Preparation of Ethanol Extract of Propolis Loaded Niosome Formulation and Evaluation of Effects on Different Cancer Cell Lines

Çetin, Emel Öykü; Salmanoğlu, Derya Selcen; Ozden, Ilknur; Ors-Kumoglu, Gizem; Akar, Sibel; Demirozer, Melis; Karabey, Fatih; Kılıç, Kubilay Doğan; Kirilmaz, Levent; Uyanıkgil, Yiğit; Sevimli-Gur, Canan

doi:10.1080/01635581.2021.1876889

Cited by 12 publications

(4 citation statements)

References 62 publications

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“…The efficacy to entrap the EEP was different, as indicated by the %EE and the total phenolic content of EEP. In this study, the highest level of EE was gained with the F2 formulation, of approximately 80%, which is a higher gain in EE than in other previous works [ 15 , 51 ]. This effect might be due to the longer alkyl chain length of Span 60 (Span 60 > Span 40 > Span 20), as the bilayer expansion restricts the EEP in the structure [ 48 ].…”

Section: Discussioncontrasting

confidence: 46%

Nano-Delivery System of Ethanolic Extract of Propolis Targeting Mycobacterium tuberculosis via Aptamer-Modified-Niosomes

et al. 2023

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Tuberculosis (TB) therapy requires long-course multidrug regimens leading to the emergence of drug-resistant TB and increased public health burden worldwide. As the treatment strategy is more challenging, seeking a potent non-antibiotic agent has been raised. Propolis serve as a natural source of bioactive molecules. It has been evidenced to eliminate various microbial pathogens including Mycobacterium tuberculosis (Mtb). In this study, we fabricated the niosome-based drug delivery platform for ethanolic extract of propolis (EEP) using thin film hydration method with Ag85A aptamer surface modification (Apt-PEGNio/EEP) to target Mtb. Physicochemical characterization of PEGNio/EEP indicated approximately −20 mV of zeta potential, 180 nm of spherical nanoparticles, 80% of entrapment efficiency, and the sustained release profile. The Apt-PEGNio/EEP and PEGNio/EEP showed no difference in these characteristics. The chemical composition in the nanostructure was confirmed by Fourier transform infrared spectrometry. Apt-PEGNio/EEP showed specific binding to Mycobacterium expressing Ag85 membrane-bound protein by confocal laser scanning microscope. It strongly inhibited Mtb in vitro and exhibited non-toxicity on alveolar macrophages. These findings indicate that the Apt-PEGNio/EEP acts as an antimycobacterial nanoparticle and might be a promising innovative targeted treatment. Further application of this smart nano-delivery system will lead to effective TB management.

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Section: Discussioncontrasting

confidence: 46%

Nano-Delivery System of Ethanolic Extract of Propolis Targeting Mycobacterium tuberculosis via Aptamer-Modified-Niosomes

et al. 2023

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“…For MCF-7 cells, it was shown that standardized extracts of Turkish poplar-type propolis, containing mainly 3-O-methylquercetin, chrysin, caffeic acid, CAPE, galangin, and pinocembrin, induced cell cycle arrest and resulted in accumulation in the G0/G1 phase of cancer cells [ 77 ]. The effectiveness of EEPs against various cancer cells, for example, leukemia, osteosarcoma, glioblastoma, breast adenocarcinoma, melanoma, prostate cancer, has been demonstrated in vitro [ 64 , 78 , 79 ], indicating the possibility of using this preparation in the treatment of neoplastic diseases.…”

Section: Resultsmentioning

confidence: 99%

The Study of Chemical Profile and Antioxidant Properties of Poplar-Type Polish Propolis Considering Local Flora Diversity in Relation to Antibacterial and Anticancer Activities in Human Breast Cancer Cells

et al. 2022

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Nine samples of ethanolic extracts of poplar-type propolis (EEP) originated from South-Eastern Poland were analyzed in terms of the diversity of the flora around the apiary. The mineral composition, antioxidant properties, polyphenolic profile (HPTLC), and main polyphenolic constituents (HPLC-DAD) were determined. Only minor differences in chemical composition and antioxidant capacity between tested EEPs were found regardless of their botanical origin. However, the biological activity of the EEPs was more diversified. The tested EEPs showed stronger antibacterial activity against Gram-negative bacteria (Escherichia coli) compared to Gram-positive bacteria (Staphylococcus aureus and Staphylococcus epidermidis). Staphylococci biofilm inhibition occurred as a result of exposure to the action of four out of nine EEPs (P1–P4). Due to the various compositions of individual EEPs, a different MCF-7 cellular response was observed according to inhibition of cells migration and proliferation. Almost every sample inhibited the migration of breast cancer cells at a low concentration (0.04 µg/mL) of propolis. Even at the lowest concentration (0.02 µg/mL), each EEP inhibited the proliferation of MCF-7 cells, however, the level of inhibition varied between samples.

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“…The preparation of S. xanthocarpum loaded niosomes (SXE-Ns) was done by significant modifications ( Cetin et al, 2022 ). For the preparation of niosomes, materials used were Span 20, cholesterol and S. xanthocarpum extract.…”

Section: Methodsmentioning

confidence: 99%

In-vitro and ex-vivo antidiabetic, and antioxidant activities of Box-Behnken design optimized Solanum xanthocarpum extract loaded niosomes

Tyagi,

Waheed,

Kumar

et al. 2023

Saudi Pharmaceutical Journal

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Preparation of Ethanol Extract of Propolis Loaded Niosome Formulation and Evaluation of Effects on Different Cancer Cell Lines

Cited by 12 publications

References 62 publications

Nano-Delivery System of Ethanolic Extract of Propolis Targeting Mycobacterium tuberculosis via Aptamer-Modified-Niosomes

Nano-Delivery System of Ethanolic Extract of Propolis Targeting Mycobacterium tuberculosis via Aptamer-Modified-Niosomes

The Study of Chemical Profile and Antioxidant Properties of Poplar-Type Polish Propolis Considering Local Flora Diversity in Relation to Antibacterial and Anticancer Activities in Human Breast Cancer Cells

In-vitro and ex-vivo antidiabetic, and antioxidant activities of Box-Behnken design optimized Solanum xanthocarpum extract loaded niosomes

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