Preparation of Immobilized Lipase on Silica Clay as a Potential Biocatalyst on Synthesis of Biodiesel

Abstract

Biodiesel offers an important alternative to fossil fuel. In this work, Eversa Transform 2.0 lipase was immobilized onto 3-aminopropyltriethoxysilane (APTES) modified silica clay (SC) by glutaraldehyde. The characteristics of the functionalized supports and the immobilized lipase were investigated by FTIR, TEM, BET, and XRD. The results show that the optimal conditions of lipase immobilization are as follows: 2% glutaraldehyde concentration, 15 mg/mL lipase concentration and incubating at 25 °C for 60 min. The immobilized lipase showed a high tolerance to temperature and pH variation in comparison to the free lipase. The immobilized lipase on SC was applied as a biocatalyst for the synthesis of biodiesel from methanol and canola oil. A biodiesel yield of 86% was obtained at a temperature of 45 °C via a three-step methanol addition. A conversion yield of 67% was maintained after reusing the immobilized lipase for five cycles. This work provides a strategy for the preparation of an efficient biocatalyst for the synthesis of biodiesel.

Preparation of immobilized pepsin for extraction of collagen from bovine hide

“…Pure SC contains a small amount of N and C, which indicates that the silica clay used contained certain organic components. 31 Compared to pure SC, the elemental contents of N, C, and H of A-SC, Glu-A-SC and Pep-Glu-A-SC increased signicantly, which should be attributed to the successful introduction of APTES. Furthermore, aer immobilization with pepsin, 0.08% of elemental S content could be observed on Pep-Glu-A-SC, illustrating that the sulfur-containing pepsin was successfully immobilized onto the support.…”

Immobilization of Eversa Lipases on Hydrophobic Supports for Ethanolysis of Sunflower Oil Solvent-Free

“…2 Effect of different molar ratios of alcohol on the sunflower oil transesterification reaction using Eversa® Transform immobilized on Sepabeads C-18 with ethanol using 10 wt% immobilized lipase using 4: 1 (E:O) molar ratio. According to the literature, several studies of enzymatic esters production, using alcohol and oil as substrates, exhibit optimal molar ratio oil:alcohol 1:3 [42][43][44][45][46][47] or 1:4 [10,11,15,[48][49][50][51].…”

Immobilization of Lipases on Modified Silica Clay for Bio-Diesel Production: The Effect of Surface Hydrophobicity on Performance

“…Glutaraldehyde-activated supports and its immobilization process with lipase were described in our previous report [26]. Briefly, 1.0 g of the modified silica clay was dispersed in 10 mL of glutaraldehyde solution at 30 • C for 2 h with gentle shaking.…”

“…In our previous study, the cheap and easily obtained silica clay showed good performance as lipase immobilization support for biodiesel production [26]. Herein, a series of silica coupling agents with various lengths of alkyl chain was coupled with silica clay for the synthesis of a series of novel alkyl-modified silica clay support to impart the support with different degrees of hydrophobicity.…”