“…This study demonstrated significant stability in in-house quality control material stored at −20 °C to −30 °C over eight months, evidenced by consistently lower variability in glucose measurements compared to other storage temperatures. These findings align with previously published data by Sweta et al [ 27 ], Eliezer et al [ 28 ], Andrea et al [ 29 ], and Berhanu et al [ 30 ], solidifying the potential of frozen storage for maintaining QC material integrity. The use of in-house pooled serum, prepared separately, has been identified as a viable alternative to conventional control materials in developing countries.…”