Preparation of mechanically patterned hydrogels for controlling the self-condensation of cells

Matsuzaki, Takahisa; Kawano, Yuma; Horikiri, Momoka; Shimokawa, Yuko; Yamazaki, Takashi; Okuma, Nao; Koike, Hiroyuki; Kimura, Masaki; Kawamura, Ryuzo; Yoneyama, Yosuke; Furuichi, Yasuro; Hakuno, Fumihiko; Takahashi, Shin-Ichiro; Nakabayashi, Seiichiro; Okamoto, Satoshi; Nakauchi, Hiromitsu; Taniguchi, Hideki; Takebe, Takanori; Yoshikawa, Hiroshi Y.

doi:10.1016/j.xpro.2023.102471

Cited by 1 publication

(1 citation statement)

References 17 publications

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“…Kinesin and tubulin protein materials in microtubules (MTs) monomeric form were purified from E. coli transformed with recombinant DNA and from porcine brain, respectively. 18,19) Seaplaque TM GTG TM Agarose was purchased from Lonza (ME). Balsam/ xylene solution was purchased from Yoshida Biological Laboratory (Japan).…”

Section: Introductionmentioning

confidence: 99%

Label-free 3D tracking of biological micro-objects using RGB pinholes

Kim,

Matsuzaki,

Yoshikawa

et al. 2024

Jpn. J. Appl. Phys.

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Defocusing digital particle image velocimetry (DDPIV) is a method developed within the field of fluidics to measure the velocities of particles within a volume through image analysis. For three-dimensional (3D) tracking, approaches utilizing defocusing techniques have been proposed to enable depthwise positional measurements. Recently, there have been suggestions to simplify this approach by employing a triple pinhole mask and to further enhance it with color filters that allow distinguish particles, even under dense conditions. This paper presents an attempt to apply the defocusing method using a triple pinhole mask with red, green, and blue (RGB) color-coding filters, demonstrating the successful label-free 3D tracking of biological micro-objects at multiple positions within a volume. The application of this method in fluidics and its potential for biological analysis are highlighted through successful examples of 3D tracking involving micro-particles influenced by motor proteins, dividing cells, and swimming microorganisms.

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Section: Introductionmentioning

confidence: 99%