Preparation of Fritless Packed Silica Columns for Capillary Electrochromatography

Fujimoto, Chuzo

doi:10.1002/(sici)1521-4168(20000101)23:1<89::aid-jhrc89>3.0.co;2-5

Cited by 71 publications

(21 citation statements)

References 25 publications

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“…The rather low plate count for the pressuredriven separations was attributed to the A-term contributions of the van Deemter equation; thereby, the monolithic system seems more appropriate for CEC than for HPLC. Honda et al [100] and Fujimoto [95,170] have also followed a similar procedure to fabricate monolithic columns for CEC. The sol-gel-based monolithic columns showed higher permeability than columns packed with particles of about 3 mm.…”

Section: Silica-based Monolithsmentioning

confidence: 99%

Recent progress in capillary electrochromatography

et al. 2000

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Capillary electrochromatography (CEC) continues to captivate many separation scientists. A remarkable activity is apparent from the numerous publications in the literature using CEC. A review of the most recent progress in CEC is presented herein, covering an extensive fraction of the literature on CEC published from the year 1997 until the beginning of 2000. Most of the recent developments have concentrated on column technology.

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Section: Silica-based Monolithsmentioning

confidence: 99%

Recent progress in capillary electrochromatography

et al. 2000

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“…The monolithic silica matrix was prepared according to [16,17] with modifications. Briefly, 0.5 mL of 0.01 M acetic acid, 54 mg PEG, and 0.2 mL TMOS were mixed in a 1.5-ml-vial bottle.…”

Section: Preparation Of the Silica-based Monolithic Column Matrix By mentioning

confidence: 99%

ChemicallyL-prolinamide-modified monolithic silica column for enantiomeric separation of dansyl amino acids and hydroxy acids by capillary electrochromatography and μ-high performance liquid chromatography

2001

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A silica-based chiral monolithic column prepared by sol-gel process and chemical modification of chiral selector was used for enantioseparation of dansyl amino acids and hydroxy acids by capillary electrochromatography (CEC) and mu-high-performance liquid chromatography (mu-HPLC). L-Prolinamide was modified as a chiral selector. The chiral stationary phase (CSP), the chiral complex of Cu(II) with L-prolinamide, provides an anodic electroosmotic flow (EOF) in CEC. The EOF was found to be dependent on applied electric field strength, the pH, and the composition of mobile phases. Scanning electron micrograph showed that monolithic columns have the morphology of continuous skeleton and large through-pore. D-Enantiomers migrated before L-enantiomers except for dansyl-(Dns)-DL-Ser. The separation efficiencies of up to 17600 (D) and 13,200 plates m(-1) (L) were achieved for the separation of DL-indole-3-lactic acid.

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“…Frits break easily and are the sites of bubble formation, which disrupts the conduction of the electrical current thus causing the complete stop of the CEC process. To overcome these problems arising from the presence of retaining frits, fritless columns having monolithic stationary phases based on either porous polymers [1,[11][12][13] or porous silica-based monoliths [14][15][16][17] have been introduced. In situ polymerization of polymer-based monoliths represents several advantages over silica-based monoliths including the ease with which they can be made and the much larger available chemistries which can be performed to produce tailor-made columns in terms of the monolith porosity and the nature of its retentive ligand.…”

Section: Introductionmentioning

confidence: 99%

Capillary electrochromatography with monolithic stationary phases: 1. Preparation of sulfonated stearyl acrylate monoliths and their electrochromatographic characterization with neutral and charged solutes

2002

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A novel monolithic stationary phase having long alkyl chain ligands (C17) was introduced and evaluated in capillary electrochromatography (CEC) of small neutral and charged species. The monolithic stationary phase was prepared by the in situ copolymerization of pentaerythritol diacrylate monostearate (PEDAS) and 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) in a ternary porogenic solvent consisting of cyclohexanol/ethylene-glycol/water. While AMPS was meant to support the electroosmotic flow (EOF) necessary for transporting the mobile phase through the monolithic capillary, the PEDAS was introduced to provide the nonpolar sites for chromatographic retention. Monolithic columns at various EOF velocities were readily prepared by conveniently adjusting the amount of AMPS in the polymerization solution as well as the composition of the porogenic solvent. The monolithic stationary phases thus obtained exhibited reversed-phase chromatography behavior toward neutral solutes and yielded a relatively strong EOF. For charged solutes (e.g., dansyl amino acids), nonpolar as well as electrostatic interaction/repulsion with the monoliths were observed in addition to electrophoretic migration. Therefore, for charged solutes, selectivity and migration can be readily manipulated by changing various parameters including the nature of the monolith and the composition of the mobile phase (e.g., pH, ionic strength and organic modifier). Ultrafast separation on the time scale of seconds of 17 different charged and neutral pesticides and metabolites were performed using short capillary columns of 8.5 cm x 100 microm ID.

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Preparation of Fritless Packed Silica Columns for Capillary Electrochromatography

Cited by 71 publications

References 25 publications

Recent progress in capillary electrochromatography

Recent progress in capillary electrochromatography

ChemicallyL-prolinamide-modified monolithic silica column for enantiomeric separation of dansyl amino acids and hydroxy acids by capillary electrochromatography and μ-high performance liquid chromatography

Capillary electrochromatography with monolithic stationary phases: 1. Preparation of sulfonated stearyl acrylate monoliths and their electrochromatographic characterization with neutral and charged solutes

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