Preparation of Functional Human Hepatocytes Ex Vivo

Okumura, Ayumu; Tanimizu, Naoki

doi:10.1007/978-1-0716-2557-6_20

Cited by 1 publication

(2 citation statements)

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“…Indeed, in vitro models of human liver preparations seem to be the most lucrative models with maximum feasibility. Cellular and subcellular systems are included in these models, and they can equally contain HepG2 and HuH7 hepatic cell lines (developed from liver tumors and preserved hepatocytes) [ 74 , 75 , 76 ]. The primary functional cells of the liver, the hepatocytes, have historically been challenging to culture ex vivo.…”

Section: Relevant Matricesmentioning

confidence: 99%

“…According to Le Daré et al, a higher quetiapine metabolism was observed in differentiated HepaRG cells (50% of quetiapine metabolized) compared to pHH (25% of quetiapine metabolized) [71][72][73]. This helps meet the desired feature of human cell models, stably expressing the functional properties of the in vivo cells they are derived from in order to predict the toxicity of chemicals [74]. Indeed, in vitro models of human liver preparations seem to be the most lucrative models with maximum feasibility.…”

Section: Liver Tissue Modelingmentioning

confidence: 99%

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Quetiapine-Related Deaths: In Search of a Surrogate Endpoint

Šoša

2024

Toxics

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Quetiapine is a second-generation antipsychotic drug available for two and half decades. Due to increased misuse, prescription outside the approved indications, and availability on the black market, it is being encountered in medicolegal autopsies more frequently. For instance, it has been linked to increased mortality rates, most likely due to its adverse effects on the cardiovascular system. Its pharmacokinetic features and significant postmortem redistribution challenge traditional sampling in forensic toxicology. Therefore, a systematic literature review was performed, inclusive of PubMed, the Web of Science—core collection, and the Scopus databases; articles were screened for the terms “quetiapine”, “death”, and “autopsy” to reevaluate each matrix used as a surrogate endpoint in the forensic toxicology of quetiapine-related deaths. Ultimately, this review considers the results of five studies that were well presented (more than two matrices, data available for all analyses, for instance). The highest quetiapine concentrations were usually measured in the liver tissue. As interpreted by their authors, the results of the considered studies showed a strong correlation between some matrices, but, unfortunately, the studies presented models with poor goodness of fit. The distribution of quetiapine in distinct body compartments/tissues showed no statistically significant relationship with the length of the postmortem interval. Furthermore, this study did not confirm the anecdotal correlation of peripheral blood concentrations with skeletal muscle concentrations. Otherwise, there was no consistency regarding selecting an endpoint for analysis.

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Section: Relevant Matricesmentioning

confidence: 99%