Preparation of BMP-2 loaded MPEG-PCL microspheres and evaluation of their bone repair properties

Kong, Deyin; Shi, Yanchao; Gao, Yan; Fu, Mengguang; Kong, Shengli; Lin, Guimei

doi:10.1016/j.biopha.2020.110516

Cited by 25 publications

(19 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To address this problem, BMP‐2‐immobilized PEEK was assessed and the positive effects for the osteogenic differentiation of rBMSCs were demonstrated. However, this direct loading method discounted the activity of BMP‐2 and it was difficult to achieve lasting effects 44–46 . To achieve the stable and sustained release of BMP‐2 in the implants, we principally employed PLGA microspheres loaded with pBMP‐2 to realize its continuous release，allowing it to sustainedly transfect cells for the transcription of BMP‐2 over time.…”

Section: Discussionmentioning

confidence: 99%

Construction of a BMP‐2 gene delivery system for polyetheretherketone bone implant material and its effect on bone formation in vitro

Qin

Gan

et al. 2022

J Biomed Mater Res

View full text Add to dashboard Cite

Polyetheretherketone (PEEK) has been widely investigated for improving its biological inert to enable it to achieve stronger osteogenic capability and to be a promising material in implant fields. The most important mechanism that makes a successful implantation is osteointegration. Surface modification is an appropriate method to maintain the excellent mechanical properties of PEEK and simultaneously endow PEEK certain biological characters. In this work, we attempted to shape the nanotopography of PEEK surface by nitrogen low-temperature plasma and polydopamine coating on the surface as a secondary reaction platform to bond the aminated poly (lactic-co-glycolic acid) (PLGA) microspheres encapsulating the BMP-2 gene for enhancing the biological activity. Scanning electron microscope, atomic force microscopy, X-ray photoelectron spectroscopy and water contact angle (CA) measurements were applied to characterize the surface of modified or untreated PEEK. Surface characterization showed that the modification was successfully performed on PEEK including a rougher and more hydrophilic surface with nanotopographic features. The influence on cell adhesion, proliferation and differentiation was evaluated by culturing of rat bone marrow mesenchymal stem cells on different modified PEEK substrates in vitro. The biological results indicated that the low-temperature plasma treatment and PDA-coating on PEEK significantly promoted cell adhesion and proliferation. And the osteogenic differentiation was effectively improved by BMP-2 gene releasing from PLGA-NH 2 microspheres. The results showed that this novel biological surface modification endowed PEEK with outstanding bioactivity and osteogenic ability, providing a theoretical basis for application in the field of implantation. K E Y W O R D S biomaterial, improved cell adhesion, PLGA-NH 2 microspheres, promoted osteogenic differentiation 1 | INTRODUCTION For repairing bone defects resulting from surgery, trauma, diseases, and congenital malformations, titanium-based alloys have become the Shuang Qin and Zhengkuan Lu contributed equally to this work

show abstract

Section: Discussionmentioning

confidence: 99%

Construction of a BMP‐2 gene delivery system for polyetheretherketone bone implant material and its effect on bone formation in vitro

Qin

Gan

et al. 2022

J Biomed Mater Res

View full text Add to dashboard Cite

show abstract

“…Strong local retention of rhBMP-2 would permit significant reduction of its therapeutic dosing. Such retention was implemented, for example, by covalent linkage of rhBMP-2 to a carrier material, such as to fibrin hydrogels (22), by noncovalent yet specific interactions to a carrier material, such as to heparan sulfate-supplemented collagen matrices (23) or to heparin microparticles (24), or by biophysical-or biochemical-based retention, such as through rhBMP-2 encapsulation into polymer microspheres (25). Alternative strategies also explored the in vivo delivery of rhBMP-2-expressing mesenchymal stem cells to achieve sustained release of low doses of rhBMP-2 (15,26).…”

Section: Discussionmentioning

confidence: 99%

Engineered bridge protein with dual affinity for bone morphogenetic protein-2 and collagen enhances bone regeneration for spinal fusion

et al. 2021

View full text Add to dashboard Cite

The revolutionizing efficacy of recombinant human bone morphogenetic protein (rhBMP-2) for clinical spinal fusion is hindered by safety issues associated with the high dose required. However, it continues to be widely used, for example, in InFUSE Bone Graft (Medtronic). Here, we developed a translational protein engineering–based approach to reduce the dose and thereby improve the safety of rhBMP-2 delivered in a collagen sponge, as in InFUSE Bone Graft. We engineered a bridge protein with high affinity for rhBMP-2 and collagen that can be simply added to the product’s formulation, demonstrating improved efficacy at low dose of rhBMP-2 in two mouse models of bone regeneration, including a newly developed spinal fusion model. Moreover, the bridge protein can control the retention of rhBMP-2 from endogenous collagenous extracellular matrix of tissue. Our approach may be generalizable to other growth factors and collagen-based materials, for use in many other applications in regenerative medicine.

show abstract

“…At present, microspheres are mainly divided into natural polymer microspheres and synthetic polymer microspheres. The microspheres are wrapped with BMP through microcapsules, processed into composite scaffolds, and implanted into bone defects to achieve the purpose of continuous induction of bone regeneration ( Bai et al, 2020 ; Injamuri et al, 2020 ; Kong et al, 2020 ; Koons et al, 2021 ; Rajabnejadkeleshteri et al, 2021 ).…”

Section: Applications Of Bmps In Bone Tissue Engineeringmentioning

confidence: 99%

Application of BMP in Bone Tissue Engineering

Zhu

Liu

Wang

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

At present, bone nonunion and delayed union are still difficult problems in orthopaedics. Since the discovery of bone morphogenetic protein (BMP), it has been widely used in various studies due to its powerful role in promoting osteogenesis and chondrogenesis. Current results show that BMPs can promote healing of bone defects and reduce the occurrence of complications. However, the mechanism of BMP in vivo still needs to be explored, and application of BMP alone to a bone defect site cannot achieve good therapeutic effects. It is particularly important to modify implants to carry BMP to achieve slow and sustained release effects by taking advantage of the nature of the implant. This review aims to explain the mechanism of BMP action in vivo, its biological function, and how BMP can be applied to orthopaedic implants to effectively stimulate bone healing in the long term. Notably, implantation of a system that allows sustained release of BMP can provide an effective method to treat bone nonunion and delayed bone healing in the clinic.

show abstract

Preparation of BMP-2 loaded MPEG-PCL microspheres and evaluation of their bone repair properties

Cited by 25 publications

References 42 publications

Construction of a BMP‐2 gene delivery system for polyetheretherketone bone implant material and its effect on bone formation in vitro

Construction of a BMP‐2 gene delivery system for polyetheretherketone bone implant material and its effect on bone formation in vitro

Engineered bridge protein with dual affinity for bone morphogenetic protein-2 and collagen enhances bone regeneration for spinal fusion

Application of BMP in Bone Tissue Engineering

Contact Info

Product

Resources

About