Preparation of ginseng extract with enhanced levels of ginsenosides Rg1 and Rb1 using high hydrostatic pressure and polysaccharide hydrolases

Abstract

Background:Ginsenosides are the principal components responsible for the pharmacological activities of ginseng. Ginsenosides Rg1 and Rb1 are the major compounds recognized as marker substances for quality control of ginseng-based products. These major compounds can be transformed to several pharmacologically active minor ginsenosides by chemical, microbial, and enzymatic means.Materials and Methods:In the present study, a combination of polysaccharide hydrolases and high hydrostatic pressure (HHP) were used to extract ginseng saponins enriched with ginsenosides Rg1 and Rb1. Temperature, pH, time, ginseng-to-water ratio, and pressure were optimized to obtain the maximum amount of Rg1 and Rb1 in the resulting extract using commercial polysaccharide hydrolases.Results:This study showed that treatment with a combination of cellulase, amylase, and pectinase at 100 MPa pressure, pH 4.8, and 45°C for 12 h resulted in higher Rg1 and Rb1 levels in the extract.Conclusion:This study describes a cheap and ecofriendly method for preparing ginseng extract enriched with Rg1 and Rb1.SUMMARY Ginsenosides are the principal bioactive components present in ginsengGinsenosides Rg1 and Rb1 are the most abundant compounds in ginsengHigh hydrostatic pressure (HHP) and Polysaccharide hydrolases (PH) were combined to extract ginseng saponins enriched with Rg1 and Rb1Extraction conditions were optimized to obtain the maximum amount of Rg1 and Rb1Extraction with a combination of cellulase, amylase, and pectinase at 100 MPa pressure at pH 4.8, and 45°C for 12 h resulted in higher levels of Rg1 and Rb1 in the ginseng extract Abbreviations used: ATCC: American Type Culture Collection, Mpa: Mega Pascal

Herbal Medicine for the Treatment of Obesity: An Overview of Scientific Evidence from 2007 to 2017

“…Ginseng saponins and polysaccharides are the main active components of Panax ginseng C. A. Mey [60]. Ginseng saponins can be subdivided based on structure into Rb1, Rb2, Rc, Rd, Re, and Rl [36, 37]. Panax ginseng C. A. Mey can reduce body weight [61], attenuate fat accumulation [62], suppress lipid accumulation and reactive oxygen species (ROS) production [63], and improve insulin resistance [64].…”

Ginsenoside Rb1 for Myocardial Ischemia/Reperfusion Injury: Preclinical Evidence and Possible Mechanisms

“…Ginsenosides, the triterpene saponins, is one of the major components of ginseng [8]. To date, more than 30 kinds of ginsenosides have been identified [9].…”

Polysaccharides from pectinase digests of green tea enhances host immune defence through toll-like receptor 4

“…Pectinase was the cell wall may be broken down, decreasing mass transfer resistance (Barman, Sit, Badwaik, & Deka, 2014). Moreover, the reaction is mild and the products stable, and pectinase can improve extraction efficiency and shorten extraction time (Barman et al, 2014;Palaniyandi, Suh, & Yang, 2017;Zhai et al, 2018). Here, polysaccharides from pectinase digests of green tea (PPGT) was performed, using the optimal temperature and time identified from previous studies of pectinase treatment.…”