Preparation of coculture system with three extracellular matrices using capillary force lithography and layer-by-layer deposition

Takahashi, Shintaro; Yamazoe, Hironori; Sassa, Fumihiro; Suzuki, Hiroaki; Fukuda, Junji

doi:10.1016/j.jbiosc.2009.06.013

Cited by 23 publications

(18 citation statements)

References 27 publications

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“…Recent work by Takahashi et al where they have adopted capillary force lithography to yield a wide range of coculture patterns to understand aspects of hepatocyte-fibroblast cocultures, such as existence of gap junctions, more sophisticatedly. 65 This demonstrates how parameters such as spatial distribution and orientation of cells, which are generally random during culturing, can now be well defined to carry out high-resolution optical analyses. A similar platform was developed much earlier to study hepatocyte and nonparenchymal cell interactions.…”

Section: Micropatterning In Coculture Tissue Engineeringmentioning

confidence: 95%

Coculture Strategies in Bone Tissue Engineering: The Impact of Culture Conditions on Pluripotent Stem Cell Populations

Janardhanan

Wang²,

Fisher³

2012

Tissue Engineering Part B: Reviews

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Section: Micropatterning In Coculture Tissue Engineeringmentioning

confidence: 95%

Coculture Strategies in Bone Tissue Engineering: The Impact of Culture Conditions on Pluripotent Stem Cell Populations

Janardhanan

Wang²,

Fisher³

2012

Tissue Engineering Part B: Reviews

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“…Control over cell adhesion was achieved by using thermally responsive polymers, which allowed maintenance of individual patterns while promoting confluent co-cultures. Other groups also have studied controlling heterotypic interactions in micropatterned co-cultures of rat hepatocytes and mouse fibroblasts, and reported that the liver function was preserved [76–79]. For instance, in order to find out the effects of heterotypic interactions on liver function, layered co-cultures of rat hepatocytes and human aortic endothelial cells (HAECs) were generated [68].…”

Section: Characterization Of Cell-cell Interactions In Patterned Cmentioning

confidence: 99%

Engineering systems for the generation of patterned co-cultures for controlling cell–cell interactions

Kaji

Camci‐Unal

Langer

et al. 2011

Biochimica et Biophysica Acta (BBA) - General Subjects

149

115

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Background-Inside the body, cells lie in direct contact or in close proximity to other cell types in a tightly controlled architecture that often regulates the resulting tissue function. Therefore, tissue engineering constructs that aim to reproduce the architecture and the geometry of tissues will benefit from methods of controlling cell-cell interactions with microscale resolution.Scope of the review-We discuss the use of microfabrication technologies for generating patterned co-cultures. In addition, we categorize patterned co-culture systems by cell type and discuss the implications of regulating cell-cell interactions in the resulting biological function of the tissues.Major conclusions-Patterned co-cultures are a useful tool for fabricating tissue engineered constructs and for studying cell-cell interactions in vitro, because they can be used to control the degree of homotypic and heterotypic cell-cell contact. In addition, this approach can be manipulated to elucidate important factors involved in cell-matrix interactions.General significance-Patterned co-culture strategies hold significant potential to develop biomimetic structures for tissue engineering. It is expected that they would create opportunities to develop artificial tissues in the future.

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“…Fibroblasts were then seeded onto collagen-coated free areas surrounding the hepatocyte islands. The resulting co-cultures produced albumin and induced hepatocyte cell -cell junction formation, neither of which occurred in monolayer cultures of hepatocytes (figure 1; Takahashi et al 2009). Several investigators have explored the effect of adhesion ligand patterns on cell behaviours.…”

Section: Nanoscale Surface Patterningmentioning

confidence: 99%

Engineering extracellular matrix through nanotechnology

Kelleher

Vacanti

2010

J. R. Soc. Interface.

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The goal of tissue engineering is the creation of a living device that can restore, maintain or improve tissue function. Behind this goal is a new idea that has emerged from twentieth century medicine, science and engineering. It is preceded by centuries of human repair and replacement with non-living materials adapted to restore function and cosmetic appearance to patients whose tissues have been destroyed by disease, trauma or congenital abnormality. The nineteenth century advanced replacement and repair strategies based on moving living structures from a site of normal tissue into a site of defects created by the same processes. Donor skin into burn wounds, tendon transfers, intestinal replacements into the urinary tract, toes to replace fingers are all examples. The most radical application is that of vital organ transplantation in which a vital part such as heart, lung or liver is removed from one donor, preserved for transfer and implanted into a patient dying of end-stage organ failure. Tissue engineering and regenerative medicine have advanced a general strategy combining the cellular elements of living tissue with sophisticated biomaterials to produce living structures of sufficient size and function to improve patients' lives. Multiple strategies have evolved and the application of nanotechnology can only improve the field. In our era, by necessity, any medical advance must be successfully commercialized to allow widespread application to help the greatest number of patients. It follows that business models and regulatory agencies must adapt and change to enable these new technologies to emerge. This brief review will discuss the science of nanotechnology and how it has been applied to this evolving field. We will then briefly summarize the history of commercialization of tissue engineering and suggest that nanotechnology may be of use in breeching the barriers to commercialization although its primary mission is to improve the technology by solving some remaining and vexing problems in its science and engineering aspects.

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Preparation of coculture system with three extracellular matrices using capillary force lithography and layer-by-layer deposition

Cited by 23 publications

References 27 publications

Coculture Strategies in Bone Tissue Engineering: The Impact of Culture Conditions on Pluripotent Stem Cell Populations

Coculture Strategies in Bone Tissue Engineering: The Impact of Culture Conditions on Pluripotent Stem Cell Populations

Engineering systems for the generation of patterned co-cultures for controlling cell–cell interactions

Engineering extracellular matrix through nanotechnology

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