Preparation of freeze‐dried bioluminescent bacteria and their application in the detection of acute toxicity of bisphenol A and heavy metals

Hu, Shulin; Liao, Anqi; Weng, Yetian; Liang, Shuli; Lin, Ying

doi:10.1002/fsn3.2800

Cited by 12 publications

(12 citation statements)

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“…qinghaiensis Q67. The freeze–dried Q67, used for the detection of the acute toxicity alone and in the mixture, was prepared according to a previous study [ 48 ]. The contaminants used in this study were bisphenol A (BPA), K 2 Cr 2 O 7 , Cd(NO 3 ) 2 , ZnSO 4 ·7H 2 O, Pb(NO 3 ) 2 , arsenic (As), mercury (Hg), and nickel (Ni).…”

Section: Methodsmentioning

confidence: 99%

Acute and Chronic Toxicity of Binary Mixtures of Bisphenol A and Heavy Metals

Liao

Zheng

et al. 2022

Toxics

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Bisphenol A (BPA) and heavy metals are widespread contaminants in the environment. However, the combined toxicities of these contaminants are still unknown. In this study, the bioluminescent bacteria Vibrio qinghaiensis Q67 was used to detect the single and combined toxicities of BPA and heavy metals, then the joint effects of these contaminants were evaluated. The results show that chronic toxicities of chromium (Cr), cadmium (Cd), lead (Pb), arsenic (As), mercury (Hg), nickel (Ni), and BPA were time–dependent; in fact, the acute toxicities of these contaminants were stronger than the chronic toxicities. Furthermore, the combined toxicities of BPA and heavy metals displayed BPA + Hg > BPA + Cr > BPA + As > BPA + Ni > BPA + Pb > BPA + Cd in the acute test and BPA + Hg > BPA + Cd > BPA + As > BPA + Cd in the chronic test, which suggested that the combined toxicity of BPA and Hg was stronger than that of other mixtures in acute as well as chronic tests. Additionally, both CA and IA models underestimated the toxicities of mixtures at low concentrations but overestimated them at high concentrations, which indicates that CA and IA models were not suitable to predict the toxicities of mixtures of BPA and heavy metals. Moreover, the joint effects of BPA and heavy metals mainly showed antagonism and additive in the context of acute exposure but synergism and additive in the context of chronic exposure. Indeed, the difference in the joint effects on acute and chronic exposure can be explained by the possibility that mixtures inhibited cell growth and luminescence in chronic cultivation. The chronic toxicity of the mixture should be considered if the mixture results in the inhibition of the growth of cells.

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Section: Methodsmentioning

confidence: 99%

Acute and Chronic Toxicity of Binary Mixtures of Bisphenol A and Heavy Metals

Liao

Zheng

et al. 2022

Toxics

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“…Варто зазначити, що біолюмінесценція вже використовується у світовій практиці оцінки впливу важких металів на об'єкти природного середовища (це в основному водні екосистеми) (Ranjan et al, 2012;Zeb et al, 2017;Adnan et al, 2021;Yang et al, 2022).…”

Section: обговоренняunclassified

“…Окрім цього, дослідниками встановлено різну чутливість штамів фотобактерій щодо важких металів. Так, через 30 хв 50 % зниження інтенсивності світіння штаму Vibrio qinghaiensis Q67 в досліджуваній воді відбувалося за концентрації Арсену на рівні 4,674 ± 0,013 мг/л, Photobacterium phosphoreum T3 і Photobacterium phosphoreum 502 на рівні 0,229 ± 0,008 і 0,252 ± 0,002 мг/л відповідно та штам Vibrio fischeri -на рівні 3,851 ± 0,017 мг/л (Yang et al, 2022). У інших дослідженнях (He et al, 2015) 50 % зниження інтенсивності світіння штам Photobacterium phosphoreum T3 набував через 15 хв за концентрацій Арсену залежно від рН від 0,895 до 4,396 мг/л.…”

Section: обговоренняunclassified

“…рhosphoreum на обраних для експерименту рівнях не було досягнуто, як і у випадку з Арсеном. За даними (Yang et al, 2022) У нашому досліді концентрації Плюмбуму в кінцевому досліджуваному екстракті складали 0,25; 0,50; 2,50; 12,50 і 25,0 мг/л (відповідно рівням у кормі -0,50; 1,00; 5,0; 25,0 і 50,0 мг/кг), при цьому максимальний відсоток пригнічення інтенсивності світіння був на рівні 40,8 %, тобто 50 % зниження інтенсивності світіння Ph. рhosphoreum на обраних для експерименту рівнях не було досягнуто, як і у випадку з двома вищевказаними важкими металами.…”

Section: обговоренняunclassified

“…рhosphoreum на обраних для експерименту рівнях не було досягнуто, як і у випадку з двома вищевказаними важкими металами. За даними (Yang et al, 2022) За умов внесення в корм Меркурію 0,01; 0,02; 0,1; 0,5 і 1,0 мг/кг корму концентрації його в кінцевому досліджуваному екстракті складали 0,005; 0,01; 0,05; 0,25 і 0,50 мг/л відповідно. При цьому максимальний відсоток пригнічення інтенсивності світіння був на рівні 21,2 %, тобто 50 % зниження інтенсивності світіння Ph.…”

Section: обговоренняunclassified

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Toxicological evaluation of feeds with different levels of heavy metals using luminescent microorganisms Photobacterium рhosphoreum

Курбацька

Orobchenko

2022

SMLNUVMB

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Assessment of the toxicity of pollutants is an integral part of the quality and safety control of the animal feed. Today, when determining the toxicity of a particular substance, alternative methods are increasingly being used, which involve using cell cultures, protozoa, and photobacteria in a toxicological experiment. The bioluminescence effect of bacteria makes it possible to use them as a substitute for laboratory animals or as an additional test to determine the effect of toxicants, which significantly reduces the cost of work due to the simplicity and speed of research, high sensitivity, and reproducibility. This work aimed to conduct a toxicological assessment of feeds with different levels of heavy metals using the luminescent microorganisms Photobacterium phosphoreum. Under the study of heavy metals, corn grits, which did not possess toxic properties, were used as a “matrix.” Heavy metals were used in the form of State standard samples: Arsenic, Cadmium, Lead, Mercury, Copper, and Zinc. Before adding heavy metals to the feed, the “matrix” was previously examined for their content (background). Toxicants were added to the “matrix” in different concentrations, taking into account the “background” indicators (5 series each), which were prepared by diluting in distilled water, depending on the maximum residue limits (MRL). As a result of the work, the possibility of using luminescent microorganisms Photobacterium phosphoreum (strain IMV B-7071; Sq3) was established for rapid toxicological evaluation of feeds with different levels of heavy metals based on the decrease in the intensity of the glow. However, if for Cd, Hg, Zn, Cu under the conditions of the study of feed with the content of heavy metals at the MRL (0.4; 0.1; 25.0 and 120.0 mg/kg, respectively), the feed was characterized as “non-toxic,” then for Pb and As according to the MRL (5.0 and 0.5 mg/kg, respectively), the feed was characterized as “toxic,” which indicates the need for further studies on the toxicological characteristics of heavy metals in the body of laboratory and productive animals, possibly with a further revision (downward) of the MRL of the relevant pollutants in feed in Ukraine.

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Implication of storage conditions on luminescence response from Photobacterium phosphoreum in free and immobilized form

et al. 2023

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Bioluminescent bacteria in the form of a cell suspension for on-site hazard analysis are not suitable as in vivo luminescence in free cells fluctuates and may lead to erroneous results. Furthermore, the culture broth cannot be stored for long durations to continue sensing analytes as the luminescence ceases over time. Factors that affect luminescence response include growth dynamism, and ambient environmental conditions. The present study investigated the effect of storage conditions such as temperature (25 ± 2 C, room temperature; 4 C; and À20 C) and ambient aqueous environment (M1: sucrose, 1.02 M; M2, bioluminescent media [tryptone, 10 g L À1 ; NaCl, 28.5 g L À1 ; MgCl 2 .7H 2 O, 4.5 g L À1 ; CaCl 2 , 0.5 g L À1 ; KCl 0.5 g L À1 ; yeast extract, 1 g L À1 ; H 2 O, 1 L]; M3, bioluminescent media and 95% glycerol, 1:1 ratio) on the luminescence emission from the calcium alginate-immobilized Photobacterium phosphoreum (S b ) against the cells in free suspension for an extended period. The results indicated that both the parameters that were undertaken markedly affected the luminescence. In the study, S b showed an enhanced luminescence emission than the control up to 18.5-fold and for a prolonged period which can be efficiently utilized for rapid biosensing of hazardous materials.

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Preparation of freeze‐dried bioluminescent bacteria and their application in the detection of acute toxicity of bisphenol A and heavy metals

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 12 publications

References 69 publications

Acute and Chronic Toxicity of Binary Mixtures of Bisphenol A and Heavy Metals

Acute and Chronic Toxicity of Binary Mixtures of Bisphenol A and Heavy Metals

Toxicological evaluation of feeds with different levels of heavy metals using luminescent microorganisms Photobacterium рhosphoreum

Implication of storage conditions on luminescence response from Photobacterium phosphoreum in free and immobilized form

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