Preparation of microspheres using poly-3-hydroxybutyrate biopolymer and its characterization

Abstract

Aim :Methodology : Results :Interpretation :Biodegradable poly-3-hydroxybutyrate is one of the most common biopolymer which is used in different fields such as medicine, agriculture, textile, industrial and food packaging. PHB microspheres are useful for targeting drugs to specific infection sites and for prolonged drug release. The present study focus on microsphere preparation for effective controlled drug release using poly-3-hydroxy butyrate biopolymer.In the present study, poly-3-hydroxy butyrate microspheres were prepared using solvent evaporation technique and characterized by SEM and FTIR. Poly-3-hydroxy butyrate microspheres were encapsulated with BSA and gentamicin. Drug encapsulation efficiency was determined. drug release profile and cytotoxicity was also studied.The prepared microspheres were of 2μm in size. Microspheres fabricated with 1% polyvinylalcohol showed encapsulation efficiency of 94.3% and 90.27% with BSA and gentamicin, respectively. The release studies in simulated body fluid, phosphate buffered saline and contact lens solution showed initial burst release followed by controlled release. cytotoxicity analysis showed 98% and 95% viability of cells in 3T3L1 cell line for microsphere encapsulated with BSA and gentamicin, respectively.Poly-3-hydroxy butyrate microspheres were found to release BSA and gentamicin in a controlled manner and were found to be non-toxic by cytotoxicity studies. In vitro in vitro in vitro In vitro in vitroJournal Home page : www.jeb.co.in « E-mail : editor@jeb.co.in Journal of Environmental BiologyPreparation of microspheres using poly-3-hydroxybutyrate biopolymer and its characterization Surface morphology of PHB microspheres was analyzed using scanning electron microscope (HITACHI model S-3000H).PHB polymer and PHB microspheres were subjected to Fourier Transform Infrared Spectroscopy analysis by FTIR spectrophotometer. Spectra were documented in 4000 cm to 400 cm range Two mg of microspheres were treated with 2 ml of 0.5 M sodium hydroxide for 15 min at 60ºC. To the mixture, 900 of 1 N hydrochloric acid was added to neutralize the samples and the volume was adjusted to 5 ml with distilled water. To the mixture, 3 ml of 0.65M boric acid, 0.5 ml iodine solution and 1.5 ml of distilled water were added. After 15 min incubation, absorbance was measured at 690 nm.Microsphere preparation (1% PVA at 800 rpm) procedure was followed with addition of BSA and gentamicin along with PHB (Shishatskaya ., 2008) Five mg of BSA and gentamicin loaded microspheres were immersed separately in 3 ml of contact lens solution, phosphate buffer (PBS) (pH, 7.2) and simulated body fluid (SBF) (Kokubo ., 1990). The Composition of simulated body fluid consisted pure water, sodium chloride, sodium bicarbonate, potassium chloride, potassium phosphate dibasic trihydrate, magnesium chloride hexahydrate, calcium chloride, Sodium sulphate and Tris base. They were kept in a shaker at 250 rpm at 37ºC. About 1ml of the sample was collected at regular intervals, till 24hrs. Each sample was ...

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