Preparation of chitosan films mixed with superabsorbent polymer and evaluation of its haemostatic and antibacterial activities

Abstract

Blended and layered films were developed from chitosan and starch-poly (sodium acrylate-co-acrylamide) superabsorbant polymer (SAP) and were tested for haemostasis. The tensile properties of the films are reported. A simple in vitro test was used to peer rank the effect of the films on the ability of chitosan to aggregate blood cells. It was clear that the addition of SAP material to chitosan enhanced the ability of the resulted films to coagulate blood. When chitosan and SAP were blended with a ratio of 1 : 1 (v/v) using concentrations of 1-2% and 0.25-0.5% (v/v) of chitosan and SAP respectively, the resulted films reduced the erythrocyte sedimentation rate (ESR) by 22% as compared to the control, whereas the chitosan and SAP control films reduced ESR by 11% and 22% respectively. Also, the two layered films with 2% (v/v) chitosan exhibited the same percent of reduction. These haemostatic films were further investigated by FTIR, TGA, tensile, antimicrobial ability, and cytotoxicity. Some new peaks were observed by FTIR due to possible interaction between the AOH groups of the starch and ANH 3 þ groups of the chitosan. Also, the films showed good mechanical and thermal properties. Moreover, the films also expressed antibacterial activity against Pseudomonas aeruginosa with a bacterial reduction of 99%. All the film samples exhibited a viability percentage around 100% with no cytotoxic effect on the cells. Chitosan-SAP films can be described as biofilms with a homogeneous matrix, stable structure, and interesting mechanical properties, with possibilities of utilization in haemostasis.

Improving the fouling resistance of brackish water membranes via surface modification with graphene oxide functionalized chitosan

“…The high water flux of GO-based membranes is due to the frictionless GO carbon-rich surface [27,38], rather than the hydrophilic oxidized-rich region of GO [39]. Moreover, the linear polymeric Cs comprises β-1,4 glucosamine units, including amino and hydroxyl groups, which contribute towards the improvement of the membrane hydrophilicity [40][41][42][43].…”

Unusual Antibacterial Property of Mesoporous Titania Films: Drastic Improvement by Controlling Surface Area and Crystallinity

“…[8][9][10][16][17][18] Currently, new types of antibacterial films with enhanced activity have been fabricated by mixing with complex polymer-based materials or using special equipment, such as CVD. [32][33][34][35][36][37][38][39][40][41][42] In contrast, the present coating process based on sol-gel chemistry is very simple, cheap, and can be used on the industrial scale, which have opened up approaches for fabrication of new antibacterial materials and will be a key technology for solving current social problems in future.…”

An investigation of chitosan and its derivatives on red blood cell agglutination

“…21,22 The hemostatic properties of CS in solution are possibly due to its interaction with RBCs, which are linked together to establish a cellular clot or hemostatic plug. 23,24 In addition to a chitosan solution, several chitosan-based dressings have been produced such as lms, 25,26 porous sponges 27,28 and lyophilized bandages. [29][30][31] As the main component of these dressings, chitosan salts can improve the hemostatic efficacy by enriching the RBCs.…”