Preparation of Curcubit[6]uril functionalized CuO Nanoparticles: A New Nanosensing Scheme Based on Fluorescence recovery after FRET for the Label Free Determination of Dopamine

Abstract

In this manuscript, the aim is to functionalize CuO nanoparticles with the supramolecular host molecule, in this case curcubit[6]uril to have a host guest interaction between the CuO nanoparticles and probe (guest) molecules. Acridine Orange (AO) is applied as a guest molecule. Acridine orange binds with curcubit[6]uril via its carbonyl rims. In the presence of curcubit[6]uril conjugated CuO nanoparticles, the fluorescence intensity of AO is quenched by the CuO NPs through fluorescence resonance energy transfer phenomenon. However, dopamine adsorbs on the surface of CuO nanoparticles by getting incorporated into CB[6] host molecules and kicks out AO from curcubit[6]uril pocket, thus, discouraging fluorescence resonance energy transfer phenomenon and thus enhancing fluorescence intensity of AO by a factor of 3. This fluorescence recovery has been utilized to design a new detection system for dopamine. The assay, measured at fluorescence excitation and emission wavelengths of 480 nm and 520 nm respectively, works in the 0-40 μM concentration range of dopamine with 40 nM limit of detection. This method is not interfered by ascorbic acid, uric acid, glucose, tryptophan and acetaminophen. The proposed method provides a good recovery from synthetic samples and shows good stability.

An Unsymmetrical Perylene Diimide Dye Modified Carbon Felt Electrode as A Novel Electrochemical Platform for Dopamine Detection

“…[1] Also, higher amounts of dopamine in the body may be an indicator for diseases like Alzheimer's, Parkinson's, schizophrenia, epilepsy and attention deficit hyperactivity disorder. [1][2][3][4] It has also been known that this molecule is in cationic form and can be found in the body fluids and brain tissues. [1] Besides being a neurotransmitter, dopamine can also be used as medicine for treatment i. e. of bradycardia and hypotension.…”

“…[1] Besides being a neurotransmitter, dopamine can also be used as medicine for treatment i. e. of bradycardia and hypotension. [3,5] As a result, due to its importance in health, detection of dopamine is crucial and many attempts have been made in order to determine it properly. Up to now, fluorimetric, chromatographic, electrophoretic and spectroscopic methods have been reported in the literature for the detection of dopamine.…”

“…[1] For overwhelming these drawbacks, new strategies like centri-voltammetry [7] have been developed or various electrode modification techniques have been applied. [1][2][3][4]6,8] Perylene and perylene derivatives have been known to possess enhanced absorption coefficient, high fluorescence quantum yield and better stability in terms of chemical, thermal and photochemical reactions. The other significant property of perylene derivatives is their high charge transfer properties which pave the way for enhanced electrochemical properties.…”

“…Dopamine is a neurotransmitter that has fundamental aspects in central nervous, cardiovascular and hormonal systems . Also, higher amounts of dopamine in the body may be an indicator for diseases like Alzheimer's, Parkinson's, schizophrenia, epilepsy and attention deficit hyperactivity disorder . It has also been known that this molecule is in cationic form and can be found in the body fluids and brain tissues .…”

“…It has also been known that this molecule is in cationic form and can be found in the body fluids and brain tissues . Besides being a neurotransmitter, dopamine can also be used as medicine for treatment i. e. of bradycardia and hypotension . As a result, due to its importance in health, detection of dopamine is crucial and many attempts have been made in order to determine it properly.…”