Preparation of Labelled dopamine by 125I using Lactoperoxidase and N- Bromosuccinamide

Abstract

The present study was carried out to prepare 125 I-dopamine Tracer, using oxidizing agents such as Lactoperoxidase and NBromosuccinamide , which was purified by column sephadex G-75 and used to study the biodistribution in normal Albino Swiss mice and the results indicate the possibility of using [125 I] iodo dopamine as myocardial imaging agent. The conditions of radioiodination such as concentration of oxidizing agent, reaction time, concentration of Atenolol and effect of pH were studied to get maximum yield. Stability study of dopamine tracer was carried out.

Preparation and Biological Evaluation of Iodopromazine as a Radiopharmaceutical Model for Brain Imaging

“…Increasing promazine's amount above 200 µg/500 µl led to slightly decrease in the labeling yield reaching 91.7 ± 3.20 % at 500 µg/500 µl. These results can be explained by increasing the interaction between molecules of promazine and radioiodine till certain extent after which equilibrium was obtained [26,27]. Thus, 200 µg/500 µl of promazine was the optimum amount of substrate required to obtain maximum labeling yield.…”