Preparation of Hydrogen Peroxide Sensitive Nanofilms by a Layer-by-Layer Technique

Yoshida, Kentaro; Ono, Tetsuya; Dairaku, Takenori; Kashiwagi, Yoshitomo; Sato, Katsuhiko

doi:10.3390/nano8110941

Cited by 4 publications

(4 citation statements)

References 48 publications

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“…If ROS are generated near the LbL films, then nonspecific cleavage of DNA is triggered. Yoshida et al reported the decomposition of H-PEI/DNA nanofilm by H2O2 [25]. Similarly, the decomposition of the (H-PEI/DNA + GOx)5 films by H2O2 resulted in a decrease in the absorption maximum derived from DNA.…”

Section: Resultsmentioning

confidence: 99%

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Decomposition of Glucose-Sensitive Layer-by-Layer Films Using Hemin, DNA, and Glucose Oxidase

et al. 2020

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Glucose-sensitive films were prepared through the layer-by-layer (LbL) deposition of hemin-modified poly(ethyleneimine) (H-PEI) solution and DNA solution (containing glucose oxidase (GOx)). H-PEI/DNA + GOx multilayer films were constructed using electrostatic interactions. The (H-PEI/DNA + GOx)5 film was then partially decomposed by hydrogen peroxide (H2O2). The mechanism for the decomposition of the LbL film was considered to involve more reactive oxygen species (ROS) that were formed by the reaction of hemin and H2O2, which then caused nonspecific DNA cleavage. In addition, GOx present in the LbL films reacts with glucose to generate hydrogen peroxide. Therefore, decomposition of the (H-PEI/DNA + GOx)5 film was observed when the thin film was immersed in a glucose solution. (H-PEI/DNA + GOx)5 films exposed to a glucose solution for periods of 24, 48 72, and 96 h indicated that the decomposition of the film increased with the time to 9.97%, 16.3%, 23.1%, and 30.5%, respectively. The rate of LbL film decomposition increased with the glucose concentration. At pH and ionic strengths close to physiological conditions, it was possible to slowly decompose the LbL film at low glucose concentrations of 1–10 mM.

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Section: Resultsmentioning

confidence: 99%

“…We have recently reported that hydrogen peroxide (H 2 O 2 ) induced the decomposition of LbL films composed of hemin-modified poly(ethyleneimine) (H-PEI) and DNA [25]. Hemin is an iron porphyrin molecule and the iron porphyrin produces more reactive oxygen species (ROS), such as hydroxy radicals (OH), by reaction with H 2 O 2 [26,27].…”

Section: Introductionmentioning

confidence: 99%

Decomposition of Glucose-Sensitive Layer-by-Layer Films Using Hemin, DNA, and Glucose Oxidase

et al. 2020

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“…The susceptibility of DNA to non-specific cleavage by reactive oxygen species (ROS) inspired the creation of H 2 O 2 -sensitive nanofilms consisting of DNA, hemin and poly(ethyleneimine) (PEI) [ 35 ]. Hemin is an iron porphyrin molecule, which served as a catalyst, producing more reactive oxygen species in its reaction with H 2 O 2 .…”

Section: Dna Based Drug Delivery Vehiclesmentioning

confidence: 99%

Sequence Does Not Matter: The Biomedical Applications of DNA-Based Coatings and Cores

Batasheva

Fakhrullin

2021

IJMS

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Biomedical applications of DNA are diverse but are usually associated with specific recognition of target nucleotide sequences or proteins and with gene delivery for therapeutic or biotechnological purposes. However, other aspects of DNA functionalities, like its nontoxicity, biodegradability, polyelectrolyte nature, stability, thermo-responsivity and charge transfer ability that are rather independent of its sequence, have recently become highly appreciated in material science and biomedicine. Whereas the latest achievements in structural DNA nanotechnology associated with DNA sequence recognition and Watson–Crick base pairing between complementary nucleotides are regularly reviewed, the recent uses of DNA as a raw material in biomedicine have not been summarized. This review paper describes the main biomedical applications of DNA that do not involve any synthesis or extraction of oligo- or polynucleotides with specified sequences. These sequence-independent applications currently include some types of drug delivery systems, biocompatible coatings, fire retardant and antimicrobial coatings and biosensors. The reinforcement of DNA properties by DNA complexation with nanoparticles is also described as a field of further research.

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“…Among ROS, H 2 O 2 boasts an extended biological lifespan and facile diffusion both within and between cells ( 27 ). Moreover, oxidative stress caused by H 2 O 2 is implicated in the pathogenesis of diverse types of disease, such as cancer, Parkinson's disease, cardiovascular disease ( 28 ). Therefore, the development of H 2 O 2 stimulus-responsive biomaterials holds promise in decreasing drug toxicity for lesions characterized by heightened oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic impacts of GNE‑477‑loaded H₂O₂ stimulus‑responsive dodecanoic acid‑phenylborate ester‑dextran polymeric micelles on osteosarcoma

Pan,

Zou,

Zhou

et al. 2024

Int J Mol Med

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Osteosarcoma (OS) is a highly malignant primary bone neoplasm that is the leading cause of cancer-associated death in young people. GNE-477 belongs to the second generation of mTOR inhibitors and possesses promising potential in the treatment of OS but dose tolerance and drug toxicity limit its development and utilization. The present study aimed to prepare a novel H 2 O 2 stimulus-responsive dodecanoic acid (DA)-phenylborate ester-dextran (DA-B-DEX) polymeric micelle delivery system for GNE-477 and evaluate its efficacy. The polymer micelles were characterized by morphology, size and critical micelle concentration. The GNE-477 loaded DA-B-DEX (GNE-477@DBD) tumor-targeting drug delivery system was established and the release of GNE-477 was measured. The cellular uptake of GNE-477@DBD by three OS cell lines (MG-63, U2OS and 143B cells) was analyzed utilizing a fluorescent tracer technique. The hydroxylated DA-B was successfully grafted onto dextran at a grafting rate of 3%, suitable for forming amphiphilic micelles. Following exposure to H 2 O 2 , the DA-B-DEX micelles ruptured and released the drug rapidly, leading to increased uptake of GNE-477@DBD by cells with sustained release of GNE-477. The in vitro experiments, including MTT assay, flow cytometry, western blotting and RT-qPCR, demonstrated that GNE-477@DBD inhibited tumor cell viability, arrested cell cycle in G1 phase, induced apoptosis and blocked the PI3K/Akt/mTOR cascade response. In vivo , through the observation of mice tumor growth and the results of H&E staining, the GNE-477@DBD group exhibited more positive therapeutic outcomes than the free drug group with almost no adverse effects on other organs. In conclusion, H 2 O 2 -responsive DA-B-DEX presents a promising delivery system for hydrophobic anti-tumor drugs for OS therapy.

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Preparation of Hydrogen Peroxide Sensitive Nanofilms by a Layer-by-Layer Technique

Cited by 4 publications

References 48 publications

Decomposition of Glucose-Sensitive Layer-by-Layer Films Using Hemin, DNA, and Glucose Oxidase

Decomposition of Glucose-Sensitive Layer-by-Layer Films Using Hemin, DNA, and Glucose Oxidase

Sequence Does Not Matter: The Biomedical Applications of DNA-Based Coatings and Cores

Therapeutic impacts of GNE‑477‑loaded H₂O₂ stimulus‑responsive dodecanoic acid‑phenylborate ester‑dextran polymeric micelles on osteosarcoma

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Preparation of Hydrogen Peroxide Sensitive Nanofilms by a Layer-by-Layer Technique

Cited by 4 publications

References 48 publications

Decomposition of Glucose-Sensitive Layer-by-Layer Films Using Hemin, DNA, and Glucose Oxidase

Decomposition of Glucose-Sensitive Layer-by-Layer Films Using Hemin, DNA, and Glucose Oxidase

Sequence Does Not Matter: The Biomedical Applications of DNA-Based Coatings and Cores

Therapeutic impacts of GNE‑477‑loaded H2O2 stimulus‑responsive dodecanoic acid‑phenylborate ester‑dextran polymeric micelles on osteosarcoma

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Therapeutic impacts of GNE‑477‑loaded H₂O₂ stimulus‑responsive dodecanoic acid‑phenylborate ester‑dextran polymeric micelles on osteosarcoma