Preparation of extracts from Juniper leaves for electrophoresis

Kelley, Walter A.; Adams, Robert P.

doi:10.1016/0031-9422(77)80004-6

Cited by 44 publications

(13 citation statements)

References 17 publications

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“…Therefore, our standard extraction buffer seems to be sufficiently effective in stabilizing proteins. Upon addition of ascorbate we could not observe the improved banding pattern reported by Kelley and Adams (1977) studying electrophoretic patterns of Juniperus scopulorum extracts. On the contrary, ascorbate even inhibited G6PDH and 6PGDH.…”

Section: Discussioncontrasting

confidence: 65%

“…The effects of ascorbate, borate, PVPP, and thiols (here, 2-mercaptoethanol) as protective substances in plant tissue extraction are discussed in detail by King (1971), Loomis (1974) and Kelley and Adams (1977). These authors also summarize the effectiveness of various protective agents in relation to pH.…”

Section: Discussionmentioning

confidence: 96%

“…Studies on enzymes from vegetative tissues of conifers are, however, hampered by the presence of large amounts of secondary products (phenolic substances, tannins) and of phenolase activity (Haissig and Schipper 1975;Kelley and Adams 1977;Pitel and Cheliak 1985). Although the presence of phenolase in conifers has been questioned (Sherman et al 1991), there is an enzyme-catalyzed browning of extracts from conifer tissues and concomitant loss of extractable enzyme activities (Halssig and Schipper 1975).…”

Section: Introductionmentioning

confidence: 97%

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Kinetic and electrophoretic characterization of NADP dependent dehydrogenases from root tissues of Norway spruce [Picea abies (L.) Karst.] employing a rapid one-step extraction procedure

Guttenberger

Schaeffer

Hampp

1994

Trees

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Summary. The NADPH generating enzymes glucose-6-phosphate dehydrogenase (EC 1.1.1.49), 6-phosphogluconate dehydrogenase (EC 1.1.1.44), and isocitrate-dehydrogenase (NADP dependent; EC 1.1.1.42) have been characterized in spruce [Picea abies (L.) Karst.] roots. Interference from inherent phenolic compounds was minimized by complexation with borate and insoluble polyvinylpyrrolidone in the presence of 2-mercaptoethanol and NADP. A further addition of protective substances had no (bovine serum albumin) or even an inhibitory effect (ascorbate) on the enzyme activities. The enzymes were shown to be strictly NADP specific. The optimal pH values and the apparent Michaelis constants from spruce roots are in good agreement with data from different photosynthetic organisms and gametophytic tissues of conifers. Native electrophoresis and subsequent activity staining showed the same banding patterns for enzymes both from root and needle tissues. In addition, the applicability of a highly sensitive dot-blot assay for the accurate quantification of the extracted protein is shown.

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Section: Discussioncontrasting

confidence: 65%

Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 97%

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Kinetic and electrophoretic characterization of NADP dependent dehydrogenases from root tissues of Norway spruce [Picea abies (L.) Karst.] employing a rapid one-step extraction procedure

Guttenberger

Schaeffer

Hampp

1994

Trees

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“…Horton and Vitt (1976) and Crum and Anderson (1981) Plants were refrigerated in a moistened state for 2-3 days prior to enzyme extraction. Several extraction buffers were tried in a preliminary study, including those described by Kelley and Adams (1977) for sequestering phenolic compounds released by Juniperus leaves, and by Soltis et a!. (1983) for extracting enzymes from gametophytes of ferns and fern allies.…”

Section: Introductionmentioning

confidence: 99%

Electrophoretic evidence of reproductive isolation between two varieties of the moss, Climacium americanum

1987

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“…As mangrove plants contain large amount of phenolics, 7% polyvinyl pyrollidine (w/v) was included in the extraction medium to prevent the browning that occurs when large amounts of phenolics form complexes with enzymes (Kelley and Adams 1977). The homogenate was placed on ice for about 1 h and centrifuged at 26 000g for 15 min.…”

Section: Polyamine Determinationmentioning

confidence: 99%

Changes in polyamine contents and arginine decarboxylase activity associated with elongation growth of hypocotyls inRhizophora apiculataBl.

Swamy¹,

Usha²,

Kiranmayee³

et al. 2004

Can. J. Bot.

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Changes in polyamine (putrescine, spermidine, spermine) contents and arginine decarboxylase activity in three different regions of developing hypocotyls of Rhizophora apiculata Bl. were analyzed. Among the three polyamines, spermidine was present in higher quantities than either putrescine or spermine. Spermine occurred in the lowest quantities. Spermidine declined rapidly with the age of the hypocotyl. In contrast, a marked increase in putrescine was observed with the age of the hypocotyl. Arginine decarboxylase activity increased during the various developmental stages of hypocotyl growth, particularly in the final stage. The results suggest that putrescine plays a major role in the growth of the hypocotyl as evidenced by simultaneous increase in the content of putrescine and arginine decarboxylase activity. Résumé :Les auteurs ont analysé les changements qui surviennent au niveau des polyamines (la putrescine, la spermidine et la spermine) et de l'activité de la décarboxylase de l'arginine, dans trois régions distinctes de l'hypocotyle en développement chez le Rhizophora apiculata Bl. Parmi les trois polyamines, la spermidine est présente en plus grandes quantités, lorsqu'on la compare à la putrescine ou à la spermine. On retrouve la spermine en plus faibles quantités. La spermidine diminue plus rapidement avec l'âge de l'hypocotyl. Au contraire, on observe une augmentation marquée de la putrescine avec l'âge de l'hypocotyl. L'activité de la décarboxylase de l'arginine augmente au cours des divers stades du développement de la croissance de l'hypocotyle, plus particulièrement au stade final. Les résultats suggèrent que la putrescine joue un rôle majeur dans la croissance de l'hypocotyle, comme le montre l'augmentation simultanée de la teneur en putrescine et de l'activité de la décarboxylase de l'arginine.

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Preparation of extracts from Juniper leaves for electrophoresis

Cited by 44 publications

References 17 publications

Kinetic and electrophoretic characterization of NADP dependent dehydrogenases from root tissues of Norway spruce [Picea abies (L.) Karst.] employing a rapid one-step extraction procedure

Kinetic and electrophoretic characterization of NADP dependent dehydrogenases from root tissues of Norway spruce [Picea abies (L.) Karst.] employing a rapid one-step extraction procedure

Electrophoretic evidence of reproductive isolation between two varieties of the moss, Climacium americanum

Changes in polyamine contents and arginine decarboxylase activity associated with elongation growth of hypocotyls inRhizophora apiculataBl.

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