Preparation of magnetically responsive albumin nanospheres and<i>in vitro</i>drug release studies

Ak, Güliz; Yılmaz, Habibe; Şanlıer, Şenay Hamarat

doi:10.3109/21691401.2013.768627

Cited by 13 publications

(11 citation statements)

References 35 publications

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“…Cellular uptake of nanoparticles and large molecules is generally accepted to be by an endocytosis mechanism through which they accumulate in the acidic compartments of early endosomes and then trafficked to the lysosomes . As we showed in the earlier study , DOX release from nanoparticles in acidic medium is faster. Therefore, we can suggest that after nanoparticles internalized into cells, DOX released from nanoparticles in the acidic intracellular organelles.…”

Section: Resultssupporting

confidence: 64%

“…Magnetization value of M-DOX-BSA-NPs was found experimentally 2.5 emu/g at room temperature when 500 G of magnetic field was applied. XRD pattern analysis also has confirmed Fe 3 O 4 structure in albumin nanoparticles (5). These data have demonstrated that M-DOX-BSA-NPs are responsive to the magnetic field and have ideal properties as a drug carrier.…”

Section: Resultssupporting

confidence: 54%

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In vitro Evaluation of Doxorubicin‐Incorporated Magnetic Albumin Nanospheres

Zeybek

Şanlı‐Mohamed

et al. 2014

Chem Biol Drug Des

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Magnetic albumin nanospheres that incorporate doxorubicin (M-DOX-BSA-NPs) were prepared previously by our research group to develop magnetically responsive drug carrier system. This nanocarrier was synthesized as a drug delivery system for targeted chemotherapy. In this work, cytotoxic effects of doxorubicin (DOX)-loaded/unloaded or magnetic/non-magnetic nanoparticles and free DOX against PC-3 cells and A549 cells were determined with the MTT test and the results were compared with each other. DOX-loaded magnetic albumin nanospheres (M-DOX-BSA-NPs) were found more cytotoxic than other formulations. The quantitative data obtained from flow cytometry analysis further verified the higher targeting and killing ability of M-DOX-BSA-NPs than free DOX on both of the cancer cell lines. Additionally, the results of cell cycle analysis have showed that M-DOX-BSA-NPs affected G1 and G2 phases. Finally, cell images were obtained using spin-disk confocal microscopy, and cellular uptake of M-DOX-BSA-NPs was visualized. The findings of this study suggest that M-DOX-BSA-NPs represent a potential doxorubicin delivery system for targeted drug transport into prostate and lung cancer cells.

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Section: Resultssupporting

confidence: 64%

Section: Resultssupporting

confidence: 54%

In vitro Evaluation of Doxorubicin‐Incorporated Magnetic Albumin Nanospheres

Zeybek

Şanlı‐Mohamed

et al. 2014

Chem Biol Drug Des

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“…Albumin at F has an increased viscosity and decreased dissolvability, 26 which aids its formation into nanospheres. Transmission electron microscopy ( Figure 1) showed FA-DOX-BSA MNPs to be approximately round in shape, and this has also been demonstrated by Ak et al 27 and Shen et al 28 The particles were uniform in size. Moreover, an Fe 3 O 4 area in the particle center could be seen in all particles.…”

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confidence: 56%

Preparation of folic acid-conjugated, doxorubicin-loaded, magnetic bovine serum albumin nanospheres and their antitumor effects in vitro and in vivo

Yang¹,

Miao³

et al. 2014

IJN

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Background This study aimed to generate targeted folic acid-conjugated, doxorubicin-loaded, magnetic iron oxide bovine serum albumin nanospheres (FA-DOX-BSA MNPs) that lower the side effects and improve the therapeutic effect of antitumor drugs when combined with hyperthermia and targeting therapy. A new nanodrug using magnetic nanospheres for heating and addition of the folate receptor with cancer cell specificity was prepared. The characteristics of these nanospheres and their antitumor effects in nasopharyngeal carcinoma were explored. Methods FA-DOX-BSA MNPs comprising encapsulated magnetic iron oxide nanoparticles were prepared using a desolvation cross-linking method. Activated folic acid (N-hydroxysuccinimide ester of folic acid) was conjugated to the surface of albumin nanospheres via amino groups. Results Folic acid was successfully expressed on the surface of the nanospheres. Electron microscopy revealed that the FA-DOX-BSA MNPs were nearly spherical and uniform in size, with an average diameter of 180 nm. The nanomaterial could deliver doxorubicin at clinically relevant doses with an entrapment efficiency of 80%. An increasing temperature test revealed that incorporation of magnetic iron oxide into nanospheres could achieve a satisfactory heat treatment temperature at a significantly lower dose when placed in a high-frequency alternating magnetic field. FA-DOX-BSA MNPs showed greater inhibition of tumors than in the absence of folic acid in vitro and in vivo. Compared with chemotherapy alone, hyperthermia combined with chemotherapy was more effective against tumor cells. Conclusion Folic acid-conjugated bovine serum albumin nanospheres composed of mixed doxorubicin and magnetic iron oxide cores can enable controlled and targeted delivery of anticancer drugs and may offer a promising alternative to targeted doxorubicin therapy for nasopharyngeal carcinoma.

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“…Albumin is another commonly used and clinically relevant stabilizing agent seen in various drug nanoparticle or drug formulations. In a single step, magnetic nanoparticles decorated by albumin, which is cross-linked using glutaraldehyde, were suitable for providing a uniform nanoparticle system for prostate cancer [24,30]. Similarly, liposome [15,31,32], hybrid [33], and dual/multilayer can serve as layers for stable nanoformulations.…”

Section: Magnetic Nanoparticlesmentioning

confidence: 99%

Magnetic nanoformulations for prostate cancer

Chowdhury

Roberts

Khan

et al. 2017

Drug Discovery Today

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Magnetic nanoparticles (MNPs) play a vital role for improved imaging applications. Recently, a number of studies demonstrate MNPs can be applied for targeted delivery, sustained release of therapeutics, and hyperthermia. Based on stable particle size and shape, biocompatibility, and inherent contrast enhancement characteristics, MNPs have been encouraged for pre-clinical studies and human use. As a theranostic platform development, MNPs need to balance both delivery and imaging aspects. Thus, this review provides significant insight and advances in the theranostic role of MNPs through the documentation of unique magnetic nanoparticles used in prostate cancer, their interaction with prostate cancer cells, in vivo fate, targeting, and biodistribution. Specific and custom-made applications of various novel nanoformulations in prostate cancer are discussed.

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Preparation of magnetically responsive albumin nanospheres andin vitrodrug release studies

Cited by 13 publications

References 35 publications

In vitro Evaluation of Doxorubicin‐Incorporated Magnetic Albumin Nanospheres

In vitro Evaluation of Doxorubicin‐Incorporated Magnetic Albumin Nanospheres

Preparation of folic acid-conjugated, doxorubicin-loaded, magnetic bovine serum albumin nanospheres and their antitumor effects in vitro and in vivo

Magnetic nanoformulations for prostate cancer

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