Preparation of Highly Sensitive Protein Array Using Reactive Polymer

Abstract

We have discovered a novel protein immobilization method, i.e., a "Three-Dimensional Nanostructured Protein Hydrogel" (3-D NPH), which is composed of protein-reactive polymer hybrid nanoparticles to detect protein-protein interactions. The 3-D NPH can be easily prepared by spotting a protein/reactive polymer mixture on a substrate. The resulting 3-D NPH is characterized by large amounts of immobilized proteins and a novel porous structure.The 3-D NPH technology was applied to immobilize streptavidin (SA) onto Au-coated surface for surface plasmon resonance imaging (SPRi). By using 3-D NPH method, it was possible to improve the sensitivity of protein-protein interactions drastically comparing to the conventional protein immobilization method.

Survey of the 2009 commercial optical biosensor literature

“…Full references for the 316 papers that were given the thumbs up are provided in the reference section. To make this list useful as a tool for identifying examples of the various assay formats, the citations are subgrouped by reviews/protocols dedicated to biosensor studies, kinetic, equilibrium/competition, or concentration analyses, qualitative formats (e.g., yes/no, screening, epitope mapping),…”