Preparation of Frozen Sections

Davis, Donald R.; Pellowski, Donna M.; Hanke, C. William

doi:10.1111/j.1524-4725.2004.30506.x

Cited by 27 publications

(30 citation statements)

References 48 publications

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“…A variety of devices have been described to help conform tissue specimens to a shape that will allow sectioning for Mohs surgery: heat extractor, glass slide, piece of x-ray film, Miami Special, Cryomold, Cryocup, and CryoHist. 6 The initial cost, cost of consumables, ease of use, speed and efficiency, space requirements, durability, and need for an additional source of cryogen should be considered when comparing these devices.…”

Section: Discussionmentioning

confidence: 99%

Face-Down Cryoembedding of Tissue Specimens for Mohs Micrographic Surgery Using Stainless Steel Embedding Wells

Pike

Tusa

Lenis

et al. 2009

Dermatologic Surgery

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Section: Discussionmentioning

confidence: 99%

Face-Down Cryoembedding of Tissue Specimens for Mohs Micrographic Surgery Using Stainless Steel Embedding Wells

Pike

Tusa

Lenis

et al. 2009

Dermatologic Surgery

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“…A full review of benefits and drawbacks of the different techniques is included in Table 1. [1][2][3][4] We hypothesized that high histopathologic specimen quality could be maintained with non-beveled Mohs layers. This study is limited in its retrospective design, restricted to a single institution, and the fact the slides were scored by the operating surgeon only.…”

Section: = Non-beveled Incision; Surgeon 2 = Beveled Incision) Using mentioning

confidence: 99%

A single‐institution retrospective evaluation of Mohs incision angles and histopathologic specimen quality

Olbricht

Tiger

2019

Int J Dermatology

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Background Traditionally, Mohs layers are excised using a beveled incision to facilitate tissue flattening for tangential sectioning. Some surgeons perform non‐beveled incisions; however, these specimens may be harder for the histotechnician to process. Limited data exist comparing slide quality between these techniques. Methods Retrospective review of cases performed by two Mohs surgeons (surgeon 1 = non‐beveled incision; surgeon 2 = beveled incision) using different incision angles between June 2014 and December 2016. Daily histopathologic slide quality assessment scores (maximum score = 5, minimum score = 1) of the day's first case were compared. Results About 536 slides (surgeon 1 = 277, surgeon 2 = 259) were evaluated from 2,825 cases. Mean quality assessment scores were similar between surgeons (4.89 and 4.86; P = 0.31) with missing or folded epidermis being the most commonly reported issue for both surgeons. Conclusion Similar slide quality can be achieved via both beveled and non‐beveled Mohs cutting angles. While more relaxing incisions may be necessary to optimize tissue flattening with non‐beveled incisions, there is no associated increased loss of epidermal margins. The potential benefits of non‐beveled incisions, such as minimizing tangentially cut adnexal structures and creating vertical wound edges optimized for repair, may offer an alternative technique with positive clinical implications.

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“…The purpose of this article is to compare the innovative dry ice technique 12 with two standard methods currently used for tissue processing in Mohs surgery, the Heat Sink method 5,13 and the Miami Special 7 , using an animal model.…”

Section: Many Innovative Techniques Have Been Developed To Improvementioning

confidence: 99%

“…One of the most popular techniques used to flatten and freeze specimens is the heat extractor method, used for flattening and freezing the tissues inside the cryostat 12,13 . However, the warm weather and high temperatures, increase the thermal exchange between the cryostat chamber and the environment, and it can slow down the process, especially when many samples need to be processed 7,12 .…”

Section: Many Innovative Techniques Have Been Developed To Improvementioning

confidence: 99%

Validation of a new technique to freezing and embedding tissue in Mohs surgery, using an animal model

Sanchez

Filho²,

Russo

2016

Acta Cir. Bras.

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PURPOSE:To validate the innovative Dry Ice method, comparing it with two standard methods currently used for tissue processing in Mohs surgery, the Heat Sink method and the Miami Special. METHODS:Forty eight samples of pigs kin with the standard beveled Mohs technique were used, and randomly allocated into six groups. Each group was processed with one of the 3 methods and evaluated for: The freezing time, the depth required to cut into the block to obtain a complete section, and the quality of histological slides analyzed with a image software. The statistical analysis was performed with the software SAS ® System. The inferential analysis was made by one-way ANOVA. RESULTS:The Miami Special showed a processing time significantly shorter than Dry Ice method and Heat Sink method. There was no significant difference in the depth required to cut into the blocks, and area of surgical margins visualized. CONCLUSION:The Dry Ice method was as efficient as the other two methods currently used in Mohs surgery, considering the individual advantages and disadvantages of each method.

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Preparation of Frozen Sections

Abstract: We dissect each step of micrographic cancer removal, report on the standard and preferred methods of tissue preparation, and summarize the progress achieved in the past decade.

Cited by 27 publications

References 48 publications

Face-Down Cryoembedding of Tissue Specimens for Mohs Micrographic Surgery Using Stainless Steel Embedding Wells

Face-Down Cryoembedding of Tissue Specimens for Mohs Micrographic Surgery Using Stainless Steel Embedding Wells

A single‐institution retrospective evaluation of Mohs incision angles and histopathologic specimen quality

Validation of a new technique to freezing and embedding tissue in Mohs surgery, using an animal model

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