Preparation of Complement Fixation Antigen of <i>Chlamydia psittaci</i> Grown in Tissue Culture by Treatment with β‐Propiolactone

Chiba, Nobuyuki; Takashima, Ikuo; Arikawa, Jiro; Hashimoto, Nobuo

doi:10.1111/j.1348-0421.1984.tb00785.x

Cited by 4 publications

(1 citation statement)

References 16 publications

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“…A booster injection was given 2 weeks after the first injection with the same mixture. 2 weeks later, Sarcoma 180 [8] cells were inoculated to produce ascitic fluid. Antibody protein was isolated from the immune ascitic fluid using an Affigel protein A column (Bio-Rad Laboratories, U.S.A.).…”

Section: Preparation Of Immune Spleen Cells and Immune Serummentioning

confidence: 99%

Protective immunity of Hantaan virus nucleocapsid and envelope protein studied using baculovirus-expressed proteins

Yoshimatsu

Yoo

Yoshida

et al. 1993

Archives of Virology

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Recombinant Hantaan virus nucleocapsid protein (rNP) and recombinant envelope (rEnv) proteins were prepared using a baculovirus expression system to examine the role of Hantaan virus structural proteins in protective immunity. Passive transfer of spleen cells from mice immunized with rNP conferred partial protection or prolongation of time to death from fatal Hantaan virus infection in suckling mice which were challenged with Hantaan virus at 40 LD50 (survival rate: 43%) or 4 LD50 (survival rate: 43%). The T cell-enriched fraction protected one mouse from lethal infection but the B cell-enriched fraction had no such effect on fatal HTN infection. The protective effects of the antibody against HTN challenge were examined by passive immunization. The monoclonal antibody ECO 2 directed to NP also conferred partial survival and significant difference in time to death. Although rEnv antigen failed to induce neutralizing antibody, both immune spleen cells and immune serum to rEnv conferred partial protection upon suckling mice. These results indicate that both nucleocapsid and envelope proteins of Hantaan virus were responsible for induction of cell mediated protective immunity. Vero E 6 cells infected with Hantaan virus expressed envelope protein on the surface, as determined by flow cytometry. However, there was only negligible expression of nucleocapsid protein.

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Section: Preparation Of Immune Spleen Cells and Immune Serummentioning

confidence: 99%

Protective immunity of Hantaan virus nucleocapsid and envelope protein studied using baculovirus-expressed proteins

Yoshimatsu

Yoo

Yoshida

et al. 1993

Archives of Virology

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Monoclonal Antibodies against Chlamydia psittaci

Toyofuku

Takashima

Arikawa

et al. 1986

Microbiology and Immunology

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Five monoclonal antibodies were prepared against Chlamydia (C.) psittaci strain Pigeon-1041 isolated from a feral pigeon in Sapporo. Reactions of these antibodies to chlamydiae were examined using five strains of C. psittaci and two strains of C. trachomatis in an enzyme-linked immunosorbent assay, microimmunofluorescent test and complement fixation test. The antibodies were divided into two groups : three genus-specific (A2, D2, and I21) and two strain-specific (F2 and H9) antibodies. The antigenic determinant site of A2 was KIO4 sensitive, but those of D2, F2, and H9 were not affected greatly by KIO4 treatment. Nine C. psittaci strains from feral pigeons and 16 strains from budgerigars were classified into three groups and four groups, respectively, by reaction patterns against the monoclonal antibodies.Chlamydia (C.) trachomatis strain were successfully classified into 15 serotypes using the microimmunofluorescent test (MIFT) by Wang et al (21) and Kuo et al (12). C. psittaci is distributed among numerous avian and mammalian species. However, serological classification of C. psittaci was achieved in strains from only limited host species by different serological tests (1, 8), and no standardized methods for classification have been developed. Preparation of antiserum with specificities and high titers against C. psittaci strains was not successful because of the high virulence to laboratory animals and also common antigenicity among the strains.Advances in monoclonal antibody technique have made the antigenic analysis of chlamydia possible. Antigenic analysis of C. trachomatis using monoclonal antibodies with different specificities revealed that the organisms had a wide variety of antigens each specific to genus, species, subspecies, and type (20). The chemical nature of these antigens was also investigated (2, 13). However, no reports have been published on monoclonal antibodies against C. psittaci.In this report, monoclonal antibodies were prepared against a C. psittaci strain isolated from a feral pigeon in Japan in 1982, and the reactivities of the antibodies to several strains of C. psittaci and C. trachomatis were analyzed. These monoclonal antibodies were also applied for serological grouping of C. psittaci strains newly isolated from pigeons and budgerigars.

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Serological assessment of chlamydial infection in the koala by a slide EIA technique

et al. 1991

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A rapid and simplified slide enzyme immunosorbent assay (EIA) was developed for the diagnosis of chlamydial infection in the koala. HeLa 229 cells infected with koala strain Chlamydia psittaci were fixed on the surface of multiwell slides and used as the antigen. The assay consisted of first reacting koala antiserum with the fixed C psittaci antigen, followed by reaction with biotinylated rabbit anti-koala IgG, ABC reagent and substrate. The chlamydial EIA antibody titres obtained were compared with those of a complement fixation (CF) test using koala strain C psittaci as antigen. Of 35 koala sera tested, 16 CF positive sera (greater than or equal to 1:8) also had a positive titre (greater than or equal to 1:200) in the slide EIA test (sensitivity 93.8%, 15/16). Nineteen CF negative sera were also negative in the slide EIA (specificity 100%, 19/19). Sixty-eight samples of koala blood were collected by ear-prick using a sampling paper method and were assayed by both tests. Sensitivity of the slide EIA was 100% (15/15) and specificity of the test was 96.2% (51/53). To simplify the slide EIA for use as a practical screening test, a 3-point serum dilution series (1:100, 1:200, 1:400) was used. This 3-point slide EIA was compared with the CF test using sheep strain chlamydial antigen. Thirty-nine sera were assayed by both tests. The sensitivity of the 3-point method was 85.7% (6/7) and the specificity was 71.9% (23/32) as compared with the sheep antigen CF test.(ABSTRACT TRUNCATED AT 250 WORDS)

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Preparation of Complement Fixation Antigen of Chlamydia psittaci Grown in Tissue Culture by Treatment with β‐Propiolactone

Cited by 4 publications

References 16 publications

Protective immunity of Hantaan virus nucleocapsid and envelope protein studied using baculovirus-expressed proteins

Protective immunity of Hantaan virus nucleocapsid and envelope protein studied using baculovirus-expressed proteins

Monoclonal Antibodies against Chlamydia psittaci

Serological assessment of chlamydial infection in the koala by a slide EIA technique

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