Preparation of dummy molecularly imprinted polymers for extraction of Zearalenone in grain samples

Huang, Zhipeng; He, Juan; Li, Yuanyuan; Wu, Chaojun; You, Long; Wei, Hongliang; Li, Kai; Zhang, Shusheng

doi:10.1016/j.chroma.2019.05.022

Cited by 46 publications

(26 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The template leakage would yield false‐positive results and result in the failure of analysis . In order to solve these problems, dummy MIPs were developed and have been proved the effectiveness . Here, we aimed to prepare PA‐MIPs‐SPME for the specific recognition of toxic PAs from herbal medicine.…”

Section: Resultsmentioning

confidence: 99%

Molecularly imprinted polymer solid‐phase microextraction coupled with ultra high performance liquid chromatography and tandem mass spectrometry for rapid analysis of pyrrolizidine alkaloids in herbal medicine

Luo

Chen

et al. 2019

J of Separation Science

View full text Add to dashboard Cite

Pyrrolizidine alkaloids are the most widely distributed natural toxins, and pyrrolizidine alkaloid‐containing herbal medicines are probably the most common poisonous plants affecting humans. We reported pyrrolizidine alkaloid‐molecularly imprinted polymer solid‐phase microextraction for the selective adsorption of toxic pyrrolizidine alkaloids from herbal medicine. A sulfonic compound, sodium allylsulfonate, was chosen as the functional monomer to interact with pyrrolizidine alkaloids through strong ionic interaction. To avoid template leakage and for the aim of cost saving, a relatively cheap dummy template was used for the fabrication of molecularly imprinted polymer‐solid‐phase microextraction fibers. The obtained fibers showed selective adsorption ability for four pyrrolizidine alkaloids, including europine, echimidine, lasiocarpine, and heliotrine. The extraction parameters, such as extraction time, extraction temperature, shaking speed, elution solvent and elution time, were optimized. Then ultra high performance liquid chromatography with mass spectrometry coupled with molecularly imprinted polymer‐solid‐phase microextraction method was developed for the fast and efficient analysis of four pyrrolizidine alkaloids from the model herbal plant Farfarae Flos. The established method was validated and exhibited satisfactory accuracy and precision. The present method provides an innovative and fast analytical strategy for the determination of trace toxic pyrrolizidine alkaloids in complicated samples.

show abstract

Section: Resultsmentioning

confidence: 99%

Molecularly imprinted polymer solid‐phase microextraction coupled with ultra high performance liquid chromatography and tandem mass spectrometry for rapid analysis of pyrrolizidine alkaloids in herbal medicine

Luo

Chen

et al. 2019

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…As MIPs for toxins were developed later than ISs, the latter are then considered as selective sorbents of reference and some results obtained with MIP were also compared with those obtained with ISs. In most of the cases, this evaluation consisted of comparing the cleaning effect on the baseline of chromatograms obtained by LC analysis after extraction with the two types of sorbent [144,145,157]. The results were similar for all these studies with a slightly higher purification effect obtained with ISs, which eliminate more interferents and thus lead to a cleaner baseline than with MIP.…”

Section: Molecularly Imprinted Polymersmentioning

confidence: 99%

“…Nevertheless, the latter remains effective since no interferent was co-eluted with the toxin of interest. An example of comparison is given in Figure 2 that corresponds to the LC-Fluo analysis of a corn extract purified on a commercially available IS (Figure 2A) or on MIP ( Figure 2B) [157]. As mentioned by the authors, the impurity peaks in the chromatogram corresponding to the purification on the IS were significantly lower than those observed on the chromatogram obtained when using the MIP, but the peak height of ZON in the two chromatograms did not differ so much.…”

Section: Molecularly Imprinted Polymersmentioning

confidence: 99%

See 1 more Smart Citation

Immunoaffinity Extraction and Alternative Approaches for the Analysis of Toxins in Environmental, Food or Biological Matrices

Delaunay

Combès

Pichon

2020

Toxins

View full text Add to dashboard Cite

The evolution of instrumentation in terms of separation and detection allowed a real improvement of the sensitivity and analysis time. However, the analysis of ultra-traces of toxins in complex samples requires often a step of purification and even preconcentration before their chromatographic analysis. Therefore, immunoaffinity sorbents based on specific antibodies thus providing a molecular recognition mechanism appear as powerful tools for the selective extraction of a target molecule and its structural analogs to obtain more reliable and sensitive quantitative analysis in environmental, food or biological matrices. This review focuses on immunosorbents that have proven their efficiency in selectively extracting various types of toxins of various sizes (from small mycotoxins to large proteins) and physicochemical properties. Immunosorbents are now commercially available, and their use has been validated for numerous applications. The wide variety of samples to be analyzed, as well as extraction conditions and their impact on extraction yields, is discussed. In addition, their potential for purification and thus suppression of matrix effects, responsible for quantification problems especially in mass spectrometry, is presented. Due to their similar properties, molecularly imprinted polymers and aptamer-based sorbents that appear to be an interesting alternative to antibodies are also briefly addressed by comparing their potential with that of immunosorbents.

show abstract

“…The technology is being gradually applied to the pretreatment of trace toxic and harmful substances in food, owing to their features of special selectivity, high tolerance, thermal stability, low production cost, and easy mass production. These attributes can make up for the deficiency of traditional SPE . In a recent work by Szekely et al., MIPs were synthesized by suspension polymerization .…”

Section: Introductionmentioning

confidence: 99%

Combined biocompatible medium with molecularly imprinted polymers for determination of aflatoxins B1 in real sample

Song

Chen

et al. 2019

J of Separation Science

Self Cite

View full text Add to dashboard Cite

A kind of molecularly imprinted polymers modified with biocompatible medium was prepared by suspension polymerization. The obtained hybrid materials were used as the adsorbents for the solid‐phase extraction of aflatoxins B1 in real samples. A structural analog of the target, 6‐methyl‐4‐phenylchroman‐2‐one was used as the pseudo‐template, owing to their lower toxicity and cheaper price compared with aflatoxins B1; and methacrylic acid and glycidyl methacrylate were used as the co‐monomers. Scanning electron microscopy and size distribution analysis were used to characterize the obtained polymers. The extraction parameters were optimized to achieve the desired extraction performance. The polymer solid‐phase extraction coupled with high‐performance liquid chromatography was successfully applied to determine aflatoxins B1 from soy sauce without the process of protein removal. Under the optimum extraction conditions, the detection results of aflatoxins B1 in lab‐made column in soy sauce samples was carried out, with a recovery rate of 96%. The established method presented a linear range from 10 to 1000 ng/mL with the coefficient of determination of 0.9994 and the limit of detection of 0.05 ng/mL. Likewise, the inherent selectivity of lab‐made column towards aflatoxins B1, Ochratoxin A, and Zearalenone was demonstrated.

show abstract

Preparation of dummy molecularly imprinted polymers for extraction of Zearalenone in grain samples

Cited by 46 publications

References 44 publications

Molecularly imprinted polymer solid‐phase microextraction coupled with ultra high performance liquid chromatography and tandem mass spectrometry for rapid analysis of pyrrolizidine alkaloids in herbal medicine

Molecularly imprinted polymer solid‐phase microextraction coupled with ultra high performance liquid chromatography and tandem mass spectrometry for rapid analysis of pyrrolizidine alkaloids in herbal medicine

Immunoaffinity Extraction and Alternative Approaches for the Analysis of Toxins in Environmental, Food or Biological Matrices

Combined biocompatible medium with molecularly imprinted polymers for determination of aflatoxins B1 in real sample

Contact Info

Product

Resources

About