Preparation of inulin-type fructooligosaccharides using fast protein liquid chromatography coupled with refractive index detection

Preparation and purification of raffinose family oligosaccharides from Rehmannia glutinosa Libosch. by fast protein liquid chromatography coupled with refractive index detection

“…Generally, Sephadex G25 and Sephadex LH20 are usually used for separation of carbohydrates with molecular weight between 150 and 5000 Da, which have been applied for separation of agaro-oligosaccharides [17] and neoagaro-oligosaccharides [18], respectively. Furthermore, the Bio-Gel P2 is effective for separation of carbohydrates with the molecular weight ranging from 150 to 1800 Da, which has been applied for separation of the fructooligosaccharides [11], galactooligosaccharides [19] and neoagaro-oligosaccharides [20]. Therefore, Sephadex G25, Sephadex LH20 and Bio-Gel P2 were selected as the candidates for separation of RFOs from R. glutinosa.…”

“…Microwave assisted extraction has been successfully applied for the extraction of carbohydrates with a number of advantages, such as short extraction time, less solvent consuming and high extraction efficiency [11][12][13]. In this case, R. glutinosa was dried at 50°C and then milled to particle size of less than 3 mm.…”

“…However, the Akta FPLC only has UV detector, which is unavailable to detect compounds without UV absorbance, such as carbohydrates. More recently, a fast protein liquid chromatography coupled with refractive index detection (FPLC-RID) method has been developed for separation and purification of oligosaccharides, which has the advantages of high automation, high separation efficiency, and chemical reagents free for monitoring the separation of carbohydrates [11]. Therefore, in this study, the RFO standards with different DP were Abbreviations: DP, degree of polymerization; FPLC-RID, fast protein liquid chromatography coupled with refractive index detection; GC-MS, gas chromatography coupled with mass spectroscopy; LC-MS/MS, liquid chromatography coupled with tandem mass spectroscopy; RFOs, Raffinose family oligosaccharides; SEC, size exclusion chromatography; TFA, trifluoroacetic acid.…”

Quality control of sweet medicines based on gas chromatography-mass spectrometry

“…TFA is still the most frequently used hydrolysis reagents. And what should be noted is that acid-sensitive sugars such as fructose also need hydrolysis in mild conditions after permethylation (94). Then the hydrolyzed free methylated monosaccharide residues are commonly reduced by NaBH 4 /NaBD 4 or NaBH 4 /NaBD 4 in NH 4 OH and converted into alditols.…”

Sugaring-out-assisted aqueous two-phase extraction of fructooligosaccharides from yacon (Smallanthus sonchifolius)

“…[14] The FOS purification methods include nanofiltration, [15] silica gel absorption, activated charcoal absorption, anion exchange chromatography (AEX), sizeexclusion chromatography (SEC), and hydrophilic interaction chromatography. [16] However, these purification methods require high initial equipment investment and hard to scale up.…”