Preparation of CLEAs and magnetic CLEAs of a recombinant l-arabinose isomerase for d-tagatose synthesis

Sousa, Marylane de; Gurgel, Barbara Silva; Pessela, Benevides C.; Gonçalves, Luciana Rocha Barros

doi:10.1016/j.enzmictec.2020.109566

Cited by 23 publications

(8 citation statements)

References 29 publications

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“…It exhibited increased storage and operational stability and could be recycled several times with little loss of activity. 34 Higher losses observed at long reaction times could be due to dissolution of Fe 3 O 4 .…”

Section: Magnetisable Carriers and Cleas For Magnetic Separationmentioning

confidence: 99%

New frontiers in enzyme immobilisation: robust biocatalysts for a circular bio-based economy

Basso²,

2021

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Section: Magnetisable Carriers and Cleas For Magnetic Separationmentioning

confidence: 99%

New frontiers in enzyme immobilisation: robust biocatalysts for a circular bio-based economy

Basso²,

2021

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“…The immobilized l -AI exhibited a broader pH working range and can be used at higher temperature, ∼ 15 °C compared to free enzyme. Recently, the l -AI immobilized as cross-linked enzyme aggregates (CLEAs) using magnetic particles for cross-linking exhibited a 51-fold increase in stability under acidic conditions (pH 5.6) and high temperature (60 °C), as compared to the soluble enzyme . The magnetic cross-link increased the operational stability of the isomerase because around 70% of its initial activity could be retained after 9 cycles.…”

Section: Production Of Lignocellulosic Biomass-derived Productsmentioning

confidence: 99%

Enzymes, In Vivo Biocatalysis, and Metabolic Engineering for Enabling a Circular Economy and Sustainability

et al. 2021

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Since the industrial revolution, the rapid growth and development of global industries have depended largely upon the utilization of coal-derived chemicals, and more recently, the utilization of petroleum-based chemicals. These developments have followed a linear economy model (produce, consume, and dispose). As the world is facing a serious threat from the climate change crisis, a more sustainable solution for manufacturing, i.e., circular economy in which waste from the same or different industries can be used as feedstocks or resources for production offers an attractive industrial/business model. In nature, biological systems, i.e., microorganisms routinely use their enzymes and metabolic pathways to convert organic and inorganic wastes to synthesize biochemicals and energy required for their growth. Therefore, an understanding of how selected enzymes convert biobased feedstocks into special (bio)chemicals serves as an important basis from which to build on for applications in biocatalysis, metabolic engineering, and synthetic biology to enable biobased processes that are greener and cleaner for the environment. This review article highlights the current state of knowledge regarding the enzymatic reactions used in converting biobased wastes (lignocellulosic biomass, sugar, phenolic acid, triglyceride, fatty acid, and glycerol) and greenhouse gases (CO 2 and CH 4 ) into value-added products and discusses the current progress made in their metabolic engineering. The commercial aspects and life cycle assessment of products from enzymatic and metabolic engineering are also discussed. Continued development in the field of metabolic engineering would offer diversified solutions which are sustainable and renewable for manufacturing valuable chemicals.

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“…Various enzymes involved in biomass conversion, such as carbohydrases, lipases, proteases, and laccases, have been immobilized as m-CLEAs. , A striking example is the use of a m-CLEA of recombinant arabinose isomerase, with increased operational and storage stability as well as excellent recyclability, in the isomerization of d -galactose to the sweetener d -tagatose …”

Section: Recycling Of Enzymes As Heterogeneous Catalystsmentioning

confidence: 99%

Streamlining Design, Engineering, and Applications of Enzymes for Sustainable Biocatalysis

Sheldon

Brady

2021

ACS Sustainable Chem. Eng.

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In this Perspective we show how an expansion of the scope and impact of biocatalysis in industrial organic synthesis is enabled by streamlining the underpinning biocatalyst and bioprocess engineering. We begin by discussing how the underlying need for waste reduction and high (enantio)selectivities fostered the introduction of biocatalysis as a sustainable technology for the industrial synthesis of active pharmaceutical ingredients (APIs). We continue by showing how advances in molecular biology, in particular gene sequencing and protein engineering, enabled the development of more and better enzymes, thereby broadening the industrial scope of biocatalysis. Further process improvements are provided through protein engineering for enzyme immobilization and integration of enzyme production with in vivo immobilization. Finally, the use of immobilized enzymes in continuous operation (biocatalysis in flow) facilitates the sequential integration of multi-step reactions into enzymatic or chemo-enzymatic cascade processes, thus enabling the complete, cost-effective, and environmentally attractive production of APIs.

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Preparation of CLEAs and magnetic CLEAs of a recombinant l-arabinose isomerase for d-tagatose synthesis

Cited by 23 publications

References 29 publications

New frontiers in enzyme immobilisation: robust biocatalysts for a circular bio-based economy

New frontiers in enzyme immobilisation: robust biocatalysts for a circular bio-based economy

Enzymes, In Vivo Biocatalysis, and Metabolic Engineering for Enabling a Circular Economy and Sustainability

Streamlining Design, Engineering, and Applications of Enzymes for Sustainable Biocatalysis

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