D Sbiphasic calcium phosphate, bone regeneration, degradation, microspheres, strontiumsubstituted

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Strontium‐substituted biphasic calcium phosphate microspheres promoted degradation performance and enhanced bone regeneration

Chen

Liu

Jiang

et al. 2019

J Biomedical Materials Res

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“…The β-TCP scaffolds have the advantages of biocompatibility and biodegradability, and favorable osteogenesis and angiogenesis properties (31)(32)(33). Several studies have demonstrated that using β-TCP scaffolds combined with BMSCs could promote bone regeneration in vitro and in vivo (18,(34)(35)(36). The porous structure of β-TCP provides a scaffold for the adhesion and growth of BMSCs while the connection between pores facilitates the blood supply of the scaffolds.…”

Section: Introductionmentioning

confidence: 99%

A specific affinity cyclic peptide enhances the adhesion, expansion and proliferation of rat bone mesenchymal stem cells on β‑tricalcium phosphate scaffolds

et al. 2019

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osteonecrosis of the femoral head (onFH) is a common osteological disease. Treatment of onFH prior to the collapse of the femoral head is critical for increasing therapeutic efficiency. Tissue engineering therapy using bone mesenchymal stem cells (BMSCs) combined with a scaffold is a promising strategy. However, it is currently unclear how to improve the efficiency of BMSC recruitment under such conditions. In the present study, a specific cyclic peptide for Sprague-Dawley rat BMSCs, CTTNPFSLC (known as C7), was used, which was identified via phage display technology. Its high affinity for BMSCs was demonstrated using flow cytometry and fluorescence staining. Subsequently, the cyclic peptide was placed on β-tricalcium phosphate (β-TcP) scaffolds using absorption and freeze-drying processes. Adhesion, expansion and proliferation of BMSCs was investigated in vitro on the c7-treated β-TcP scaffolds and compared with pure β-TCP scaffolds. The results revealed that C7 had a promoting effect on the adhesion, expansion and proliferation of BMSCs on β-TCP scaffolds. Therefore, C7 may be effective in future tissue engineering therapy for ONFH.

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“…TCP microspheres present a higher rate of in vivo resorption when compared to dense blocks [9,10] due to their larger specific surface area, porosity and surface reactivity, increased rate of bone ingrowth, minimized inflammatory responses, higher packing, and good flowability. Therefore, they are suitable for many applications [11][12][13] such as filling bone defects [10,14,15], formulating injectable pastes and cements [16][17][18][19], drugs, genes and cells carriers [20][21][22], being used for thermal-spray coatings [23], and serving as raw materials for 3D printed and robocasted bone implants [24,25]. The shape and size of implantable TCP microspheres should be carefully controlled since it has been shown that they determine the extent of tissue reaction [26] as well as the handling properties of cement pastes and putties (mainly packing and injectability) [19,27].…”

Section: Introductionmentioning

confidence: 99%

Preparation and properties of α-tricalcium phosphate microspheres by spray drying

Renó

Carrodeguas²,

Motisuke

et al. 2019

Cerâmica

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a-TCP microspheres were obtained by atomizing aqueous slurries containing 50 wt% of low crystallinity α-TCP and 1 wt% of citric acid. Poly(ethylene glycol) and poly(vinyl alcohol) were tested as a binder. The spray drying process yielded to microspheres with mean diameter around 20 μm and sphericity (horizontal and vertical length ratio) closest to one, which is essential to many applications, such as drug delivery and injectability of cements. The addition of binders was necessary to decrease the amount of loose particles and to improve powder flowability but it also caused a reduction in wettability and surface area. Best results were achieved with 3 wt% of PVA, as the microspheres presented the highest specific surface area, wettability, and sphericity. The microspheres prepared in this study may be used as injectable bone cements precursor since their geometry and flowability would favor paste homogeneity and injectability overcoming many drawbacks of today’s injectable bone cements.

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Preparation of bioactive β-tricalcium phosphate microspheres as bone graft substitute materials

Cited by 31 publications

References 34 publications

Strontium‐substituted biphasic calcium phosphate microspheres promoted degradation performance and enhanced bone regeneration

Strontium‐substituted biphasic calcium phosphate microspheres promoted degradation performance and enhanced bone regeneration

A specific affinity cyclic peptide enhances the adhesion, expansion and proliferation of rat bone mesenchymal stem cells on β‑tricalcium phosphate scaffolds

Preparation and properties of α-tricalcium phosphate microspheres by spray drying

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