Preparation of ELISA and Lateral Flow Kits for rapid Diagnosis of Mycoplasma gallisepticum in Poultry

El-Yazeed, Heidy Abo; Al-Atfeehy, Nayera M.; Abotaleb, Rasha; Sayed, Rafik Hamed; Marouf, Sherif

doi:10.1038/s41598-020-65848-7

Cited by 8 publications

(5 citation statements)

References 16 publications

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“…The evaluation of the relative specificity, sensitivity, and accuracy for LFIT using P04310-10 IDEXX brucellosis ovine/caprine Ab ELISA test (gold standard) was performed, and the results of sensitivity, specificity, and accuracy of LFIT and Rose Bengal test were compared with those of ELISA and found to be 74%, 89%, and 81% and 59%, 76.9%, and 66%, respectively. A nearly similar result was obtained by Elyazeed et al [27], who found that the LFIT had low sensitivity with high specificity (77.5% and 92%), respectively, for the detection of Mycoplasma gallisepticum. For the detection of brucellosis, the sensitivity and specificity were recorded as 78.57% and 93.07%, respectively [28].…”

Section: Discussionsupporting

confidence: 84%

Preparation and evaluation of a lateral flow immunochromatographic nanogold diagnostic kit for brucellosis in sheep

et al. 2022

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Background and Aim: Brucellosis is a zoonotic disease with a worldwide distribution. It has a serious impact on the health of humans and animals, along with a negative impact on the economy. This study aimed to prepare and evaluate the diagnostic performance of a lateral flow immunochromatographic test (LFIT) nanogold diagnostic kit for detecting brucellosis in sheep. Materials and Methods: A rapidly developed LFIT, in which lipopolysaccharide conjugates with nanogold molecules, was placed on the conjugate pad. One hundred ovine serum samples were tested to detect Brucella antibodies (Ab) using the prepared lateral flow immunochromatography assay (LFA) kit and Rose Bengal test. The evaluation of specificity, sensitivity, and accuracy for LFIT and Rose Bengal plate test was conducted using the P04310-10 IDEXX brucellosis ovine/ caprine Ab enzyme-linked immunosorbent assay (ELISA) test (gold standard). Results: The lower amount of Brucella Ab in the ovine serum samples was detected and was 1.58 S/P ratio ELISA titer/100 μL using LFIT and with Rose Bengal to detect 1.86 S/P ratio ELISA. The results showed that the developed LFIT had high specificity with no cross-reactivity with other tested bacteria. The calculated sensitivity, specificity, and accuracy of LFIT and Rose Bengal test using the P04310-10 IDEXX brucellosis ovine/caprine Ab ELISA test (gold standard) were 74% and 89%, 81% and 59%, and 76.9% and 66%, respectively. Conclusion: The present results showed interesting results implying that the LFIA strip test could be used as a substantial diagnostic tool for field screening ovine Brucella as an essential step in the control of brucellosis. However, further studies for the validation of the present findings are necessary.

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Section: Discussionsupporting

confidence: 84%

Preparation and evaluation of a lateral flow immunochromatographic nanogold diagnostic kit for brucellosis in sheep

et al. 2022

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“…Mycoplasmas are small prokaryotes that lack a cell wall, but they are surrounded by 3 layers of the plasma membrane, 300 to 800 nm in diameter. It can be detected in humans and animals, with an optimum growth temperature of 37°C with the ability to replicate outside of host cells ( Brown et al., 2007 ; Abdel Halium et al., 2019 ; Abo Elyazeed et al., 2020 ; Sawicka et al., 2020 ). Mycoplasma gallisepticum is the most virulent and economically significant bacterial respiratory infection of poultry.…”

Section: Introductionmentioning

confidence: 99%

Mycoplasma gallisepticum: a devastating organism for the poultry industry in Egypt

et al. 2022

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Mycoplasma gallisepticum ( MG ) is a worldwide ruined bacteria affecting different avian species, causing severe economic losses. Consequently, the current research sought to detect the incidence of MG among different commercial broiler, layer chickens and turkey farms, and environmental litter samples in different Egyptian governorates (Damietta, Giza, El-Qalyobia, El-Sharqia, and El-Behera) from January 2019 to December 2020. Four hundred samples (infraorbital sinus aspirates, tracheal swabs, serum from diseased birds, and organ samples; lung tissues, air sacs and tracheal bifurcation from freshly dead birds), and environmental samples (litter) were collected for MG isolation. Samples were subjected to phenotypic and molecular identification. Positive bacteriological samples were subjected for molecular identification using polymerase chain reaction ( PCR ) test to detect MG, then sequencing for PCR amplicon of mgc 2 gene. Out of 332 samples subjected for bacteriological examination, 206 were bacteriologically positive for MG with an incidence of 62%. The highest incidence of MG was detected in turkey farms at a rate of 83%, followed by broiler chicken farms, layer chicken farms and litter samples at a percentage of 70, 40, and 40, respectively. The highest prevalence of MG in chickens and turkey was recorded during the winter and autumn seasons. Molecular identification of MG isolates revealed that 85% of isolates were positive for mgc 2 gene using PCR. The Four sequenced strains in this study are closely related and placed in one group with the vaccine strain 6/85 and ts11 strain.

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“…The sample is placed on the testing device, and results are displayed within 5-30 minutes, making it easy to detect specific analytes in fluids or other samples (Gao et al, 2018;Koczula and Gallotta, 2016). Elyazeed et al (2020) conducted a study to develop, assess, and compare LFAs with commercial ELISA kits and PCR methods. The research indicated that lateral flow assays demonstrated lower sensitivity compared to PCR but higher specificity.…”

Section: Lateral Flow Immunochromatographic Analysismentioning

confidence: 99%

Mycoplasma galliscepticum: An overview

Ruizhi,

Xi,

Huiqi

et al. 2024

Afr. J. Microbiol. Res.

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The prokaryotic microorganism Mycoplasma gallisepticum (MG) belongs to the phylum Firmicutes, the order Mycoplasmatales, the family Mycoplasmataceae, and the genus Mycoplasma. MG parasitizes the respiratory tract of various poultry species, causing a range of symptoms from indistinct features to tracheitis and air sacculitis. Common signs include respiratory tract infection indicators such as coughing, asthma, a runny nose and rales. Both domestic and international reports underscore MG's transmission and infection capacity, signifying its global importance as a source of poultry infection. Further research is imperative to grasp its impact on both farmed and wild animals. This article comprehensively reviews current research progress on MG, encompassing its biological characteristics, infection traits, pathogenic mechanisms, epidemiology, detection methods, treatment, and control measures. It aims to serve as a reference for comprehensive prevention and control strategies against MG in the future.

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Preparation of ELISA and Lateral Flow Kits for rapid Diagnosis of Mycoplasma gallisepticum in Poultry

Cited by 8 publications

References 16 publications

Preparation and evaluation of a lateral flow immunochromatographic nanogold diagnostic kit for brucellosis in sheep

Preparation and evaluation of a lateral flow immunochromatographic nanogold diagnostic kit for brucellosis in sheep

Mycoplasma gallisepticum: a devastating organism for the poultry industry in Egypt

Mycoplasma galliscepticum: An overview

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