Preparation of chitosan/chondroitin sulfate complex microcapsules and application in controlled release of 5-fluorouracil

Huang, Liangliang; Sui, Weiping; Wang, Yuanxiu; Jiao, Qiang

doi:10.1016/j.carbpol.2009.11.007

Cited by 84 publications

(28 citation statements)

References 17 publications

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“…Studies have also investigated drug delivery systems based on Cs and chondroitin sulfate (ChS) molecules. Such reports include Ganza-Gonzales et al (50) , Sui et al (51) , and Huang et al (52) , which described the use of Cs-Chs-based microcapsules for the controlled release of heparin, metoclopramide and 5-fl uorouracil, respectively. The main advantage of this method is that it allows the production of delivery systems without the need for organic solvents in their formulations (30) .…”

Section: Optimized Nanoparticle Delivery Systems For Ampb Based On Chmentioning

confidence: 99%

New delivery systems for amphotericin B applied to the improvement of leishmaniasis treatment

Chávez-Fumagalli

Ribeiro

Castilho

et al. 2015

Rev. Soc. Bras. Med. Trop.

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Leishmaniasis is one of the six major tropical diseases targeted by the World Health Organization. It is a life-threatening disease of medical, social and economic importance in endemic areas. No vaccine is yet available for human use, and chemotherapy presents several problems. Pentavalent antimonials have been the drugs of choice to treat the disease for more than six decades; however, they exhibit high toxicity and are not indicated for children, for pregnant or breastfeeding women or for chronically ill patients. Amphotericin B (AmpB) is a second-line drug, and although it has been increasingly used to treat visceral leishmaniasis (VL), its clinical use has been hampered due to its high toxicity. This review focuses on the development and in vivo usage of new delivery systems for AmpB that aim to decrease its toxicity without altering its therapeutic efficacy. These new formulations, when adjusted with regard to their production costs, may be considered new drug delivery systems that promise to improve the treatment of leishmaniasis, by reducing the side effects and the number of doses while permitting a satisfactory cost-benefit rati

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Section: Optimized Nanoparticle Delivery Systems For Ampb Based On Chmentioning

confidence: 99%

New delivery systems for amphotericin B applied to the improvement of leishmaniasis treatment

Chávez-Fumagalli

Ribeiro

Castilho

et al. 2015

Rev. Soc. Bras. Med. Trop.

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“…17 Recently, PECs prepared from natural macromolecules have attracted attention for use in the development of drug-delivery systems, such as the complex between Cs and heparin, which have been formulated as vaccine adjuvants. 18 Some studies have investigated the drug-delivery systems based on Cs-ChS complexes, such as Huang et al, 19 Ganza-Gonzáles et al, 20 and Sui et al, 21 who have reported the use of Cs-ChS-based microcapsules for controlled release by 5-fluorouracyl, heparin, and metoclopramide, respectively, in their studies. Yeh et al characterized protein-loaded Cs-ChS nanoparticles using the PEC technique, 22 while Hu et al studied the influence of charge on fluorescein isothiocyanate (FITC)-bovine serum albuminloaded Cs-ChS nanoparticles upon cell uptake in human Caco-2 cell monolayers.…”

Section: Introductionmentioning

confidence: 99%

Novel targeting using nanoparticles: an approach to the development of an effective anti-leishmanial drug-delivery system

Ribeiro

Chávez-Fumagalli

Valadares

et al. 2014

IJN

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The study reported here aimed to develop an optimized nanoparticle delivery system for amphotericin B (AmpB) using a polyelectrolyte complexation technique. For this, two oppositely charged polymers presenting anti-leishmanial activity – chitosan (Cs) and chondroitin sulfate (ChS) – were used: Cs as a positively charged polymer and ChS as a negatively charged polymer. The chitosan (NQ) nanoparticles, chitosan-chondroitin sulfate (NQC) nanoparticles, and chitosan-chondroitin sulfate-amphotericin B (NQC-AmpB) nanoparticles presented a mean particle size of 79, 104, and 136 nm, respectively; and a polydispersity index of 0.2. The measured zeta potential of the nanoparticles indicated a positive charge in their surface, while scanning and transmission electron microscopy revealed spherical nanoparticles with a smooth surface. Attenuated total reflectance-Fourier transform infrared spectroscopy analysis showed an electrostatic interaction between the polymers, whereas the release profile of AmpB from the NQC-AmpB nanoparticles showed a controlled release. In addition, the Cs; ChS; and NQ, NQC, and NQC-AmpB nanoparticles proved to be effective against promastigotes of Leishmania amazonensis and Leishmania chagasi , with a synergistic effect observed between Cs and ChS. Moreover, the applied NQ, NQC, and NQC-AmpB compounds demonstrated low toxicity in murine macrophages, as well as null hemolytic activity in type O + human red blood cells. Pure AmpB demonstrated high toxicity in the macrophages. The results show that cells infected with L. amazonensis and later treated with Cs, ChS, NQ, NQC, NQC-AmpB nanoparticles, or pure AmpB presented with a significant reduction in parasite number in the order of 24%, 31%, 55%, 66%, 90%, and 89%, respectively. The data presented indicate that the engineered NQC-AmpB nanoparticles could potentially be used as an alternative therapy to treat leishmaniasis, mainly due its low toxicity to mammals’ cells.

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“…When these are repulsed, the minimum radius for endocytosis increases, therefore hindering endocytosis. 30 The ionic interaction between CT and some anionic polysaccharides, such as CS, 14,31 FC, 32 and heparin, 33,34 has recently been used to produce micro-(CS) or nanoparticles (heparin) with biological activity. The production of these micro-(CS) and nanostructured materials is feasible through ionic interactions among the polyelectrolytes, ie, the positive charges of CT at acid pH interacting with the negatively charged sulfated polysaccharides (interpolymer complexation).…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity

Silva¹,

Garcia²,

Mori³

et al. 2012

IJN

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Abstract:The aim of this study was to produce and characterize nanoparticles (NPs), combining chondroitin sulfate (CS) and fucoidan (FC) with chitosan for therapeutic purposes. These NPs were characterized by dynamic light scattering, zeta potential determination, and transmission electronic microscopy. The anticoagulant activity was determined for FC NPs and compared with FC solution at the same concentration. FC NPs showed regular shapes and better anticoagulant activity than free polysaccharide solution. FC solution did not affect coagulation compared to FC NPs, which increased up to two-fold, even at a lower concentration. Cytotoxicity and permeability tests were conducted using Caco-2 cell monolayer, exhibiting no toxic effect in this cell line and higher permeability for NP2 samples than FC solution at the same concentration.

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Preparation of chitosan/chondroitin sulfate complex microcapsules and application in controlled release of 5-fluorouracil

Cited by 84 publications

References 17 publications

New delivery systems for amphotericin B applied to the improvement of leishmaniasis treatment

New delivery systems for amphotericin B applied to the improvement of leishmaniasis treatment

Novel targeting using nanoparticles: an approach to the development of an effective anti-leishmanial drug-delivery system

Preparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity

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