Preparation of Flavine Adenine Dinucleotide by Paper Electrophoresis.

Mechanism of Enzyme Action—an Approach Through the Study of Slow Reactions

“…These spectroscopic observations may indicate that the formation of a ternary complex is brought about by the combination of the apoenzyme, the isoalloxazine moiety of FAD, and benzoate. This is supported by still other evidence: the yellow fluorescence, which is specific for the isoalloxazine nucleus of FAD, is somewhat quenched by combining with the apoenzyme as reported by Walaas et al (26) and by Yagi et al (51), but the quenching is further pronounced by the addition of benzoate.…”

Inhibition of fumarylacetoacetate fumarylhydrolase by monovalent anions

“…Series of anions arranged in order of ability to modify some observable function and similar in sequence to the series of anions found by Hofmeister are usually referred to as the Hofmeister or Lyotropic series (Alexander, 1937). Comparable series have been obtained on the basis of the effects of salts on such diverse phenomena as the surface tension, compressibility, and viscosity of water (Traube, 1910;Bingham, 1941;Randles, 1957), the electrophoretic mobility of proteins (Longsworth and Jacobsen, 1949), and the molecular weight (Wolff, 1962), urea sensitivity (Rajagopalan et al., 1961), and activity (Massey, 1953; Walaas and Walaas, 1956;Richards and Rutter, 1961;Fridovich, 1963) of enzymes. The general order of the Hofmeister series for the anions is (Mysels, 1959): F-, I03-, H2PG4-, Br03~, Cl-, C103-, Br-, N03-, C104-, I-, and SCN-.…”

On the Interpretation of the Absorption Spectra of Flavoproteins with Special Reference to D-Amino Acid Oxidase*

“…By fluorescence-intensity studies, Walaas and Walaas (1956) determined a pK of 10.5. Figure 10 shows the effect on the visible spectrum of FAD of change in [H+] in the range pH 7-12.…”