Preparation of Holocellulose from Nonwoody Plant Material

The Cell Walls of Higher Plants: Their Composition, Structure and Growth

“…The method of Wise et al (1946) is usually adopted; this removes the lignin by treatment of the tissue with sodium chlorite in dilute acetic acid. It has been compared quantitatively with other methods and under various conditions by Koeppen & Cohen (1953) and extended to soft tissues by Bennett (1947Bennett ( , 1948 and Jermyn si Isherwood (1956).…”

Quantitative method applicable for various biomass species to determine their chemical composition

“…The question then arises as to which components are overestimated. Bennett [21] and Angles et al [22] discovered that inorganics mainly remained in the residual fraction when organic material was dissolved and separation was made. Table 3 One is tempted to consider that ash is contaminated extensively only in high ash content samples, but even in low ash content ones like Japanese cedar, inorganic constituents still remained in holocellulose.…”