Preparation of biocompatible magnetic microspheres with chitosan

Nasra, Mohamed K.; Mohamed, Moustafa M.; Elblbesy, Mohamed A.; Hefney, Bothaina A.

doi:10.4236/jbnb.2011.22024

Cited by 10 publications

(4 citation statements)

References 26 publications

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“…The results of FT-IR spectra strongly match those mentioned by Jiang et al [41] who indicated the presence of stretching vibrations of hydroxyl peak at 3427 cm −1 and the occurrence of strong peaks at 2925 and 2855 cm −1 confirmed the existence C-H stretching vibration of the polymer backbone. Similarly, Nasra et al [42] reported that the stretch vibrations of C-O are located at 1084 and 1032 cm −1 . For cross-linked chitosan microspheres, the existing of an additional peak at 1656 cm −1 , which clarifies stretching vibrations of the C=N bond, emphasizes the formation of Schiff's base as a result of the reaction.…”

Section: Characterization Of Chitosan Beadsmentioning

confidence: 84%

Lipase from Rhizopus oryzae R1: in-depth characterization, immobilization, and evaluation in biodiesel production

Helal

Abdelhady

Abou-Taleb

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Rhizopus species is among the most well-known lipase producers, and its enzyme is suitable for use in many industrial applications. Our research focuses on the production of lipase utilizing waste besides evaluating its applications. Results An extracellular lipase was partially purified from the culture broth of Rhizopus oryzae R1 isolate to apparent homogeneity using ammonium sulfate precipitation followed by desalting via dialysis. The partially purified enzyme was non-specific lipase and the utmost activity was recorded at pH 6, 40 °C with high stability for 30 min. The constants Km and Vmax, calculated from the Lineweaver-Burk plot, are 0.3 mg/mL and 208.3 U/mL, respectively. Monovalent metal ions such as Na+ (1 and 5 mM) and K+ (5 mM) were promoters of the lipase to enhance its activity with 110, 105.5, and 106.5%, respectively. Chitosan was used as a perfect support for immobilization via both adsorption and cross-linking in which the latter method attained immobilization efficiency of 99.1% and reusability of 12 cycles. The partially purified enzyme proved its ability in forming methyl oleate (biodiesel) through the esterification of oleic acid and transesterification of olive oil. Conclusion The partially purified and immobilized lipase from Rhizopus oryzae R1 approved excellent efficiency, reusability, and a remarkable role in detergents and biodiesel production.

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Section: Characterization Of Chitosan Beadsmentioning

confidence: 84%

Lipase from Rhizopus oryzae R1: in-depth characterization, immobilization, and evaluation in biodiesel production

Helal

Abdelhady

Abou-Taleb

et al. 2021

Journal of Genetic Engineering and Biotechnology

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“…CH stretch vibrations were at 2921, 2871 cm −1 , COC—at 1071 cm −1 , 1649 cm −1 (amide I), 1554, 1599 cm −1 (amide II), and 1377 cm −1 (CH 3 ) (CH 3 CONH‐groups of chitosan). IR spectrum of CS‐coated magnetite particles was described earlier . In the spectrum of Fe 3 O 4 /CS/EM [Figure (A), 2 ] and IPEC CS/EM [Figure (B), 1 ] the absorption bands in the region of 1632–1708 cm −1 , ν COO − = 1555, 1400 cm −1 , and ν CO = 1074 cm −1 belonging to CS–MAC interpolyelectrolyte complex were presented earlier .…”

Section: Resultsmentioning

confidence: 70%

Interpolyelectrolyte complexes of maleic acid copolymers and chitosan for stabilization and functionalization of magnetite nano‐ and microparticles

Самойлова

Тихонов

Krayukhina

et al. 2013

J of Applied Polymer Sci

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A facile method of preparation of stabilized and functionalized nano-and microparticles of magnetite by successive application of oppositely charged polymers with a regular structure of macromolecular chains (chitosan and maleic acid copolymers) onto Fe 3 O 4 core are developed. This approach makes it possible to create two types of magnetite interpolyelectrolyte shells, containing carboxylic or amino groups in outer layer of shell. Composition and magnetic properties of composite particles depend on nature of the copolymer of maleic acid, reaction conditions and size of obtained particles. The carboxylic groups of copolymer in outer layer of interpolyelectrolyte shell were converted into reactive anhydride groups by heating. Thermal treatment also leads to covalent crosslinking of shell and improves stability of composites both in acidic and alkaline media. Horseradish peroxidase was successfully immobilized onto covalently crosslinked and activated microparticles of Fe 3 O 4 in aqueous medium without of condensing agents. The proposed reproducible and low-cost technique does not use toxic reagents or solvents at all stages, including preparation of Fe 3 O 4 , formation, activation and crosslinking, of magnetite shell, modification of activated surface of composite particles.

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“…The dissolved chitosan was added dropwise on the magnetic fluid. Formed chitosan magnetic microspheres were washed with deionized water and soaked in 1, 3, and 5 mol % glutaraldehyde solution for 2 hrs and then washed with deionized water [17].…”

Section: Cross-linking Methodsmentioning

confidence: 99%

Magnetic Microsphere an Emerging Drug Delivery System

Sharma¹,

Sharma²

2017

Asian J Pharm Clin Res

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The drug delivery system has been advanced to release the drug according to the body requirement during the entire period of treatment and also for the delivery at the targeted site. Several novel drug delivery systems have emerged encompassing different route of administration to achieve controlled and targeted drug delivery, magnetic microsphere carrier being one of them. Magnetic microsphere is an alternative to traditional radiation methods. As the traditional radiation methods use highly penetrating radiation that is absorbed throughout the body and cause side effects hence its use is limited. Therefore, a safe and effective alternate is needed like magnetic microsphere. The excessive circulating drug particles are minimized by this delivery system. Moreover, the aim of specific targeting is to enhance the effectiveness of drug delivery and at the same time to lessen the toxicity and side effects. Magnetic carriers receive magnetic responses to a magnetic field from incorporated materials that are used for magnetic microsphere are chitosan, dextran, etc. One of the most utilized magnetic microspheres is serum albumine whether from human or other suitable animals. Drug release from the albumin microsphere can be controlled by various stabilization procedures. Overall, the targeted magnetic microsphere is much valuable novel drug delivery system for what more work have to be done. By knowing the importance of all this, the present paper reviews the mechanism, preparation, and applications of magnetic microspheres. As the targeted drug delivery system implies selective and effective localization of drug into the target at therapeutic concentrations with limited access to non-target sites. Magnetic microspheres hold great promises for reaching the goal of controlled and site-specific drug delivery.

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Preparation of biocompatible magnetic microspheres with chitosan

Abstract: Microsphere is a term used for small spherical particles, with diameters in the micrometer range (typically 1 μm to 1000 μm (1 mm)

Cited by 10 publications

References 26 publications

Lipase from Rhizopus oryzae R1: in-depth characterization, immobilization, and evaluation in biodiesel production

Lipase from Rhizopus oryzae R1: in-depth characterization, immobilization, and evaluation in biodiesel production

Interpolyelectrolyte complexes of maleic acid copolymers and chitosan for stabilization and functionalization of magnetite nano‐ and microparticles

Magnetic Microsphere an Emerging Drug Delivery System

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