Preparation of magnetic molecularly imprinted polymers for the identification of zearalenone in grains

J Animal Sci Biotechnol

et al. 2021

Mycotoxins are secondary metabolites produced by fungus. Many mycotoxin species are highly toxic and are frequently found in cereals and feedstuffs. So, powerful detection methods are vital and effective ways to prevent feed contamination. Traditional detection methods can no longer meet the needs of massive, real-time, simple, and fast mycotoxin monitoring. Rapid detection methods based on advanced material and sensor technology are the future trend. In this review, we highlight recent progress of mycotoxin rapid detection strategies in feedstuffs and foods, especially for simultaneous multiplex mycotoxin determination. Immunoassays, biosensors, and the prominent roles of nanomaterials are introduced. The principles of different types of recognition and signal transduction are explained, and the merits and pitfalls of these methods are compared. Furthermore, limitations and challenges of existing rapid sensing strategies and perspectives of future research are discussed.

Section: Molecularly Imprinted Polymers (Mips)mentioning

confidence: 99%

Section: Types Of Recognition Strategiesmentioning

confidence: 99%

Recent advances in immunoassays and biosensors for mycotoxins detection in feedstuffs and foods

Yang

J Animal Sci Biotechnol

et al. 2021

J of Separation Science

“…The Scatchard plots of HMMIPs and HMNIPs are shown in Figure 2D, in which two different fit curves with good linearity were observed at the Scatchard plots of HMMIPs. This suggests that the binding sites are heterogeneous, and there are two types of binding sites between HMMIPs and the target molecule: the specific and non-specific binding sites [13,24,25]. According to the slope and intercepts, the Q max values calculated by the formula were 44.30 mg/g and 6.07 mg/g for the higher affinity and lower affinity sites that can absorb the SMZ, respectively.…”

Section: Static Adsorption Performancementioning

confidence: 99%

Development of hydrophilic magnetic molecularly imprinted polymers for the dispersive solid‐phase extraction of sulfonamides from animal‐derived samples before HPLC detection

Zhao

Lü

Zhu

et al. 2021

Highly hydrophilic magnetic molecularly imprinted polymers were prepared through a surface imprinting technique for dispersive solid-phase extraction coupled with high-performance liquid chromatography to detect trace levels of ten sulfonamides in animal-derived samples. The obtained imprinted polymers were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, and adsorption experiments, which exhibited excellent specific adsorption for template sulfamethazine in aqueous solution. Moreover, the adsorption process could be completed within 25 min. Under the optimum conditions, the method exhibits good linear performance in the range of 5-to 10 mg/L, limits of detection ranging from 0.57 to 1.50 μg/L, and good recoveries of 85.09-110.93% in the spiked samples (chicken, cow milk, and goat milk). The proposed detection method not only avoids the use of organic solvents but also simplifies the pretreatment procedure via excellent magnetic properties. Furthermore, the method shows great potential for the rapid detection of drug residues.

“…The recovery and LOD of the developed method were compared to other reported methods [6,10,[25][26][27][28] for detecting ZEN. The results listed in Supporting information Table S2 indicated that the developed method has a low detection limit and a high recovery rate, which are similar to those reported for MSPE and SPE methods.…”

Section: Analysis Of Zearalenone In Real-world Samplesmentioning

confidence: 99%

Single‐crystal ordered macroporous metal‐organic framework as support for molecularly imprinted polymers and their integration in membrane formant for the specific recognition of zearalenone

Qiu

J of Separation Science

et al. 2021

Zearalenone is a fungal contaminant that is widely present in grains. Here, a novel molecularly imprinted membrane based on SOM-ZIF-8 was developed for the rapid and highly selective identification of zearalenone in grain samples. The molecularly imprinted membrane was prepared using polyvinylidene fluoride, cyclododecyl 2,4-dihydroxybenzoate as a template and SOM-ZIF-8 as a carrier. The factors influencing the extraction of zearalenone using this membrane, including the solution pH, extraction time, elution solvent, elution time, and elution volume, were studied in detail. The optimized conditions were 5 mL of sample solution at pH 6, extraction time of 45 min, 4 mL of acetonitrile:methanol = 9:1 as elution solvent, and elution time of 20 min. This method displayed a good linear range of 12-120 ng/g (R 2 = 0.998) with the limits of detection and quantification of this method are 1.7 and 5.5 ng/g, respectively. In addition, the membrane was used to selectively identify zearalenone in grain samples with percent recoveries ranging from 87.9 to 101.0% and relative standard deviation of less than 6.6%. Overall, this study presents a simple and effective chromatographic pretreatment method for detecting zearalenone in food samples.