Preparation of Folate‐Conjugated Pluronic F127/Chitosan Core‐Shell Nanoparticles Encapsulating Doxorubicin for Breast Cancer Treatment

Abstract

A targeting drug delivery system using folate-conjugated pluronic F127/chitosan core-shell nanoparticles was developed to deliver doxorubicin (DOX) to the target cancer cells. First, DOX was encapsulated in pluronic F127 micelle cores in the presence of sodium dodecyl sulfate (SDS) by a self-assembly method. To form a shell, a layer of either chitosan or folate-conjugated chitosan was deposited onto the pluronic micelles. The encapsulation efficiency was approximately58.1±4.7%. The average size of the core-shell nanoparticles was37.4±2.0 nm, while the zeta potential was12.9±2.3 mV, indicating the presence of a shell layer and more stable particles. In anin vitroDOX release study, an initial burst release, followed by a sustained release, was observed within 24 hours. In addition, the core-shell nanoparticles showed greater cytotoxicity towards MCF-7 cells than free DOX, suggesting a better therapeutic efficacy in treating cancer.

Co-delivery of IL17RB siRNA and doxorubicin by chitosan-based nanoparticles for enhanced anticancer efficacy in breast cancer cells

“…So, this naturally abundant polysaccharide has taken on an important role in the novel drug delivery systems (DDS) with the purpose of carrying out targeted delivery [37,38].…”

Selection of stabilizing agents to provide effective penetration of gold nanoparticles into cells

“…For instance, Tahara et al [40] found that A549 cells internalize chitosan-modified poly(DL-lactic-co-glycolic acid) nanospheres much better compared to the unmodified ones. Manaspon et al [12] showed that cellular uptake of chitosan nanoparticles, which have no targeting moiety on the surface, was probably mediated by nonspecific adsorptive endocytosis. The positive charge of chitosan nanoparticles can be easily attracted by negatively charged cell membranes, allowing the particles to be taken into the cells [12].…”

“…Manaspon et al [12] showed that cellular uptake of chitosan nanoparticles, which have no targeting moiety on the surface, was probably mediated by nonspecific adsorptive endocytosis. The positive charge of chitosan nanoparticles can be easily attracted by negatively charged cell membranes, allowing the particles to be taken into the cells [12]. However, in the present study it was found that the number of chitosan-stabilized GNPs which penetrate into the cells is not great.…”

“…Pluronic ® F-127 (PF127) is a triblock copolymer consisting of a central hydrophobic block flanked by two hydrophilic blocks and has molecular weight of about 13 kDa. It spontaneously forms micelles with a diameter of 30-50 nm in dilute solutions with hydrophobic central nucleus and hydrophilic residues directed toward the external surface of the molecule [11,12]. Due to this property, PF127 can be used for delivery of different pharmaceuticals into the body, including anticancer drugs [13].…”

Folic Acid Decorated Chitosan Nanoparticles and its Derivatives for the Delivery of Drugs and Genes to Cancer Cells

“…Cell viability was not disturbed by nanoparticles at concentrations lesser than 1 mg/ml (ref. 43). Folate-conjugated chitosan was loaded with other polymers or inorganic materials like graphene oxide (GO), carbon nanotube (CNT) with the anticancer drug doxorubicin (DOX) through - staking.…”