Preparation of curcumin loaded poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) nanofibers and their in vitro antitumor activity against Glioma 9L cells

Guo, Gang; Fu, Shaozhi; Zhou, Liangxue; Liang, Hang; Fan, Min; Luo, Feng; Zhang, Qian; Wei, Yuquan

doi:10.1039/c1nr10484e

Cited by 148 publications

(84 citation statements)

References 34 publications

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“…Moreover, the DL capacity and entrapment efficiency were in the range of 9.1-9.3% and 90-94%, respectively. The prepared PCEC nanoparticles showed no cytotoxicity on A549 cells in vitro and in vivo in nude mice, demonstrating that PCEC nanocarriers were safe as drug delivery system as many studies reported (Guo et al, 2011). GEF was released in a controlled manner from the nanoparticles, but in vitro might lead to a weaker antitumor effect because of the lack of cumulative amount of drug slowly released and EPR effect.…”

Section: Discussionmentioning

confidence: 92%

In vitro and in vivo antitumor effect of gefitinib nanoparticles on human lung cancer

Chen

Zhang

et al. 2017

Drug Delivery

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Gefitinib (GEF) is the first epidermal growth factor receptor (EGFR)-targeting agent launched as an anticancer drug. It is an accepted opinion that modifying GEF strong hydrophobicity and poor bioavailability would not only enhance its antitumor effects, but also reduce its side effects. In this study, GEF-loadedpoly(e-caprolactone)-poly(ethyleneglycol)-poly(e-caprolactone) (PCEC) -bearing nanoparticles (GEF-NPs) were prepared by a solid dispersion method and characterized. The particle sizes increased with the increase in GEF/PCEC mass ratio in feed. GEF-NPs (10%) were mono-dispersed, smaller than 24 nm, zeta potential was approximately À18 mV, percentage encapsulation and loading, were more than 9% and 92%, respectively, and drug was slowly released but without a biphasic pattern. Microscopy studies of the optimized formulation confirmed that the prepared nanoparticles are spherical in nature. Cytotoxicity results indicated that cell growth inhibition induced by free GEF and GEF-NPs were dose and time dependent. Compared with free GEF, GEF-NPs enhanced antitumor effects, reduced side effects and significantly prolonged survival time in vivo. CD31, ki-67 and EGFR expression were significantly lower in the GEF-NPs group compared with other groups (p< .05). These findings demonstrated that GEF-NPs have the potential to attain superior outcomes and to overcome complications such as organs toxicity, therapeutic resistance and disease relapse. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 92%

In vitro and in vivo antitumor effect of gefitinib nanoparticles on human lung cancer

Chen

Zhang

et al. 2017

Drug Delivery

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“…The emerging applications of nanotechnology-based cancer therapy provide a potent platform to improve poor water solubility and bioavailability of hydrophobic antitumor agents. 12,13 Recently, nanoparticle (NP)-based anticancer drug delivery systems, especially drug formulations with biodegradable polymeric NPs, have attracted considerable attention for their numerous advantages such as high cellular uptake, enhanced permeability and retention effect, and reduced cancer cell drug resistance. [14][15][16][17] Among the US Food and Drug Administration (FDA)-approved biodegradable polymers, poly(ε-caprolactone) (PCL) has gained considerable interest for drug delivery.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of genistein-loaded biodegradable TPGS-b-PCL nanoparticles for improved therapeutic effects in cervical cancer cells

Zhang¹,

Gan²,

Zeng³

et al. 2015

IJN

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Genistein is one of the most studied isoflavonoids with potential antitumor efficacy, but its poor water solubility limits its clinical application. Nanoparticles (NPs), especially biodegradable NPs, entrapping hydrophobic drugs have promising applications to improve the water solubility of hydrophobic drugs. In this work, TPGS- b -PCL copolymer was synthesized from ε-caprolactone initiated by d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) through ring-opening polymerization and characterized by Fourier transform infrared spectroscopy, proton nuclear magnetic resonance spectroscopy, gel permeation chromatography, and thermogravimetric analysis. The genistein-loaded NPs were prepared by a modified nanoprecipitation method and characterized in the aspects of particle size, surface charge, morphology, drug loading and encapsulation efficiency, in vitro drug release, and physical state of the entrapped drug. The TPGS- b -PCL NPs were found to have higher cellular uptake efficiency than PCL NPs. MTT and colony formation experiments indicated that genistein-loaded TPGS- b -PCL NPs achieved the highest level of cytotoxicity and tumor cell growth inhibition compared with pristine genistein and genistein-loaded PCL NPs. Furthermore, compared with pristine genistein and genistein-loaded PCL NPs, the genistein-loaded TPGS- b -PCL NPs at the same dose were more effective in inhibiting tumor growth in the subcutaneous HeLa xenograft tumor model in BALB/c nude mice. In conclusion, the results suggested that genistein-loaded biodegradable TPGS- b -PCL nanoparticles could enhance the anticancer effect of genistein both in vitro and in vivo, and may serve as a potential candidate in treating cervical cancer.

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“…This result, together with the mixed results obtained from the scratch test, indicated that C1 might be at the threshold of being toxic for NHDFs. Therefore, although such concentrations might be attractive for anti-cancer therapies 35 or antimicrobial applications, 24 they are unlikely to be encouraging tissue regeneration. These results are consistent with previously published data, showing that curcumin inhibits cell growth or induces apoptosis in a variety of cell lines, including fibroblasts, at concentrations ranging from 10 to 100 μM.…”

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confidence: 99%

“…35 It was shown that the incorporation of curcumin actually increases the conductivity of electrospinning solution, thereby enhancing the thinning of the spinning jet and decreasing the fiber diameter. 22 Mechanical, chemical, and thermal properties of filaments Mechanical properties Figure 3A and B shows the mechanical properties of filaments.…”

mentioning

confidence: 99%

Investigating the use of curcumin-loaded electrospun filaments for soft tissue repair applications

Mouthuy¹,

Škoc²,

Gašparović³

et al. 2017

IJN

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Electrospun filaments represent a new generation of medical textiles with promising applications in soft tissue repair. A potential strategy to improve their design is to combine them with bioactive molecules. Curcumin, a natural compound found in turmeric, is particularly attractive for its antioxidant, anti-inflammatory, and antimicrobial properties. However, investigating the range of relevant doses of curcumin in materials designed for tissue regeneration has remained limited. In this paper, a wide range of curcumin concentrations was explored and the potential of the resulting materials for soft tissue repair applications was assessed. Polydioxanone (PDO) filaments were prepared with various amounts of curcumin: 0%, 0.001%, 0.01%, 0.1%, 1%, and 10% (weight to weight ratio). The results from the present study showed that, at low doses (≤0.1%), the addition of curcumin has no influence on the spinning process or on the physicochemical properties of the filaments, whereas higher doses lead to smaller fiber diameters and improved mechanical properties. Moreover, filaments with 0.001% and 0.01% curcumin stimulate the metabolic activity and proliferation of normal human dermal fibroblasts (NHDFs) compared with the no-filament control. However, this stimulation is not significant when compared to the control filaments (0%). Highly dosed filaments induce either the inhibition of proliferation (with 1%) or cell apoptosis (with 10%) as a result of the concentrations of curcumin found in the medium (9 and 32 μM, respectively), which are near or above the known toxicity threshold of curcumin (~10 μM). Moreover, filaments with 10% curcumin increase the catalase activity and glutathione content in NHDFs, indicating an increased production of reactive oxygen species resulting from the large concentration of curcumin. Overall, this study suggested that PDO electrospun filaments loaded with low amounts of curcumin are more promising compared with higher concentrations for stimulating tissue repair. This study also highlighted the need to explore lower concentrations when using polymers as PDO, such as those with polycaprolactone and other degradable polyesters.

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Preparation of curcumin loaded poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) nanofibers and their in vitro antitumor activity against Glioma 9L cells

Cited by 148 publications

References 34 publications

In vitro and in vivo antitumor effect of gefitinib nanoparticles on human lung cancer

In vitro and in vivo antitumor effect of gefitinib nanoparticles on human lung cancer

Fabrication of genistein-loaded biodegradable TPGS-b-PCL nanoparticles for improved therapeutic effects in cervical cancer cells

Investigating the use of curcumin-loaded electrospun filaments for soft tissue repair applications

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