Preparation of Lymphocyte-Specific Antibody From Anti-Lymphocyte Serum

Shigeno, N.; Hämmerling, Ulrich; Arpels, Claude; Boyse, E. A.; Old, L. J.

doi:10.1016/s0140-6736(68)90530-8

Cited by 64 publications

(18 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…T lymphocytes are produced under the influence of the thymus, whereas B lymphocytes arise from stem cells in the bone marrow and are formed in both normal and thymectomized subjects. Antisera directed toward lymphocyte surface antigens have been exploited in studies of the tissue distribution and function of T and B lymphocytes in the mouse (26)(27)(28)(29) and chicken (30). Relatively little is known about the antigenic differences between ontogenetically distinct lymphocyte populations in other species.…”

Section: Makeup Of Lymphocyte Populations In Txbmmentioning

confidence: 99%

Anatomical Distribution of T and B Lymphocytes in the Rat

Goldschneider

McGregor

1973

The Journal of Experimental Medicine

104

View full text Add to dashboard Cite

Two major classes of lymphocytes have been shown to exist in the rat as they do in the mouse: thymus-derived (T) 1 lymphocytes and bone marrow-derived (B) lymphocytes. Rats thymectomized at birth show a selective deficiency of T lymphocytes in peripheral lymphoid tissue, blood, and lymph (1, 2), and an associated defect in cellmediated immunity as evidenced by failure of the residual lymphocytes to initiate delayed-type hypersensitivity reactions (3) and to respond vigorously to foreign histocompatibility antigens in vivo (3) or in vitro (4). Similar cellular and functional defects have been described in adult rats after either thymectomy and irradiation (5, 6) or chronic drainage of lymph and cells from a thoracic duct fistula (2, 7).Neonatal thymectomy and chronic lymph drainage have little effect upon B lymphocytes in the rat (1, 2), although antibody responses that depend upon the collaboration of T and B lymphocytes are often severely depressed (5-9). B lymphocytes have been distinguished from T lymphocytes by their buoyant density, decreased rate of incorporation of RNA precursors, and propensity to migrate to the "thymus-.ndependent" areas of peripheral lymphoid tissue (10,11). 1In the present study, antilymphocyte sera (ALS) of restricted specificity have been used to further delineate the tissue distribution of T and B lymphocytes in the rat3 The results indicate that antisera raised in rabbits against rat * This is publication number 53 from the

show abstract

Section: Makeup Of Lymphocyte Populations In Txbmmentioning

confidence: 99%

Anatomical Distribution of T and B Lymphocytes in the Rat

Goldschneider

McGregor

1973

The Journal of Experimental Medicine

104

View full text Add to dashboard Cite

show abstract

“…IgG from all sera was purified through a recombinant protein G-agarose column (BRL). After being washed three times with washing solution, the membranes were incubated with 1:2500 streptavidin-alkaline (10,11). In this way, the specific killing for WEHI-3 cells was 58% at a 1:4 dilution of the antiserum before absorption and 0% at a 1:4 dilution and 12% at a 1:2 dilution after absorption, whereas the specific killing for BL3 cells ranged from 95% to 100% at all dilutions examined from 1:0 to 1:128 before and after absorption.…”

Section: Methodsmentioning

confidence: 99%

Identification of a unique membrane-bound molecule on a hemopoietic stem cell line and on multipotent progenitor cells.

Han¹,

Chung²,

Wong³

1995

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Hemopoietic stem cells are a distinct population of cells that can differentiate into multilineages of hemopoietic cells and have long-term repopulation capability. A few membrane-bound molecules have been found to -be preferentially, but not uniquely, present on the surface of these primitive cells. We report here the identification of a unique 105-kDa glycoprotein on the surface of hemopoietic stem cell line BL3. This molecule, recognized by the absorbed antiserum, is not present on the surface of myeloid progenitors 32D and FDC-P1 cells, EL4 T cells, and NIH 3T3 fibroblasts. This antiserum can also be used to block the proliferation of BL3 cells even in the presence of mitogen-stimulated spleen cell conditioned medium, which is known to have a stimulating activity on BL3 cells. It can also inhibit development ofin vitro, fetal liver cell-derived multilineage colonies, but not other types of colonies, and of in vivo bone marrow cell-derived colony-forming unit spleen foci. These data suggest that gpiO5 plays an important role in hemopoietic stem cell differentiation.

show abstract

“…In contrast inter-species immunization involves a greater phylogenetic gap so it is to be expected that the recipient will recognize grosser antigenic disparities and ignore the finer ones (see discussion in Bennett et al 1972). This is probably why, for example, rabbit antiserum to mouse T-cells recognizes a 'species' antigen on these cells (MSLA) and does not distinguish the finer detail of Ly and other similar intra-species polymorphisms (Shigeno et al 1968). The greatest disparity is expected when the recipient completely lacks a molecule which the donor possesses.…”

Section: A Note On Weak Antigenicitymentioning

confidence: 99%

H‐Y Antigen: Genetics and Serology

Wachtel

1977

Immunological Reviews

View full text Add to dashboard Cite

Preparation of Lymphocyte-Specific Antibody From Anti-Lymphocyte Serum

Cited by 64 publications

References 2 publications

Anatomical Distribution of T and B Lymphocytes in the Rat

Anatomical Distribution of T and B Lymphocytes in the Rat

Identification of a unique membrane-bound molecule on a hemopoietic stem cell line and on multipotent progenitor cells.

H‐Y Antigen: Genetics and Serology

Contact Info

Product

Resources

About