Preparation of bromomethylated poly(2,6-dimethyl-1,4-phenylene oxide) hollow fiber cation-exchange membranes and immobilization of cellulase thereon

Cheng, Zhenfeng; Wu, Cuiming; Yang, Wei; Xu, Tongwen

doi:10.1016/j.memsci.2010.04.036

Cited by 18 publications

(8 citation statements)

References 32 publications

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“…Another study conducted on immobilised cellulase demonstrated that the enzyme preparation was quite stable in a wide pH range (pH 1.5 to 12.0) but the activity of natural/free cellulase was found to be reduced for pH 6.0 [ 27 ]. The studies conducted on cellulase immobilisation on cation-exchange membranes demonstrated a broader pH range of 3.8 similar to the present work [ 28 ].…”

Section: Resultssupporting

confidence: 85%

Suitability of magnetic nanoparticle immobilised cellulases in enhancing enzymatic saccharification of pretreated hemp biomass

Abraham

Verma

Barrow

et al. 2014

Biotechnol Biofuels

233

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BackgroundPrevious research focused on pretreatment of biomass, production of fermentable sugars and their consumption to produce ethanol. The main goal of the work was to economise the production process cost of fermentable sugars. Therefore, the objective of the present work was to investigate enzyme hydrolysis of microcrystalline cellulose and hemp hurds (natural cellulosic substrate) using free and immobilised enzymes. Cellulase from Trichoderma reesei was immobilised on an activated magnetic support by covalent binding and its activity was compared with that of the free enzyme to hydrolyse microcrystalline cellulose and hemp hurds on the basis of thermostability and reusability.ResultsUp to 94% protein binding was achieved during immobilisation of cellulase on nanoparticles. Successful binding was confirmed using Fourier transform infrared spectroscopy (FTIR). The free and immobilised enzymes exhibited identical pH optima (pH 4.0) and differing temperature optima at 50°C and 60°C, respectively. The K M values obtained for the free and immobilised enzymes were 0.87 mg/mL and 2.6 mg/mL respectively. The immobilised enzyme retained 50% enzyme activity up to five cycles, with thermostability at 80°C superior to that of the free enzyme. Optimum hydrolysis of carboxymethyl cellulose (CMC) with free and immobilised enzymes was 88% and 81%, respectively. With pretreated hemp hurd biomass (HHB), the free and immobilised enzymes resulted in maximum hydrolysis in 48 h of 89% and 93%, respectively.ConclusionThe current work demonstrated the advantages delivered by immobilised enzymes by minimising the consumption of cellulase during substrate hydrolysis and making the production process of fermentable sugars economical and feasible. The activity of cellulase improved as a result of the immobilisation, which provided a better stability at higher temperatures. The immobilised enzyme provided an advantage over the free enzyme through the reusability and longer storage stability properties that were gained as a result of the immobilisation.

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Section: Resultssupporting

confidence: 85%

Suitability of magnetic nanoparticle immobilised cellulases in enhancing enzymatic saccharification of pretreated hemp biomass

Abraham

Verma

Barrow

et al. 2014

Biotechnol Biofuels

233

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“…The cation-exchange capacity (CEC) was determined by a titration method (Cheng et al 2010) using sodium hydroxide solution (0.1 mol/L); 0.5 g of SDF from carrot residues was accurately weighed and placed in a 150 ml of conical flask, and then mixed with 100 ml of NaCl solution with a concentration of 5 % and magnetically stirred for 5 min. Sodium hydroxide solution (0.5 ml, 0.1 mol/L) was used to titrate each time and used pH meter to monitor the pH value.…”

Section: Determination Of the Cation-exchange Capacitymentioning

confidence: 99%

Physicochemical and Bioactive Properties of Soluble Dietary Fibers from Blasting Extrusion Processing (BEP)-Extruded Carrot Residues

Gao

Yan

et al. 2015

Food Bioprocess Technol

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A 50 % increase in the content of water-soluble dietary fibers from carrot residues (CRSDF) was obtained by a novel blasting extrusion processing (BEP) with improved water holding capacity and oil retention capacity. A neutral polysaccharide (CRSDF-2) was successfully separated by a DEAE-52-cellulose column chromatography system. The results of high-performance liquid chromatography (HPLC) and gas chromatography (GC) showed that CRSDF-2 contained four sorts of monosaccharides: arabinose, xylose, glucose, and galactose with a molar ratio of 0.58:1.50:1.0:2.02. The average molecular weight of CRSDF-2 was estimated to be approximately 5.98 × 10 4 Da. Moreover, it was illustrated that CRSDF displayed a high cation-exchange capacity and a high adsorption capacity for Pb cation. Furthermore, CRSDF was capable of binding sodium deoxycholate sodium cholate, sodium deoxycholate, and sodium taurocholate. CRSDF could be potentially used as a promising ingredient in functional food and pharmaceutical industries.

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“…Nevertheless, the application of these methods has suffered from the difficulty in recovering nanoparticles from reaction medium, especially when the reaction mixture contains unhydrolyzed solid residues. , Even though the magnetic nanoparticles can be uniquely collected and recycled through a magnetic field, a loss of >10% catalyst was still observed after one separation step from a thick broth with high solid concentrations . Comparatively, polymer materials such as film or fabrics can be facilely separated from reaction media with minimal substrates loss, which is more suitable for continuous operations in mass production. , Many polymeric substrates such as acrylonitrile copolymer membrane, chitosan sponge, electrospun nanofibrous membranes, − hollow fiber cation-exchange membranes, and polycarbonate track-etched membrane, have been used for immobilization of cellulase. However, the bioconversion efficiency of cellulase attached on bulk substrates are often low in view of the limited accessibility of the enzyme to the insoluble biomass. , …”

Section: Introductionmentioning

confidence: 99%

“…30 Comparatively, polymer materials such as film or fabrics can be facilely separated from reaction media with minimal substrates loss, which is more suitable for continuous operations in mass production. 31,32 Many polymeric substrates such as acrylonitrile copolymer membrane, 33 chitosan sponge, 34 electrospun nanofibrous membranes, 35−38 hollow fiber cation-exchange membranes, 39 and polycarbonate track-etched membrane, 40 have been used for immobilization of cellulase. However, the bioconversion efficiency of cellulase attached on bulk substrates are often low in view of the limited accessibility of the enzyme to the insoluble biomass.…”

Section: Introductionmentioning

confidence: 99%

Layered Co-Immobilization of β-Glucosidase and Cellulase on Polymer Film by Visible-Light-Induced Graft Polymerization

Wang

Yuan

Chen

et al. 2019

ACS Appl. Mater. Interfaces

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Exploring a suitable immobilization strategy to improve catalytic efficiency and reusability of cellulase is of great importance to lowering the cost and promoting the industrialization of cellulose-derived bioethanol. In this work, a layered structure with a thin PEG hydrogel as the inner layer and sodium polyacrylate (PAANa) brush as the outer layer was fabricated on low density polyethylene (LDPE) film by visible-light-induced graft polymerization. Two enzymes, β-glucosidase (BG) and cellulase, were separately coimmobilized onto this hierarchical film. As supplementary to cellulase for improving catalytic efficiency, BG was in situ entrapped into the inner PEG hydrogel layer during the graft polymerization from the LDPE surface. After graft polymerization of sodium acrylate on the PEG hydrogel layer was reinitiated, cellulase was covalently attached on the outer PAANa brush layer. Owing to the mild reaction condition (visible-light irradiation and room temperature), the immobilized BG could retain a high activity after the graft polymerization. The immobilization did not alter the optimal pH and temperature of BG or the optimal temperature of cellulase. However, the optimal pH of cellulase shifts to 5.0 after immobilization. Compared with the original activity of single cellulase system and isolated BG/cellulase immobilization system, the dual-enzyme system exhibited 82% and 20% increase in catalytic activity, respectively. The dual-enzyme system could maintain 93% of carboxymethylcellulose sodium salt (CMC) activity after repeating 10 cycles of hydrolysis and 89% of filter paper activity after 6 cycles relative to original activity, exhibiting excellent reusability. This layer coimmobilization system of BG and cellulase on the polymer film displays tremendous potential for practical application in a biorefinery.

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Preparation of bromomethylated poly(2,6-dimethyl-1,4-phenylene oxide) hollow fiber cation-exchange membranes and immobilization of cellulase thereon

Cited by 18 publications

References 32 publications

Suitability of magnetic nanoparticle immobilised cellulases in enhancing enzymatic saccharification of pretreated hemp biomass

Suitability of magnetic nanoparticle immobilised cellulases in enhancing enzymatic saccharification of pretreated hemp biomass

Physicochemical and Bioactive Properties of Soluble Dietary Fibers from Blasting Extrusion Processing (BEP)-Extruded Carrot Residues

Layered Co-Immobilization of β-Glucosidase and Cellulase on Polymer Film by Visible-Light-Induced Graft Polymerization

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