Preparation of inorganic–organic anion-exchange membranes and their application in plasmid DNA and RNA separation

Novel zwitterionic inorganic–organic hybrids: Synthesis of hybrid adsorbents and their applications for Cu2+ removal

“…For preparing these hybrid polymers, silane-coupling agent (or alkoxyorganosilane) is usually directly used as hybrid precursor for sol-gel process to obtain the inorganic moiety in the hybrid matrix [4]. However, these silicones can also be incorporated into the hybrid matrix via the polymerization of alkoxysilane with reactive organic monomer [5,6].…”

Purification of plasmid DNA using tangential flow filtration and tandem anion-exchange membrane chromatography

“…Separating the target pDNA from cell lysates also containing proteins, endotoxins, genomic DNA (gDNA), and abundant RNA, is thus receiving more and more attention [9].…”

“…Of the different chromatography modes available [13], anion-exchange chromatography can be considered as the real workhorse [14]. This method is most widely applied because of the fast binding between negatively charged phosphate groups in DNA backbone and positively charged groups on anion exchangers [9].…”

“…To overcome this problem, recently anion-exchange membrane chromatography has received considerable attention [9,[16][17][18]. Anion-exchange membranes have large convective pores that allow the pDNA to readily access all of the membrane's anionic binding sites, even at very-high flow rates.…”

Potential application of hydrogel-based strong anion-exchange membrane for plasmid DNA purification

“…It is noteworthy that the maximum uptake capacity per membrane volume is 6 times higher than that of a resin [5]. It is also notable that the maximum uptake capacity per cross-sectional membrane sheet area for plasmid DNA of similar linear size was much higher than that of the membranes used in previous studies [19,27]. Reversible binding of plasmid DNA was assessed by eluting the adsorbed plasmid DNA from the membrane in a batch mode.…”