Preparation of Calcipotriol Emulsion Using Bacterial Exopolysaccharides as Emulsifier for Percutaneous Treatment of Psoriasis Vulgaris

Song, Bo; Song, Ruiteng; Cheng, Min; Chu, Hairong; Fang, Yan; Wang, Yuzhen

doi:10.3390/ijms21010077

Cited by 20 publications

(6 citation statements)

References 28 publications

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“…For coconut oil, emulsifying activity was similar at different pH levels for E24, but for E48, pH 5.1 was most effective, followed by pH 3.1; for E168, only pH 3.1 still presented some emulsification. Similar results were obtained with the EPS of the B. amyloliquefacens ZWJ strain [ 77 ], where the optimal concentration was 1.5 mg/L for two natural oils (olive oil (96.2%), sunflower oil (76%)). In the case of B. amyloliquefaciens LPL061 [ 21 ], the emulsifying activity was lower than that presented by EPS RT7 .…”

Section: Resultssupporting

confidence: 82%

Production and Characterisation of an Exopolysaccharide by Bacillus amyloliquefaciens: Biotechnological Applications

et al. 2023

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The Bacillus amyloliquefaciens RT7 strain was isolated from an extreme acidic environment and identified. The biodegradation capabilities of the strain using different carbon sources (glucose, oleic acid, Tween 80, PEG 200, and the combination of glucose–Tween 80) were evaluated via an indirect impedance technique. The glucose–Tween 80 combination was further studied using nuclear magnetic resonance (NMR). The exopolysaccharide (EPSRT7) that had been produced with the strain when biodegrading glucose–Tween 80 was isolated and characterised using different techniques (GC–MS, HPLC/MSMS, ATR–FTIR, TGA, and DSC), and its molecular weight was estimated. The results show that the average molecular weight of EPSRT7 was approximately 7.0794 × 104 Da and a heteropolysaccharide composed of mannose, glucose, galactose, and xylose (molar ratio, 1:0.5:0.1:0.1) with good thermostability. EPSRT7 showed good emulsifying activity against different natural oils and hydrocarbons at high concentrations (2 mg/mL) and at the studied pH range (3.1–7.2). It also presented good emulsifying activity compared to that of commercial emulsifiers. Lastly, EPSRT7 showed antioxidant capacity for different free radicals, a lack of cytotoxicity, and antioxidant activity at the cellular level. EPSRT7 has promising applications in bioremediation processes and other industrial applications.

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Section: Resultssupporting

confidence: 82%

Production and Characterisation of an Exopolysaccharide by Bacillus amyloliquefaciens: Biotechnological Applications

et al. 2023

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“…39 EPS is a natural nontoxic and non-irritating polysaccharide with good biocompatibility, which will facilitate meeting the requirements for product safety, patient compliance, and long-term treatment. 42 In addition, NYS can enhance the immune response to CA and protect the ultrastructure of the vaginal epithelium. 43 Above all, ENNE has certain advantages and strong application potential for the treatment of local fungal infections due to its nanoscale particle size, excellent mobility, pH stability, slow release, and biological adhesion.…”

Section: Discussionmentioning

confidence: 99%

<p>Ultrasound-Assisted Preparation of Exopolysaccharide/Nystatin Nanoemulsion for Treatment of Vulvovaginal Candidiasis</p>

Song¹,

Fang²,

Cheng³

et al. 2020

IJN

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Purpose: As one of the classic anti-Canidia albicans (CA) and vulvovaginal candidiasis (VVC) drugs, nystatin (NYS) is limited by poor water solubility and easy aggregation. Traditional NYS vaginal delivery formulations do not fully adapt to the specific environment of the vaginal cavity. The use of exopolysaccharides (EPS) has great application potential in emulsifiers, but its use has not been reported in nanoemulsions. In this work, an EPS/NYS nanoemulsion (ENNE) was developed to improve the activities of NYS against CA and VVC Methods: The ENNE was prepared by ultrasonic method using EPS as an emulsifier, liquid paraffin oil as an oil phase, PEG400 as a co-emulsifier, and NYS as the loaded drug. ENNE preparation was optimized by response surface method. After optimization, in vitro and in vivo analysis of the anti-CA activity; animal experiments; staining with propidium iodide (PI), periodic acid-schiff (PAS), and hematoxylin-eosin (H&E); and cytokine experiments were performed to investigate the therapeutic ability against VVC. Results: The optimal formulation and preparation parameters of ENNE were determined as follows: EPS content of 1.5%, PEG400 content of 3.2%, NYS content of 700 μg/mL, paraffin oil content of 5.0%, ultrasonic time of 15 min, and ultrasonic amplitude of 35%. The ENNE showed an encapsulated structure with an average particle size of 131.1 ± 4.32 nm. ENNE exhibited high storage and pH stability, as well as slow release. The minimum inhibitory concentration (MIC) of ENNE against CA was only 0.125 μg/mL and the inhibition zone was 19.0 ± 0.5 mm, for greatly improved anti-CA effect. The prepared ENNE destroyed the membrane of CA cells, and exhibited good anti-CA effect in vivo and therapeutic ability against VVC. Conclusion: The results of this study will promote the application of EPS in nanotechnology, which should lead to new and effective local drug formulations for treating VVC.

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“…They reported the ability of the reduced graphene oxide nanocarrier to the betterment of cyclosporine permeation, and retention in dermal layers could be used for better psoriasis management, with minimal adverse effects observed through oral/systemic routes [ 186 ]. Song et al loaded calcipotriol in exo-polysaccharides/calcipotriol (EPS/CPT) emulsion, sunflower oil, and calcipotriol as the loaded drug, and the therapeutic effect was evaluated [ 187 ]. The in vitro and in vivo studies expressed better efficacy for the management of psoriasis vulgaris by increasing the aggregation of CPT in psoriatic lesions and attenuating pro-inflammatory cytokine levels [ 187 ].…”

Section: Nanoscale Topical Pharmacotherapy In Effective Treatment Of ...mentioning

confidence: 99%

“…Song et al loaded calcipotriol in exo-polysaccharides/calcipotriol (EPS/CPT) emulsion, sunflower oil, and calcipotriol as the loaded drug, and the therapeutic effect was evaluated [ 187 ]. The in vitro and in vivo studies expressed better efficacy for the management of psoriasis vulgaris by increasing the aggregation of CPT in psoriatic lesions and attenuating pro-inflammatory cytokine levels [ 187 ].…”

Section: Nanoscale Topical Pharmacotherapy In Effective Treatment Of ...mentioning

confidence: 99%

Nanoscale Topical Pharmacotherapy in Management of Psoriasis: Contemporary Research and Scope

Ahmad

Mohammed

Algahtani

et al. 2022

JFB

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Psoriasis is a typical dermal condition that has been anticipated since prehistoric times when it was mistakenly implicit in being a variant of leprosy. It is an atypical organ-specific autoimmune disorder, which is triggered by the activation of T-cells and/or B-cells. Until now, the pathophysiology of this disease is not completely explicated and still, many research investigations are ongoing. Different approaches have been investigated to treat this dreadful skin disease using various anti-psoriatic drugs of different modes of action through smart drug-delivery systems. Nevertheless, there is no ideal therapy for a complete cure of psoriasis owing to the dearth of an ideal drug-delivery system for anti-psoriatic drugs. The conventional pharmacotherapy approaches for the treatment of psoriasis demand various classes of anti-psoriatic drugs with optimum benefit/risk ratio and insignificant untoward effects. The advancement in nanoscale drug delivery had a great impact on the establishment of a nanomedicine-based therapy for better management of psoriasis in recent times. Nanodrug carriers are exploited to design and develop nanomedicine-based therapy for psoriasis. It has a promising future in the improvement of the therapeutic efficacy of conventional anti-psoriatic drugs. The present manuscript aims to discuss the pathophysiology, conventional pharmacotherapy, and contemporary research in the area of nanoscale topical drug delivery systems for better management of psoriasis including the significance of targeted pharmacotherapy in psoriasis.

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Preparation of Calcipotriol Emulsion Using Bacterial Exopolysaccharides as Emulsifier for Percutaneous Treatment of Psoriasis Vulgaris

Cited by 20 publications

References 28 publications

Production and Characterisation of an Exopolysaccharide by Bacillus amyloliquefaciens: Biotechnological Applications

Production and Characterisation of an Exopolysaccharide by Bacillus amyloliquefaciens: Biotechnological Applications

<p>Ultrasound-Assisted Preparation of Exopolysaccharide/Nystatin Nanoemulsion for Treatment of Vulvovaginal Candidiasis</p>

Nanoscale Topical Pharmacotherapy in Management of Psoriasis: Contemporary Research and Scope

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