Preparation of Frozen Sections

Peripheral Frozen Sections

“…Although it requires different grossing techniques than classically taught to skin cancer surgeons, 90°sections have the advantages of smaller defects and tissue sparing, are a natural choice after debulking, and will be second nature to pathologists and HTs. 17 Most dermatologists put ink marks on the patient's skin to orient the removed specimen to the patient.…”

“…Literally scores of techniques and machines have been created in the past decades to produce complete peripheral margins. 17 Most of the mounting techniques apply force to flatten the outside margin, something largely accomplished by relaxing incisions. Forceps placement followed by a heat extractor compression is the most used and most useful.…”

“…20 Various embedding media are available and can even be colored to help separate the multiple cases that are simultaneously processed. 17 Tissue dehydration during grossing will solve most embedding problems.…”

“…17 Most slides are 1 mm thick, are made of glass, and are designed to have a refractive index of 1.5, the same as fixed skin protein. Silicate glass does not have a uniform or adherent surface.…”

“…Cartilage may require adhesives for adequate attachment. 17 Moisture and residue on the slide are the commonest causes of section wash-off.…”